Chemical Compounds 251

ABSTRACT

The invention relates to chemical compounds of formula I, 
     
       
         
         
             
             
         
       
     
     and salts thereof. In some embodiments, the invention relates to inhibitors or modulators of PIM-1 and/or PIM-2, and/or PIM-3 protein kinase activity or enzyme function. In still further embodiments, the invention relates to pharmaceutical compositions comprising compounds disclosed herein, and their use in the prevention and treatment of PIM kinase related conditions and diseases, preferably cancer.

This application is a continuation of U.S. application Ser. No. 13/000,138, issuing, filed on May 25, 2011, which claims the benefit under 35 U.S.C. §119(a)-(d) of International Application No. PCT/GB2009/050773, filed on Jul. 2, 2009, which claims the benefit of U.S. Application No. 61/183,278, filed Jun. 2, 2009 and U.S. Patent Application No. 61/077,639, filed Jul. 2, 2008. The contents of each of the foregoing applications are incorporated herein by reference in their entirety.

FIELD OF INVENTION

The invention relates to chemical compounds of formula I,

and salts thereof. In some embodiments, the invention relates to inhibitors or modulators of PIM-1 and/or PIM-2, and/or PIM-3 protein kinase activity or enzyme function. In still further embodiments, the invention relates to pharmaceutical compositions comprising compounds disclosed herein, and their use in the prevention and treatment of PIM kinase related conditions and diseases, preferably cancer.

BACKGROUND

PIM-1 gene was first identified as a proviral insertion site in Moloney murine leukemia virus-induced T-cell lymphoma. PIM-1 gene translates a Ser/Thr protein kinase. The known PIM kinase family also includes PIM-2 and PIM-3. Mice studies suggest that physiologically the PIM kinases are involved in growth factor and cytokine signaling. Deregulated PIM kinase expression occurs a in large number of hematopoietic tumors, such as myeloid and lymphoblastic leukemias and lyphomas. PIM kinases are also expressed in solid tumors, such as prostate cancer and pancreatic cancer, and transgenic mice which express PIM-1 develop T-cell lymphoma. Dhanasekaran et al., (2001). Nature 412: 822-826 and Li et al., (2006) Cancer Res 66: 6741-6747. Accordingly, it is believed that PIM Kinase inhibitors will be useful in the treatment and/or prevention of cancer. Thus, there is a need to identify inhibitors of PIM kinases.

SUMMARY OF INVENTION

The invention relates to chemical compounds of formula I,

and salts thereof. In some embodiments, the invention relates to inhibitors or modulators of PIM-1 and/or PIM-2, and/or PIM-3 protein kinase activity or enzyme function. In still further embodiments, the invention relates to pharmaceutical compositions comprising compounds disclosed herein, and their use in the prevention and treatment of PIM kinase related conditions and diseases, preferably cancer.

DETAILED DESCRIPTION

The invention relates to a method of treating or preventing cancer comprising,

a) providing a pharmaceutical composition comprising a compound of formula I,

or pharmaceutically acceptable salts thereof, functioning to inhibit a PIM kinase, wherein

R¹ is selected from a carbocyclyl, aryl, and heterocyclyl, wherein R¹ is optionally substituted with one or more, the same or different, R²;

R² is selected from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfamoyl, arylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R² is optionally substituted with one or more, the same or different, R³;

R³ is selected from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, carbocyclyl, aryl, and heterocyclyl,

wherein R³ is optionally substituted with one or more, the same or different, R⁴;

R⁴ is selected from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, carbocyclyl, aryl, and heterocyclyl, wherein R⁴ is optionally substituted with one or more, the same or different, R⁵; and

R⁵ is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl; and

b) administering said pharmaceutical composition to a subject diagnosed with, exhibiting symptoms of, or at risk for cancer with the proviso that said compound of formula I is not 5-[[2-[(2,6-dichloro-3-methylphenyl)amino]phenyl]methylene]-2,4-thiazolidinedione.

In some embodiments, the invention relates to a method of treating or preventing cancer comprising,

a) providing a pharmaceutical composition comprising a compound of formula I,

or salts thereof, functioning to inhibit a PIM kinase, wherein

R¹ is selected from a carbocyclyl, aryl, and heterocyclyl, wherein R¹ is optionally substituted with one or more, the same or different, R²;

R² is selected from halogen, C₁₋₆alkyl, halogenated C₁₋₆alkyl, amino, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, and heterocyclyl, wherein R² is optionally substituted with one or more, the same or different, R³;

R³ is selected from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, carbocyclyl, aryl, and heterocyclyl, wherein R³ is optionally substituted with one or more, the same or different, R⁴;

R⁴ is selected from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, carbocyclyl, aryl, and heterocyclyl, wherein R⁴ is optionally substituted with one or more, the same or different, R⁵; and

R⁵ is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl; and

b) administering said pharmaceutical composition to a subject diagnosed with, exhibiting symptoms of, or at risk for cancer under conditions such that cancer is reduced or prevented.

In further embodiments, the invention relates to a method of treating cancer comprising administering to a subject in need thereof a pharmaceutical composition comprising a compound of the formula disclosed herein, wherein said cancer is selected from a leukemia, lymphoma, prostate cancer, pancreatic cancer or other solid tumors.

In further embodiments, the invention relates to a compound of formula IA,

or salts thereof, wherein,

--- is individually at each occurrence selected from a single and double bond;

n is selected from 0, 1, or 2;

m is selected from 0, 1, or 2;

A is selected from N and CR⁷;

X is selected from O, S, CHR¹⁰ and NR¹¹;

Y is selected from N, CH, and C;

R⁵, R⁶, and R⁷ are each individually and independently from hydrogen, C₁₋₆alkyl, halogen, cyano, nitro, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, carbocyclyl, aryl, and heterocyclyl, wherein R⁵, R⁶, and R⁷ are each optionally substituted with one or more, the same or different, R¹²;

R⁸ and R⁹ are each individually and independently selected from hydrogen, amino, hydroxyl, mercapto, C₁₋₆alkyl, C₁₋₆alkylamino, carbocyclyl, aryl, and heterocyclyl wherein R⁸ and R⁹ are each optionally substituted with one or more, the same or different, R¹⁵;

R¹⁰ is selected from hydrogen, C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfamoyl, arylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹⁰ is optionally substituted with one or more, the same or different, R¹²;

R¹¹ is selected from hydrogen, formyl, C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, C₁₋₆alkoxycarbonyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹¹ is optionally substituted with one or more, the same or different, R¹²;

R¹² is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹² is optionally substituted with one or more, the same or different, R¹⁶;

R¹⁵ is selected from C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, and C₁₋₆alkoxycarbonyl wherein R¹⁵ is optionally substituted with one or more, the same or different, R¹²;

R¹⁶ is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, aminomethyl, aminoethyl, aminopropyl, aminobutyl, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, and N-methyl-N-ethylsulfamoyl; and

provided that R⁵, R⁶, and R⁷ are not all hydrogen and

provided the compound is not 5-((5-nitro-2-(1-piperidinyl)phenyl)methylene)-2,4-thiazolidinedione or 5-((2-(4-morpholinyl)-5-nitrophenyl)methylene)-2,4-thiazolidinedione.

In some embodiments, the invention relates to a compound of formula ID,

or salts thereof, wherein,

n is selected from 0, 1, or 2;

A is selected from N and CR⁷;

X is selected from O, S, CHR¹⁰ and NR¹¹;

R⁵, R⁶, and R⁷ are each individually and independently from hydrogen, C₁₋₆alkyl, halogen, cyano, nitro, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, carbocyclyl, aryl, and heterocyclyl, wherein R⁵, R⁶, and R⁷ are each optionally substituted with one or more, the same or different, R¹²;

R⁸ and R⁹ are each individually and independently selected from hydrogen, amino, hydroxyl, mercapto, C₁₋₆alkyl, C₁₋₆alkylamino, carbocyclyl, aryl, and heterocyclyl wherein R⁸ and R⁹ are each optionally substituted with one or more, the same or different, R¹⁵;

R¹⁰ is selected from hydrogen, C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfamoyl, arylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹⁰ is optionally substituted with one or more, the same or different, R¹²;

R¹¹ is selected from hydrogen, formyl, C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, C₁₋₆alkoxycarbonyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹¹ is optionally substituted with one or more, the same or different, R¹²;

R¹² is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹² is optionally substituted with one or more, the same or different, R¹⁶;

R¹⁵ is selected from C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, and C₁₋₆alkoxycarbonyl wherein R¹⁵ is optionally substituted with one or more, the same or different, R¹²;

R¹⁶ is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, aminomethyl, aminoethyl, aminopropyl, aminobutyl, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, and N-methyl-N-ethylsulfamoyl.

In some embodiments with regard to any of the compound formula provided herein, R⁵, R⁶, and R⁷ are not all hydrogen.

In some embodiments with regard to any of the compound formula provided herein, R⁵, R⁶, and R⁷ are not 5-((5-nitro-2-(1-piperidinyl)phenyl)methylene)-2,4-thiazolidinedione or 5-((2-(4-morpholinyl)-5-nitrophenyl)methylene)-2,4-thiazolidinedione.

In some embodiments, the invention relates to a compound of formula IB,

or salts thereof, wherein,

n is selected from 0, 1, or 2;

X is selected from O, CHR¹⁰ and NR¹¹;

R⁵, R⁶, and R⁷ are each individually and independently from hydrogen, halogen, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, and N-methyl-N-ethylsulfamoyl;

R⁸ and R⁹ are each individually and independently selected from hydrogen, amino, C₁₋₆alkyl, C₁₋₆alkylamino, wherein R⁸ and R⁹ are each optionally substituted with one or more, the same or different, R¹⁵;

R¹⁰ is selected from hydrogen, C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfamoyl, arylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹⁰ is optionally substituted with one or more, the same or different, R¹²;

R¹¹ is selected from hydrogen, C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, C₁₋₆alkoxycarbonyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹¹ is optionally substituted with one or more, the same or different, R¹²;

R¹² is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹² is optionally substituted with one or more, the same or different, R¹⁶;

R¹⁵ is selected from C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, and C₁₋₆alkoxycarbonyl wherein R¹⁵ is optionally substituted with one or more, the same or different, R¹²;

R¹⁶ is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, and N-methyl-N-ethylsulfamoyl.

In some embodiments, with regard to any of the compound formula provided herein, said compound is not, 5-[[2-(4-methyl-1-piperazinyl)phenyl]methylene]-2,4-thiazolidinedione or 5-[[2-[(2,6-dichloro-3-methylphenyl)amino]phenyl]methylene]-2,4-thiazolidinedione.

In further embodiments, the invention relates to a compound of formula IC,

or salts thereof, wherein,

n is selected from 0, 1, 2, 3, 4, 5, 6, 7 or 8;

X is selected from O, S, CHR¹⁰ and NR¹¹;

Y is selected from O, S, and NR¹³;

R⁵, R⁶, and R⁷ are each individually and independently from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, C₁₋₆dialkylamino, carbocyclyl, aryl, and heterocyclyl, wherein R⁵, R⁶, and R⁷ are each optionally substituted with one or more, the same or different, R¹²;

R¹⁰ is selected from hydrogen, C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfamoyl, arylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹⁰ is optionally substituted with one or more, the same or different, R¹²;

R¹¹, R¹³, and R¹⁴ are each individually and independently selected from hydrogen, C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, and C₁₋₆alkoxycarbonyl wherein R¹¹ is optionally substituted with one or more, the same or different, R¹²;

or R¹¹ and R¹⁴, taken together with the atoms to which they are attached form a five, six, or seven membered heterocyclic ring optionally substituted with one or more, the same or different, R¹²;

R¹² is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl.

In some embodiments with regard to any of the compound formula provided herein, Y is NR¹³, wherein R¹³ is a C₁₋₆alkyl.

In some embodiments with regard to any of the compound formula provided herein, R⁵ is a halogenated C₁₋₆alkyl.

In some embodiments with regard to any of the compound formula provided herein, R⁷ is a halogen.

In some embodiments, the invention relates to a compound of formula IX,

or salts thereof, wherein

R⁵ is selected from hydrogen, C₁₋₆alkoxy, carbamoyl, and halogenated C₁₋₆alkyl;

R⁶ is selected from hydrogen, halogen, C₁₋₆alkoxy, and 2-(1-piperidyl)ethoxy;

R⁷ is selected from hydrogen, halogen, and C₁₋₆alkoxy;

R¹⁷ is a heterocarbocylcyl, wherein R¹⁷ is optionally substituted with one or more, the same or different, R¹⁸;

R¹⁸ is selected from halogen, formyl, amino, C₁₋₆alkyl, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, carbocyclyl, aryl, heterocyclyl, wherein R¹⁸ is optionally substituted with one or more, the same or different, R¹⁹;

R¹⁹ is selected from amino, C₁₋₆alkyl, hydroxy, carbocyclyl, and heterocyclyl wherein R¹⁹ is optionally substituted with one or more, the same or different, R²⁰; and

R²⁰ is selected from amino, C₁₋₆alkyl, and halogen.

In further embodiments, R¹⁷ is selected from (3R)-3-aminopyrrolidin-1-yl, (3R)-3-dimethylaminopyrrolidin-1-yl, (3S)-3-(3-aminopropylamino)pyrrolidin-1-yl, (3S)-3-(5-aminopentanoylamino)pyrrolidin-1-yl, (3S)-3-amino-1-piperidyl, (3S)-3-aminopyrrolidin-1-yl, (3S)-3-dimethylaminopyrrolidin-1-yl, (3S,5R)-3,5-dimethylpiperazin-1-yl, 1,4-diazepan-1-yl, 2-(1-piperidyl)ethoxy, 2-diethylaminoethoxy, 2-dimethylaminoethyl-methyl-amino, 2-hydroxyethoxy, 2-morpholinoethoxy, 3-(2-aminoethylamino)pyrrolidin-1-yl, 3-(2-hydroxyethylamino)pyrrolidin-1-yl, 3-(2-methylaminoethylamino)pyrrolidin-1-yl, 3-(3-aminopropanoylamino)pyrrolidin-1-yl, 3-(3-aminopropylamino)pyrrolidin-1-yl, 3-(3-methyl-1,2,4-oxadiazol-5-yl)-1-piperidyl, 3-(aminomethyl)-1-piperidyl, 3-(aminomethyl)pyrrolidin-1-yl, 3-acetamidopyrrolidin-1-yl, 3-aminopyrrolidin-1-yl, 3-dimethylaminopropoxy, 3-dimethylaminopropyl-methyl-amino, 3-dimethylaminopyrrolidin-1-yl, 3-hydroxypyrrolidin-1-yl, 3-pyridyl, 4-(1-methyl-4-piperidyl)piperazin-1-yl, 4-(1-piperidyl)-1-piperidyl, 4-(2-aminoethyl)piperazin-1-yl, 4-(2-hydroxyethyl)-1,4-diazepan-1-yl, 4-(2-hydroxyethyl)-1-piperidyl, 4-(2-hydroxyethyl)piperazin-1-yl, 4-(2-methylaminoethyl)piperazin-1-yl, 4-(2-morpholinoethyl)piperazin-1-yl, 4-(3-aminopropanoyl)-1,4-diazepan-1-yl, 4-(3-aminopropanoyl)piperazin-1-yl, 4-(3-aminopropyl)piperazin-1-yl, 4-(3-hydroxypropyl)piperazin-1-yl, 4-(3-methyl-1,2,4-oxadiazol-5-yl)-1-piperidyl, 4-(4-aminobutanoyl)-1,4-diazepan-1-yl, 4-(4-aminobutanoyl)piperazin-1-yl, 4-(4-chloro-2-fluoro-phenyl)piperazin-1-yl, 4-(4-fluorophenyl)piperazin-1-yl, 4-(4-pyridylmethyl)piperazin-1-yl, 445-aminopentanoyl)-1,4-diazepan-1-yl, 4-(5-aminopentanoyl)piperazin-1-yl, 4-(azetidine-3-carbonyl)piperazin-1-yl, 4-(benzo[1,3]dioxol-5-ylmethyl)piperazin-1-yl, 4-(cyclopropylmethyl)piperazin-1-yl, 4-(hydroxymethyl)-1-piperidyl, 4-(piperidine-3-carbonyl)-1,4-diazepan-1-yl, 4-(piperidine-3-carbonyl)piperazin-1-yl, 4-(piperidine-4-carbonyl)piperazin-1-yl, 4-[(2-chlorophenyl)methyl]piperazin-1-yl, 4-[3-(aminomethyl)benzoyl]piperazin-1-yl, 4-[4-(piperazin-1-ylmethyl)benzoyl]piperazin-1-yl, 4-acetylpiperazin-1-yl, 4-amino-1-piperidyl, 4-butyl-1,4-diazepan-1-yl, 4-cyclopentylpiperazin-1-yl, 4-dimethylamino-1-piperidyl, 4-hydroxy-1-piperidyl, 4-isobutylpiperazin-1-yl, 4-isopropylpiperazin-1-yl, 4-methylpiperazin-1-yl, 4-morpholino-1-piperidyl, 4-pyridyl, 4-pyrrolidin-1-yl-1-piperidyl, 4-tert-butoxycarbonylpiperazin-1-yl, 4-tert-butylpiperazin-1-yl, morpholino, piperazin-1-yl, and pyrrolidin-1-yl.

In further embodiments, the invention relates to a compound selected from:

-   5-({2-[(3S)-3-aminopiperidin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({2-[(3R)-3-aminopiperidin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-{[2-(4-aminopiperidin-1-yl)-3-chloro-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-({2-[3-(aminomethyl)piperidin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-{[3-chloro-2-(1,4-diazepan-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-{[3-chloro-2-(4-cyclopentylpiperazin-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(4-fluorophenyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(3-methyl-1,2,4-oxadiazol-5-yl)piperidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-{[3-chloro-2-(4-pyrrolidin-1-ylpiperidin-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(1-methylethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(2-methylpropyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(2-hydroxyethyl)piperidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(4-chloro-2-fluorophenyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[3-(3-methyl-1,2,4-oxadiazol-5-yl)piperidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(pyridin-4-ylmethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(2-chlorobenzyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(1-methylpiperidin-4-yl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(2-morpholin-4-ylethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(cyclopropylmethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-{[3-chloro-2-(4-morpholin-4-ylpiperidin-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(3-hydroxypropyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(dimethylamino)piperidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-{[3-chloro-2-{[3-(dimethylamino)propyl](methyl)amino}-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(2-hydroxyethyl)-1,4-diazepan-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-{[2-(4-butyl-1,4-diazepan-1-yl)-3-chloro-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-({3-chloro-2-[4-(2-hydroxyethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-{[3-chloro-2-morpholin-4-yl-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-{[2-(4-tert-butylpiperazin-1-yl)-3-chloro-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-{[2-(1,4′-bipiperidin-1′-yl)-3-chloro-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-{[3-chloro-2-(4-methylpiperazin-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   5-({3-bromo-2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione;     and -   5-({2-[(3R,5S)-3,5-dimethylpiperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione, -   (5Z)-5-({3-[3-(4-methylpiperazin-1-yl)propoxy]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   2-{3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenoxy}acetamide; -   (5Z)-5-{[3-(3-piperidin-1-ylpropoxy)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({3-[(4-methylpiperazin-1-yl)methyl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   N-[2-(dimethylamino)ethyl]-2′-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]-N-methylbiphenyl-4-sulfonamide; -   5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-methoxyphenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({5-chloro-2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-4-methylphenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-fluorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-methylphenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({5-bromo-2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-3-fluorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-({2-chloro-6-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-methoxyphenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-bromophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopyrrolidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-bromophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-ethoxyphenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(2-methylpropoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(cyclohexylmethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(cyclohexyloxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R,4R)-3-amino-4-hydroxypiperidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-{[3-chloro-2-(1,4-diazepan-1-yl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(1-methylethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-(1-methylethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-ethoxyphenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-{[4-(aminomethyl)benzyl]amino}piperidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({3-chloro-2-[(3R)-3-{[2-(methylamino)ethyl]amino}piperidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S,4S)-3-amino-4-hydroxypyrrolidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({3-chloro-2-[4-methyl-3-(methylamino)piperidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-{[2-(3-amino-4-methylpiperidin-1-yl)-3-chlorophenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(2,2,2-trifluoroethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-(2,2,2-trifluoroethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(2-methoxyethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-(2-methoxyethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(cyclopentyloxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(cyclobutyloxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   4-[(3R)-3-aminopiperidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]benzamide; -   4-[(3S)-3-aminopiperidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]benzamide; -   4-[(3R)-3-aminopiperidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]benzoic     acid; -   4-[(3S)-3-aminopyrrolidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]benzoic     acid; -   (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]biphenyl-3-yl}methylidene)-1,3-thiazolidine-2,4-dione; -   5-{[2-(3-aminopropoxy)-5-methoxyphenyl]methylidene}-1,3-thiazolidine-2,4-dione; -   N-{4-[3-(dimethylamino)pyrrolidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}acetamide; -   (5Z)-5-[(3-chloro-2-{(3R)-3-[(2-hydroxyethyl)amino]piperidin-1-yl}phenyl)methylidene]-1,3-thiazolidine-2,4-dione; -   (5Z)-5-[(3-chloro-2-{(3R)-3-[(3-hydroxypropyl)amino]piperidin-1-yl}phenyl)methylidene]-1,3-thiazolidine-2,4-dione; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-1-methyl-1H-imidazole-2-carboxamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-methoxyacetamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-1-methyl-1H-pyrazole-3-carboxamide; -   N²-carbamoyl-N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]glycinamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-pyridin-3-ylacetamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-pyridin-4-ylacetamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-1-methyl-1H-pyrazole-4-carboxamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-(1-oxidothiomorpholin-4-yl)acetamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-4-sulfamoylbutanamide; -   N′-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-N,N-dimethylbutanediamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-N˜2˜,N˜2˜-dimethylglycinamide; -   N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-cyanoacetamide; -   N²-acetyl-N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]glycinamide; -   (5Z)-5-({3-chloro-2-[(3R)-3-(dipropylamino)piperidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; -   (5Z)-5-[(3-chloro-2-{(3R)-3-[(3,3,3-trifluoropropyl)amino]piperidin-1-yl}phenyl)methylidene]-1,3-thiazolidine-2,4-dione; -   5-[(5-methoxy-2-{3-[(1-methylethyl)amino]propoxy}phenyl)methylidene]-1,3-thiazolidine-2,4-dione; -   (5Z)-5-({5-amino-2-[3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione;     and -   5-[(2-amino-4,5-dimethoxyphenyl)methylidene]-1,3-thiazolidine-2,4-dione,

or salts thereof.

In some embodiments, the invention relates to any of the compounds disclosed herein that are in the (Z) isomer.

In some embodiments, the invention relates to any of the compounds disclosed herein that are in the (E) isomer.

In some embodiments, the invention relates to compositions comprising a mixture of the (Z) and (E) isomers.

In some embodiments, the invention relates to a pharmaceutical composition comprising a substituted 5-(3-halo-2-[piperidin-1-yl]phenylmethylidene)-1,3-thiazolidine-2,4-dione functioning to inhibit a PIM kinase.

In further embodiments, the invention relates to a pharmaceutical composition comprising a compound of formula I, IA, IB, IC, ID, or IX or a pharmaceutically acceptable salt thereof.

In further embodiments, the invention relates to a method of inhibiting a PIM kinase comprising, providing a compound of formula I, IA, IB, IC, ID, or IX, as defined herein, and mixing a PIM kinase and said compound under conditions such that PIM kinase phosphorylation is inhibited.

In further embodiments, said method is an in vitro method.

In further embodiments, said method is an in vivo method.

In further embodiments, the invention relates to a method of inhibiting a PIM kinase in a subject comprising administering to the subject a therapeutically effective amount of a compound of any of the formula disclosed herein or a pharmaceutically acceptable salt thereof.

In further embodiments, said PIM kinase is selected from PIM-1, PIM-2, and PIM-3.

In further embodiments, the invention relates to the use of a compound of the formula I, IA, IB, IC, ID, or IX, or a pharmaceutically acceptable salt thereof, as defined herein, for the manufacture of a medicament for the production of a PIM kinase inhibitory effect in a subject.

In further embodiments, the invention relates to the use of a compound of the formula I, IA, IB, IC, ID, or IX, or a pharmaceutically acceptable salt thereof, as disclosed herein, for the manufacture of a medicament for the production of an anti-cancer effect in a subject.

In some embodiments, the invention relates to a method of making a compound of formula IA as defined herein,

or salt thereof, comprising

a) mixing a compound of formula XI,

-   -   or salt thereof, wherein E is a halogen, and R⁵, R⁶, and A are         defined herein, with a compound of formula XII,

or salt thereof, wherein

-   -   ---, R⁸, R⁹, n, m, Y and X are defined herein,     -   if Y is N, then R²¹ is hydrogen,     -   if Y is C, then R²¹ is selected from boronic acid and a boronic         ester, and     -   if Y is CH, then R²¹ is selected from a metal halide,

under conditions such that composition comprising a compound of formula XIII,

or salt thereof, is formed; and

b) mixing the compound of formula XIII and thiazolidine-2,4-dione under conditions such that a compound of formula IA is formed.

In further embodiments, said metal halide is selected from lithium chloride and magnesium bromide.

In further embodiments, said boronic ester is a dialkyl boronic ester, such as diethyl boronic ester, or a cyclic boronic ester, such as the boronic ester of 1,2-alkyldiols, such as 1,3,2-dioxaborolane, or cycloalkyldiols which may be optionally substituted.

In further embodiments, the invention relates to a method of making a compound of formula ID, or salt thereof comprising

a) mixing a compound of formula II,

or salt thereof, wherein R⁵, R⁶, and A are defined herein, with a compound of formula III,

or salt thereof, wherein R⁸, R⁹, n, and X are defined herein, under conditions such that composition comprising a compound of formula IV,

or salt thereof, is formed; and b) mixing the compound of formula IV and thiazolidine-2,4-dione under conditions such that a compound of formula ID is formed.

In further embodiments, the invention relates to a method of making a compound of formula IC, or salt thereof comprising a) mixing a compound of formula V,

or salt thereof, wherein R⁵, R⁶, and R⁷ are defined herein, with a compound of formula VI,

or salt thereof, wherein R¹⁴, n, X and Y are defined herein, under conditions such that a composition comprising a compound of formula VIII,

or salt thereof, is formed; and b) mixing the compound of formula VIII and thiazolidine-2,4-dione under conditions such that a compound of formula IC is formed.

In some embodiments, the invention relates to compounds of formula I, IA, IB, IC, ID, or IX provided that they are not selected from 5-((2-dipropylamino-5-nitrophenyl)methylene)-2,4-thiazolidinedione; 5-((5-nitro-2-(1-piperidinyl)phenyl)methylene)-2,4-thiazolidinedione; 5-((2-(4-morpholinyl)-5-nitrophenyl)methylene)-2,4-thiazolidinedione; 5-[[2-(4-methyl-1-piperazinyl)phenyl]methylene]-2,4-thiazolidinedione; 5-[[2-[(2,6-dichloro-3-methylphenyl)amino]phenyl]methylene]-2,4-thiazolidinedione; [2S-[2α(Z),4α]]-1-([1,1′-biphenyl]-4-ylsulfonyl)-N-[2-[(2,4-dioxo-5-thiazolidinylidene)methyl]phenyl]-4-(methylthio)-2-pyrrolidinemethanamine; 1,1-dimethylethyl ester [[2S-[2α(Z),4α]]-3-[[[1-([1,1′-biphenyl]-4-ylsulfonyl)-4-(methylthio)-2-pyrrolidinyl]methyl]amino]-4-[(2,4-dioxo-5-thiazolidinylidene)methyl]phenyl]carbamic acid; and [2S-[2α(Z),4α]]-N-[5-amino-2-[(2,4-dioxo-5-thiazolidinylidene)methyl]phenyl]-1-([1,1′-biphenyl]-4-ylsulfonyl)-4-(methylthio)-2-pyrrolidinemethanamine.

It is the Applicants understanding that WO 2001002377 discloses 5-((2-dipropylamino-5-nitrophenyl)methylene)-2,4-thiazolidinedione, 5-((5-nitro-2-(1-piperidinyl)phenyl)methylene)-2,4-thiazolidinedione, and 5-((2-(4-morpholinyl)-5-nitrophenyl)methylene)-2,4-thiazolidinedione.

It is the Applicants understanding that WO 9814433 discloses 5-[[2-(4-methyl-1-piperazinyl)phenyl]methylene]-2,4-thiazolidinedione.

It is the Applicants understanding that U.S. Pat. No. 6,211,209 discloses, 5-[[2-[(2,6-dichloro-3-methylphenyl)amino]phenyl]methylene]-2,4-thiazolidinedione.

It is the Applicants understanding that WO 9705135 discloses, [2S-[2α(Z),4α]]-1-([1,1′-biphenyl]-4-ylsulfonyl)-N-[2-[(2,4-dioxo-5-thiazolidinylidene)methyl]phenyl]-4-(methylthio)-2-pyrrolidinemethanamine, 1,1-dimethylethyl ester [[2S-[2α(Z),4α]]-3-[[[1-([1,1′-biphenyl]-4-ylsulfonyl)-4-(methylthio)-2-pyrrolidinyl]methyl]amino]-4-[(2,4-dioxo-5-thiazolidinylidene)methyl]phenyl]-carbamic acid, and [2S-[2α(Z),4α]]-N-[5-amino-2-[(2,4-dioxo-5-thiazolidinylidene)methyl]phenyl]-1-([1,1′-biphenyl]-4-ylsulfonyl)-4-(methylthio)-2-pyrrolidinemethanamine.

The preceding understandings are not intended to be admissions.

In some embodiments, compounds disclosed herein could be used in the clinic either as a single agent by itself or in combination with other clinically relevant agents. This compound could also prevent the potential cancer resistance mechanisms that may arise due to mutations in a set of genes.

The anti-cancer treatment defined herein may be applied as a sole therapy or may involve, in addition to the compound of the invention, conventional surgery or radiotherapy or chemotherapy. Such chemotherapy may include one or more of the following categories of anti-tumour agents:

(i) antiproliferative/antineoplastic drugs and combinations thereof, as used in medical oncology, such as alkylating agents (for example cis-platin, carboplatin, cyclophosphamide, nitrogen mustard, melphalan, chlorambucil, busulfan and nitrosoureas); antimetabolites (for example antifolates such as fluoropyrimidines like 5-fluorouracil and tegafur, raltitrexed, methotrexate, cytosine arabinoside and hydroxyurea); antitumour antibiotics (for example anthracyclines like adriamycin, bleomycin, doxorubicin, daunomycin, epirubicin, idarubicin, mitomycin-C, dactinomycin and mithramycin); antimitotic agents (for example vinca alkaloids like vincristine, vinblastine, vindesine and vinorelbine and taxoids like taxol and taxotere); and topoisomerase inhibitors (for example epipodophyllotoxins like etoposide and teniposide, amsacrine, topotecan and camptothecin); and proteosome inhibitors (for example bortezomib [Velcade®]); and the agent anegrilide [Agrylin®]; and the agent alpha-interferon

(ii) cytostatic agents such as antioestrogens (for example tamoxifen, toremifene, raloxifene, droloxifene and iodoxyfene), oestrogen receptor down regulators (for example fulvestrant), antiandrogens (for example bicalutamide, flutamide, nilutamide and cyproterone acetate), LHRH antagonists or LHRH agonists (for example goserelin, leuprorelin and buserelin), progestogens (for example megestrol acetate), aromatase inhibitors (for example as anastrozole, letrozole, vorazole and exemestane) and inhibitors of 5α-reductase such as finasteride;

(iii) agents which inhibit cancer cell invasion (for example metalloproteinase inhibitors like marimastat and inhibitors of urokinase plasminogen activator receptor function);

(iv) inhibitors of growth factor function, for example such inhibitors include growth factor antibodies, growth factor receptor antibodies (for example the anti-erbb2 antibody trastuzumab [Herceptin™] and the anti-erbb1 antibody cetuximab [C225]), farnesyl transferase inhibitors, tyrosine kinase inhibitors and serine/threonine kinase inhibitors, for example inhibitors of the epidermal growth factor family (for example EGFR family tyrosine kinase inhibitors such as:

-   N-(3-chloro-4-fluorophenyl)-7-methoxy-6-(3-morpholinopropoxy)quinazolin-4-amine     (gefitinib, AZD1839), -   N-(3-ethynylphenyl)-6,7-bis(2-methoxyethoxy)quinazolin-4-amine     (erlotinib, OSI-774), and -   6-acrylamido-N-(3-chloro-4-fluorophenyl)-7-(3-morpholinopropoxy)quinazolin-4-amine     (CI 1033),

for example inhibitors of the platelet-derived growth factor family and for example inhibitors of the hepatocyte growth factor family, for example inhibitors or phosphotidylinositol 3-kinase (PI3K) and for example inhibitors of mitogen activated protein kinase kinase (MEK1/2) and for example inhibitors of protein kinase B (PKB/Akt), for example inhibitors of Src tyrosine kinase family and/or Abelson (Abl) tyrosine kinase family such as AZD0530 and dasatinib (BMS-354825) and imatinib mesylate (Gleevec™); and any agents that modify STAT signaling.

(v) antiangiogenic agents such as those which inhibit the effects of vascular endothelial growth factor, (for example the anti-vascular endothelial cell growth factor antibody bevacizumab [Avastin™], compounds such as those disclosed in International Patent Applications WO 97/22596, WO 97/30035, WO 97/32856 and WO 98/13354) and compounds that work by other mechanisms (for example linomide, inhibitors of integrin αvβ3 function and angiostatin);

(vi) vascular damaging agents such as Combretastatin A4 and compounds disclosed in International Patent Applications WO 99/02166, WO 00/40529, WO 00/41669, WO 01/92224, WO 02/04434 and WO 02/08213;

(vii) antisense therapies, for example those which are directed to the targets listed above, such as ISIS 2503, an anti-ras antisense;

(viii) gene therapy approaches, including for example approaches to replace aberrant genes such as aberrant p53 or aberrant BRCA1 or BRCA2, GDEPT (gene-directed enzyme pro-drug therapy) approaches such as those using cytosine deaminase, thymidine kinase or a bacterial nitroreductase enzyme and approaches to increase patient tolerance to chemotherapy or radiotherapy such as multi-drug resistance gene therapy; and

(ix) immunotherapy approaches, including for example ex-vivo and in-vivo approaches to increase the immunogenicity of patient tumour cells, such as transfection with cytokines such as interleukin 2, interleukin 4 or granulocyte-macrophage colony stimulating factor, approaches to decrease T-cell anergy, approaches using transfected immune cells such as cytokine-transfected dendritic cells, approaches using cytokine-transfected tumour cell lines and approaches using anti-idiotypic antibodies, and approaches using the immunomodulatory drugs thalidomide and lenalidomide [Revlimid®].

Such conjoint treatment may be achieved by way of the simultaneous, sequential or separate dosing of the individual components of the treatment. Such combination products employ the compounds of this invention, or pharmaceutically acceptable salts thereof, within the dosage range described hereinbefore and the other pharmaceutically-active agent within its approved dosage range.

The invention relates to phosphorylation inhibitors of PIM kinases. In still further embodiments, the invention relates to pharmaceutical composition comprising compounds disclosed herein and their use in the prevention and treatment of cancer.

It is understood the compositions disclosed herein may exist in solid and solution form tautomers. For example, imidazole and imidazole containing heterocycles may be drawn in a formula such that one or the other of the nitrogens contain a hydrogen atom. However, as provided herein, embodiments of such a formula are considered to encompass alternative tautomeric forms.

As used herein, “alkyl” means a noncyclic straight chain or branched, unsaturated or saturated hydrocarbon such as those containing from 1 to 10 carbon atoms, while the term “lower alkyl” or “C₁₋₆alkyl” has the same meaning as alkyl but contains from 1 to 6 carbon atoms. The term “higher alkyl” has the same meaning as alkyl but contains from 7 to 10 carbon atoms. Representative saturated straight chain alkyls include methyl, ethyl, n-propyl, n-butyl, n-pentyl, n-hexyl, n-septyl, n-octyl, n-nonyl, and the like; while saturated branched alkyls include isopropyl, sec-butyl, isobutyl, tert-butyl, isopentyl, and the like. Unsaturated alkyls contain at least one double or triple bond between adjacent carbon atoms (referred to as an “alkenyl” or “alkynyl”, respectively). Representative straight chain and branched alkenyls include ethylenyl, propylenyl, 1-butenyl, 2-butenyl, isobutylenyl, 1-pentenyl, 2-pentenyl, 3-methyl-1-butenyl, 2-methyl-2-butenyl, 2,3-dimethyl-2-butenyl, and the like; while representative straight chain and branched alkynyls include acetylenyl, propynyl, 1-butyryl, 2-butyryl, 1-pentynyl, 2-pentynyl, 3-methyl-1-butynyl, and the like.

A “halogenated alkyl” refers to an alkyl group where one or more or all of the hydrogen(s) are substituted with halogen(s). A representative halogenated alkyl includes trifluoromethyl (i.e., —CF₃).

Non-aromatic mono or polycyclic alkyls are referred to herein as “carbocycles” or “carbocyclyl” groups. Representative saturated carbocycles include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and the like; while unsaturated carbocycles include cyclopentenyl and cyclohexenyl, aryls and the like.

“Heterocarbocycles” or heterocarbocyclyl” groups are carbocycles which contain from 1 to 4 heteroatoms independently selected from nitrogen, oxygen and sulfur which may be saturated or unsaturated (but not aromatic), monocyclic or polycyclic, and wherein the nitrogen and sulfur heteroatoms may be optionally oxidized, and the nitrogen heteroatom may be optionally quaternized. Heterocarbocycles include morpholinyl, pyrrolidinonyl, pyrrolidinyl, piperidinyl, hydantoinyl, valerolactamyl, oxiranyl, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, tetrahydropyridinyl, tetrahydroprimidinyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, tetrahydropyrimidinyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, and the like.

“Aryl” means an aromatic carbocyclic monocyclic or polycyclic ring such as phenyl or naphthyl.

As used herein, “heteroaryl” refers an aromatic heterocarbocycle having 1 to 4 heteroatoms selected from nitrogen, oxygen and sulfur, and containing at least 1 carbon atom, including both mono- and polycyclic ring systems. Polycyclic ring systems may, but are not required to, contain one or more non-aromatic rings, as long as one of the rings is aromatic. Representative heteroaryls are furyl, benzofuranyl, thiophenyl, benzothiophenyl, pyrrolyl, indolyl, isoindolyl, azaindolyl, pyridyl, quinolinyl, isoquinolinyl, oxazolyl, isooxazolyl, benzoxazolyl, pyrazolyl, imidazolyl, benzimidazolyl, thiazolyl, benzothiazolyl, isothiazolyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, cinnolinyl, phthalazinyl, and quinazolinyl. It is contemplated that the use of the term “heteroaryl” includes N-alkylated derivatives such as a 1-methylimidazol-5-yl substituent.

As used herein, “heterocycle” or “heterocyclyl” refers to mono- and polycyclic ring systems having 1 to 4 heteroatoms selected from nitrogen, oxygen and sulfur, and containing at least 1 carbon atom. The mono- and polycyclic ring systems may be aromatic, non-aromatic or mixtures of aromatic and non-aromatic rings. Heterocycle includes heterocarbocycles, heteroaryls, and the like.

“Alkylthio” refers to an alkyl group as defined above with the indicated number of carbon atoms attached through a sulfur bridge. An example of an alkylthio is methylthio, (i.e., —S—CH₃).

“Alkoxy” refers to an alkyl group as defined above with the indicated number of carbon atoms attached through an oxygen bridge. Examples of alkoxy include, but are not limited to, methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, s-butoxy, t-butoxy, n-pentoxy, and s-pentoxy. Preferred alkoxy groups are methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, s-butoxy, t-butoxy.

“Alkylamino” refers an alkyl group as defined above with the indicated number of carbon atoms attached through an amino bridge. An example of an alkylamino is methylamino, (i.e., —NH—CH₃).

“Alkanoyl” refers to an alkyl as defined above with the indicated number of carbon atoms attached through a carbonyl bride (i.e., —(C═O)alkyl).

“Alkylsulfonyl” refers to an alkyl as defined above with the indicated number of carbon atoms attached through a sulfonyl bridge (i.e., —S(═O)₂alkyl) such as mesyl and the like, and “Arylsulfonyl” refers to an aryl attached through a sulfonyl bridge (i.e., —S(═O)₂aryl).

“Alkylsulfamoyl” refers to an alkyl as defined above with the indicated number of carbon atoms attached through a sulfamoyl bridge (i.e., —NHS(═O)₂alkyl), and an “Arylsulfamoyl” refers to an alkyl attached through a sulfamoyl bridge (i.e., (i.e., —NHS(═O)₂aryl).

“Alkylsulfinyl” refers to an alkyl as defined above with the indicated number of carbon atoms attached through a sulfinyl bridge (i.e. —S(═O)alkyl).

The term “substituted” refers to a molecule wherein at least one hydrogen atom is replaced with a substituent. When substituted, one or more of the groups are “substituents.” The molecule may be multiply substituted. In the case of an oxo substituent (“═O”), two hydrogen atoms are replaced. Example substituents within this context may include halogen, hydroxy, alkyl, alkoxy, nitro, cyano, oxo, carbocyclyl, carbocycloalkyl, heterocarbocyclyl, heterocarbocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, —NR_(a)R_(b), —NR_(a)C(═O)R_(b), —NR_(a)C(═O)NR_(a)NR_(b), —NR_(a)C(═O)OR_(b), —NR_(a)SO₂R_(b), —C(═O)R_(a), —C(═O)OR_(a), —C(═O)NR_(a)R_(b), —OC(═O)NR_(a)R_(b), —OR_(a), —SR_(a), —SOR_(a), —S(═O)₂R_(a), —OS(═O)₂R_(a) and —S(═O)₂OR_(a). R_(a) and R_(b) in this context may be the same or different and independently hydrogen, halogen hydroxyl, alkyl, alkoxy, alkyl, amino, alkylamino, dialkylamino, carbocyclyl, carbocycloalkyl, heterocarbocyclyl, heterocarbocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl.

The term “optionally substituted,” as used herein, means that substitution is optional and therefore it is possible for the designated atom to be unsubstituted.

As used herein, the terms “prevent” and “preventing” include the prevention of the recurrence, spread or onset. It is not intended that the present invention be limited to complete prevention. In some embodiments, the onset is delayed, or the severity of the disease is reduced.

As used herein, the terms “treat” and “treating” are not limited to the case where the subject (e.g. patient) is cured and the disease is eradicated. Rather, embodiments, of the present invention also contemplate treatment that merely reduces symptoms, and/or delays disease progression.

As used herein, “salts” refer to derivatives of the disclosed compounds where the parent compound is modified making acid or base salts thereof. Examples of salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines, alkylamines, or dialkylamines; alkali or organic salts of acidic residues such as carboxylic acids; and the like. In preferred embodiment the salts are conventional non-toxic pharmaceutically acceptable salts including the quaternary ammonium salts of the parent compound formed, and non-toxic inorganic or organic acids. Preferred salts include those derived from inorganic acids such as hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, nitric and the like; and the salts prepared from organic acids such as acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, sulfanilic, 2-acetoxybenzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, isethionic, and the like.

The pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound that contains a basic or acidic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, p. 1418, the disclosure of which is hereby incorporated by reference.

“Subject” means any animal, preferably a human patient, livestock, or domestic pet.

“Cancer” refers any of various cellular diseases with malignant neoplasms characterized by the proliferation of cells. It is not intended that the diseased cells must actually invade surrounding tissue and metastasize to new body sites. Cancer can involve any tissue of the body and have many different forms in each body area. Within the context of certain embodiments, whether “cancer is reduced” may be identified by a variety of diagnostic manners known to one skill in the art including, but not limited to, observation the reduction in size or number of tumor masses or if an increase of apoptosis of cancer cells observed, e.g., if more than a 5% increase in apoptosis of cancer cells is observed for a sample compound compared to a control without the compound. It may also be identified by a change in relevant biomarker or gene expression profile, such as PSA for prostate cancer, her2 for breast cancer, or others.

The present pharmaceutical compositions can take the form of solutions, suspensions, emulsion, tablets, pills, pellets, capsules, capsules containing liquids, powders, sustained-release formulations, suppositories, emulsions, aerosols, sprays, suspensions, or any other form suitable for use.

Administration may be topical, i.e., substance is applied directly where its action is desired, enteral or oral, i.e., substance is given via the digestive tract, parenteral, i.e., substance is given by other routes than the digestive tract such as by injection.

In a preferred embodiment, the active compound and optionally another therapeutic or prophylactic agent are formulated in accordance with routine procedures as pharmaceutical compositions adapted for intravenous administration to human beings. Typically, the active compound for intravenous administration are solutions in sterile isotonic aqueous buffer. Where necessary, the compositions can also include a solubilizing agent. Compositions for intravenous administration can optionally include a local anesthetic such as lignocaine to ease pain at the site of the injection. Generally, the ingredients are supplied either separately or mixed together in unit dosage form, for example, as a dry lyophilized powder or water free concentrate in a hermetically sealed container such as an ampoule. Where the active compound is to be administered by infusion, it can be dispensed, for example, with an infusion bottle containing sterile pharmaceutical grade water or saline. Where the active compound is administered by injection, an ampoule of sterile water for injection or saline can be provided so that the ingredients can be mixed prior to administration.

Compositions for oral delivery can be in the form of tablets, lozenges, aqueous or oily suspensions, granules, powders, emulsions, capsules, syrups, or elixirs, for example. Orally administered compositions can contain one or more optional agents, for example, sweetening agents such as fructose, aspartame or saccharin; flavoring agents such as peppermint, oil of wintergreen, or cherry; coloring agents; and preserving agents, to provide a pharmaceutically palatable preparation. A time delay material such as glycerol monostearate or glycerol stearate can also be used. Oral compositions can include standard vehicles such as mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, magnesium carbonate, and the like. Such vehicles are preferably of pharmaceutical grade.

Compositions for use in accordance with the present invention can be formulated in conventional manner using one or more physiologically acceptable carriers or excipients. Thus, the compound and optionally another therapeutic or prophylactic agent and their physiologically acceptable salts and solvates can be formulated into pharmaceutical compositions for administration by inhalation or insufflation (either through the mouth or the nose) or oral, parenteral or mucosol (such as buccal, vaginal, rectal, sublingual) administration. In one embodiment, local or systemic parenteral administration is used.

For oral administration, the compositions can take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g., pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g., lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g., magnesium stearate, talc or silica); disintegrants (e.g., potato starch or sodium starch glycolate); or wetting agents (e.g., sodium lauryl sulfate). The tablets can be coated by methods well known in the art. Liquid preparations for oral administration can take the form of, for example, solutions, syrups or suspensions, or they can be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations can be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g., methyl or propyl-p-hydroxybenzoates or sorbic acid). The preparations can also contain buffer salts, flavoring, coloring and sweetening agents as appropriate.

EXPERIMENTAL

The following is intended to provide examples on methods of making and using embodiments of the invention. It is not intended to limit the scope.

Example 1

(S,Z)-5-(2-(3-aminopiperidin-1-yl)-3-chloro-5-(trifluoromethyl)benzylidene)thiazolidine-2,4-dione trifluoroacetate

A 40 mL vial was charged with a magnetic stir bar, 3-chloro-2-fluoro-5-(trifluoromethyl)benzaldehyde (0.134 ml, 1.10 mmol), acetonitrile (2.76 ml), (S)-tert-butyl piperidin-3-ylcarbamate (0.221 g, 1.10 mmol), and K₂CO₃ (0.229 g, 1.66 mmol). The vial was heated to 70° C. with stirring for 2 h. The vessel was cooled to rt and the mixture was diluted with DCM and filtered. The filtrate was conc. in vacuo to afford the substituted aldehyde which was dissolved in EtOH (2.76 ml). Thiazolidine-2,4-dione (0.155 g, 1.32 mmol) and piperidine (9.40 mg, 0.11 mmol) were then added. The mixture was heated to reflux for 4 h before being allowed to cool to rt and the mixture was conc. in vacuo. The product was dissolved DCM (2 mL) and TFA (1 mL) and stirred at rt for 1 h before being conc. in vacuo. The residue was dissolved in DMSO (˜2 mL) and purified via reverse phase HPLC to afford (S,Z)-5-(2-(3-aminopiperidin-1-yl)-3-chloro-5-(trifluoromethyl)benzylidene)thiazolidine-2,4-dione trifluoroacetate (0.214 g, 37.3%). ¹H NMR (300 MHz, DMSO-D6) δ ppm 12.78 (s, 1H) 7.95 (m, 3H) 7.79 (s, 1H) 7.62 (s, 1H) 3.40-3.20 (s, 5H) 2.12-2.06 (m, 1H) 1.79-1.70 (m 1H) 1.65-1.60 (m, 1H) 1.52-1.41 (m, 1H); m/z 406.

The following examples were prepared by the procedure of Example 1, using the appropriate starting materials. The following parent compounds obtained after chromatography may be converted to their corresponding hydrochloride salts using the procedure in Example 6, or a similar procedure.

Ex. Compound ¹H NMR (300 MHz) m/z SM 2  (R,Z)-5-(2-(3- 12.79 (s, 1 H) 7.98 (s, 2 406 (R)-tert-butyl piperidin- aminopiperidin-1-yl)- H) 7.95 (s, 1 H) 7.79 (s, 1 3-ylcarbamate 3-chloro-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione trifluoroacetate

H) 7.62 (s, 1 H) 3.31- 3.20 (m, 5 H) 2.12-2.09 (m, 1 H) 1.85-1.77 (m, 1 H) 1.66-1.63 (m, 1 H) 1.45-1.40 (m, 1 H)

3  (Z)-5-(2-(4- 12.76 (s, 1 H) 7.99 (s, 1 406 tert-butyl piperidin-4- aminopiperidin-1-yl)- H) 7.91 (s, 2 H) 7.78 (s, 1 ylcarbamate 3-chloro-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione trifluoroacetate

H) 7.60 (s, 1 H) 3.33- 3.18 (m, 5 H) 1.94 (d, 2 H) 1.71-1.60 (m, 2 H)

4  (Z)-5-(2-(3- 12.78 (s, 1 H) 7.93 (s, 1 421 tert-butyl piperidin-3- (aminomethyl) H) 7.84 (s, 1 H) 7.75 (s, 1 ylmethylcarbamate piperidin-1-yl)-3-chloro-5- (trifluoromethyl) benzylidene)thiazolidine- 2,4-dione trifluoroacetate

H) 7.61 (s, 1 H) 3.31- 3.05 (m, 6 H) 2.79-2.71 (m, 1 H) 1.95-1.89 (m, 2 H) 1.72-1.55 (m, 2 H) 1.29-1.10 (m, 1 H)

5A (Z)-5-(3-chloro-2- 12.83 (brs, 1 H) 9.20 405 tert-butyl 1,4-diazepane- (1,4-diazepan-1-yl)- (brs, 2 H) 8.05 (d, 1 H) 1-carboxylate 5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

7.90 (s, 1 H) 7.66 (d, 1 H) 3.51 (brs, 2 H) 3.35 (d, 2 H) 3.26-3.03 (m, 4 H) 2.27-1.99 (m, 2 H)

5B (S,Z)-5-(2-(3- 12.73 (brs, 1 H) 8.29 392 tert-butyl (3S)- aminopyrrolidin-1- (brs, 3 H) 7.93 (s, 1 H) pyrrolidin-3-ylcarbamate yl)-3-chloro-5-(trifluoromethyl) benzylidene)thiazolidine-2,4-dione

7.83 (s, 1 H) 7.66 (s, 1 H) 3.88 (brs, 1 H) 3.66 (dd, 1 H), 3.52-3.27 (m, 3 H) 2.42-2.19 (m, 1 H) 2.12- 1.88 (m, 1 H)

Example 6

(Z)-5-(3-chloro-2-(4-cyclopentylpiperazin-1-yl)-5-(trifluoromethyl)benzylidene)thiazolidine-2,4-dione hydrochloride

A 40 mL vial was charged with a magnetic stir bar, 3-chloro-2-fluoro-5-(trifluoromethyl)benzaldehyde (0.134 ml, 1.10 mmol), acetonitrile (2.76 ml), 1-cyclopentylpiperazine (0.213 g, 1.38 mmol), and K₂CO₃ (0.229 g, 1.66 mmol). The vial was heated to 70° C. with stirring for 2 h. The vessel was then cooled to rt and the mixture was diluted with DCM and filtered. The filtrate was conc. in vacuo to afford the substituted aldehyde which was dissolved in EtOH (2.76 ml). Thiazolidine-2,4-dione (0.155 g, 1.32 mmol) and piperidine (9.40 mg, 0.11 mmol) were then added and the mixture was heated to reflux for 4 h before being allowed to cool to rt. The mixture was then conc. in vacuo to afford the product which was dissolved in DMSO (˜2 mL) and purified via reverse phase HPLC to afford fractions that were conc. in vacuo, suspended in methanol (˜5 mL) and 1N HCl in diethyl ether (˜2 mL). This mixture was conc. in vacuo to afford (Z)-5-(3-chloro-2-(4-cyclopentylpiperazin-1-yl)-5-(trifluoromethyl)benzylidene)thiazolidine-2,4-dione hydrochloride (0.215 g, 39.3%). ¹H NMR (300 MHz, DMSO-D6) δ ppm 12.78 (s, 1H) 7.96 (s, 1H) 7.83 (s, 1H) 7.63 (s, 1H) 3.77-3.50 (m, 5H) 3.30-3.22 (m, 2H) 3.08-2.99 (m, 2H) 2.03-1.99 (m, 2H) 1.84-1.61 (m, 4H) 1.60-1.50 (m, 2H); m/z 461.

The following examples were prepared by the procedure of Example 6, using the appropriate starting materials. The following parent compounds obtained after chromatography may be converted to their corresponding hydrochloride salt in a manner similar as described in example 6.

Ex. Compound ¹H NMR m/z SM  7 (Z)-5-(3-chloro-2-(4- 12.74 (s, 1 H) 7.89-8.01 486 1-(4- (4-fluorophenyl)piperazin- (m, 2 H) 7.62 (s, 1 H) fluorophenyl)piperazine 1-yl)-5-(trifluoromethyl) benzylidene)thiazolidine-2,4-dione

6.99-7.13 (m, 4 H) 3.34- 3.23 (m, 8 H)

 8 (Z)-5-(2-(4-(benzo[d][1,3]dioxol- 12.77 (s, 1 H) 7.97 (d, 1 526 1-(benzo[d][1,3]dioxol- 5-ylmethyl)piperazin- H) 7.97 (s, 1 H) 7.77 (s, 1 5-ylmethyl)piperazine 1-yl)-3-chloro-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione trifluoroacetate

H) 7.63 (s, 1 H) 7.10 (s, 1 H) 7.02 (s, 2 H) 6.08 (s, 2 H) 4.34 (s, 2 H) 3.58- 3.11 (m, 8 H)

 9 (Z)-5-(3-chloro-2-(4-(3-methyl-1,2,4- 12.72 (s, 1 H) 7.91 (s, 1 473 3-methyl-5-(piperidin-4- oxadiazol-5-yl)piperidin-1-yl)-5- H) 7.84 (s, 1 H) 7.61 (s, 1 yl)-1,2,4-oxadiazole (trifluoromethyl)benzylidene) thiazolidine-2,4-dione

H) 3.33-3.18 (m, 4 H) 2.79-2.71 (m, 1 H) 2.33 (s, 3 H) 2.06 (d, 2 H) 1.98-1.80 (m, 2 H)

10 (Z)-5-(3-chloro-2-(4-(pyrrolidin-1- 12.78 (s, 1 H) 7.92 (s, 1 461 4-(pyrrolidin-1- yl)piperidin-1-yl)-5-(trifluoromethyl) H) 7.79 (s, 1 H) 7.60 (s, 1 yl)piperidine benzylidene)thiazolidine- 2,4-dione hydrochloride

H) 3.33-3.20 (m, 5 H) 3.12-3.03 (m, 4 H) 2.09 (d, 2 H) 1.97-1.78 (m, 6 H)

11 (Z)-5-(3-chloro-2-(4- 12.80 (s, 1 H) 7.96 (s, 1 434 1-isopropylpiperazine isopropylpiperazin-1- yl)-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

H) 7.82 (s, 1 H) 7.62 (s, 1 H) 3.85 (t, 2 H) 3.55-3.52 (m, 1 H) 3.41 (d, 2 H) 3.26 (d, 2 H) 3.10-3.06 (m, 2 H) 1.32 (d, 6 H)

12 (Z)-5-(3-chloro-2-(4- 12.80 (s, 1 H) 7.96 (d, 1 450 1-isobutylpiperazine isobutylpiperazin-1- yl)-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

H) 7.80 (s, 1 H) 7.63 (s, 1 H) 3.87 (brs, 2 H) 3.52 (d, 2 H) 3.27 (brs, 2 H) 3.11-3.00 (m, 4 H) 2.10 (qq, 1 H) 1.00 (d, 6 H)

13 (Z)-5-(3-chloro-2-(4- 12.73 (s, 1 H) 7.87 (s, 1 437 2-(piperidin-4-yl)ethanol (2-hydroxyethyl)piperidin- 1-yl)-5-(trifluoromethyl) benzylidene)thiazolidine-2,4-dione

H) 7.83 (s, 1 H) 7.59 (s, 1 H) 3.46 (t, 2 H) 3.23 (t, 2 H) 3.07 (brs, 2 H) 1.70 (d, 2 H) 1.61-1.52 (m, 1 H) 1.42 (q, 2 H) 1.33- 1.20 (m, 2 H)

14 (S,Z)-5-(3-chloro-2-(3-(dimethylamino) 12.65 (s, 1 H) 7.82 (s, 1 421 (S)-N,N- pyrrolidin-1-yl)-5-(trifluoromethyl) H) 7.72 (s, 1 H) 7.53 (s, 1 dimethylpyrrolidin-3- benzylidene)thiazolidine- H) 3.59-3.50 (m, 1 H) amine 2,4-dione hydrochloride

3.38-3.30 (m, 4 H) 2.56 (s, 6 H) 2.31-2.28 (m, 1 H) 2.09-2.01 (m, 1 H)

15 (Z)-5-(3-chloro-2-(4-(4-chloro-2- 12.75 (brs, 1 H) 8.06- 520 1-(4-chloro-2- fluorophenyl)piperazin-1-yl)-5- 7.90 (m, 2 H) 7.64 (d, 1 fluorophenyl)piperazine (trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

H) 7.38 (dd, 1 H) 7.23 (dd, 1 H) 7.11 (t, 1 H) 3.42 (m, 4 H) 3.15 (d, 4 H)

16 (Z)-5-(3-chloro-2-(3-(3-methyl-1,2,4- 471 3-methyl-5-(piperidin-3- oxadiazol-5-yl)piperidin-1-yl)-5- yl)-1,2,4-oxadiazole (trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

17 (Z)-5-(3-chloro-2-(4-(pyridin-4- 12.75 (brs, 1H) 8.78 (d, 2 483 1-(pyridin-4- ylmethyl)piperazin-1- H) 7.98 (s, 1 H) 7.74 ylmethyl)piperazine yl)-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

(brs, 1 H) 7.71 (s, 1 H) 7.64 (s, 1 H) 4.44 (brs, 2 H) 3.44 (brs, 4 H) 3.17 (s, 4 H)

18 (Z)-5-(3-chloro-2-(4-(2-chlorobenzyl) 7.98 (d, 1 H) 7.73 (dd, 1 517 1-(2- piperazin-1-yl)-5-(trifluoromethyl) H) 7.68-7.59 (m, 2 H) chlorobenzyl)piperazine benzylidene)thiazolidine- 2,4-dione hydrochloride

7.58-7.42 (m, 3 H) 4.53 (brs, 2 H) 3.75-3.30 (m, 8 H)

19 (Z)-5-(3-chloro-2-(4- 10.68 (brs, 1 H) 7.92 (d, 489 1-(1-methylpiperidin-4- (1-methylpiperidin-4- 1 H) 7.78 (brs, 1 H) 7.58 yl)piperazine yl)piperazin-1-yl)-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

(s, 1 H) 3.73-3.63 (m, 1 H) 3.61-3.41 (m, 6 H) 3.10 (brs, 3 H) 2.93 (brs, 3 H) 2.67 (s, 3 H) 2.39- 2.18 (m, 2 H) 2.07 (brs, 2 H)

20 (Z)-5-(3-chloro-2-(4-(2-morpholinoethyl) 11.12 (brs, 1 H) 7.91 (d, 505 4-(2-(piperazin-1- piperazin-1-yl)-5-(trifluoromethyl) 1 H) 7.79 (brs, 1 H) 7.59 yl)ethyl)morpholine benzylidene)thiazolidine- 2,4-dione hydrochloride

(s, 1 H) 3.80-2.81 (m, 20 H)

21 (Z)-5-(3-chloro-2-(4-(cyclopropylmethyl) 12.59 (brs, 1 H) 7.76 (d, 447 1-(cyclopropylmethyl) piperazin-1-yl)-5-(trifluoromethyl) 1 H) 7.61 (brs, 1 H) 7.43 piperazine benzylidene)thiazolidine- 2,4-dione hydrochloride

(s, 1 H) 3.62 (brs, 2 H) 3.44-3.24 (m, 2 H) 3.10 (brs, 2 H) 2.87 (d, 4 H) 0.94 (brs, 1 H) 0.54- 0.31 (m, 2 H) 0.32-0.08 (m, 2 H)

22 (Z)-5-(3-chloro-2-(4-morpholinopiperidin- 12.92 (brs, 1 H) 8.08 (d, 476 4-(piperidin-4- 1-yl)-5-(trifluoromethyl) 1 H) 7.95 (brs, 1 H) 7.76 yl)morpholine benzylidene)thiazolidine- 2,4-dione hydrochloride

(s, 1 H) 4.14 (brs, 3 H) 3.98 (brs, 2 H) 3.58 (brs, 3 H) 3.53-3.34 (m, 3 H) 3.28 (brs, 2 H) 2.32 (brs, 2 H) 1.92 (d, 2 H)

23 (Z)-5-(3-chloro-2-(4-(3-hydroxypropyl) 12.90 (brs, 1 H) 10.52 450 3-(piperazin-1-yl)propan- piperazin-1-yl)-5-(trifluoromethyl) (brs, 1 H) 7.98 (d, 1 H) 1-ol benzylidene)thiazolidine- 2,4-dione hydrochloride

7.84 (brs, 1 H) 7.65 (s, 1 H) 3.90-3.65 (m, 2 H) 3.57-3.41 (m, 5 H) 3.30-3.06 (m, 5 H) 1.96-1.73 (m, 2 H)

24 (Z)-5-(3-chloro-2-(4-(dimethylamino) 12.79 (brs, 1 H) 7.94 (d, 434 N,N-dimethylpiperidin-4- piperidin-1-yl)-5-(trifluoromethyl) 1 H) 7.82 (s, 1 H) 7.61 (s, amine benzylidene)thiazolidine- 2,4-dione hydrochloride

1 H) 3.84 (brs, 1 H) 3.46- 3.18 (m, 4 H) 2.75 (d, 6 H) 2.10 (d, 2 H) 1.74 (dd, 2 H)

25 (Z)-5-(3-chloro-2-((3-(dimethylamino) 12.81 (brs, 1 H) 7.98 (d, 422 N1,N1,N3- propyl)(methyl)amino)-5-(trifluoromethyl) 1 H) 7.81 (s, 1 H) 7.65 trimethylpropane-1,3- benzylidene)thiazolidine- (d, 1 H) 3.15 (t, 2 H) 3.02 diamine 2,4-dione hydrochloride

(brs, 2 H) 2.89 (s, 3 H) 2.72 (d, 6 H) 1.99-1.74 (m, 2 H)

26 (Z)-5-(3-chloro-2-(4-(2-hydroxyethyl)-1,4- 8.04 (brs, 1 H) 7.79 (d, 1 451 2-(1,4-diazepan-1- diazepan-1-yl)-5-(trifluoromethyl) H) 7.63 (s, 1 H) 3.83 (t, 3 yl)ethanol benzylidene)thiazolidine- 2,4-dione hydrochloride

H) 3.71 (m, 2 H) 3.56 (m, 3 H) 3.33 (m, 6 H) 2.21 (m, 2 H)

27 (Z)-5-(2-(4-butyl-1,4-diazepan-1-yl)-3- 10.76 (brs, 1 H) 8.06 (d, 463 1-butyl-1,4-diazepane chloro-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

1 H) 7.91 (brs, 1 H) 7.66 (d, 1 H) 3.69 (m, 3 H) 3.57 (brs, 1 H) 3.46 (m, 3 H) 3.14 (m, 3 H) 2.34 (brs, 1 H) 2.16 (brs, 1 H) 1.73 (d, 2 H) 1.32 (m, 2 H) 0.93 (t, 3 H)

28 (Z)-5-(3-chloro-2-(4-(2-hydroxyethyl) 7.98 (d, 1 H) 7.82 (brs, 1 436 2-(piperazin-1-yl)ethanol piperazin-1-yl)-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

H) 7.64 (d, 1 H) 5.40 (brs, 1 H) 3.74 (m, 4 H) 3.56 (m, 3 H) 3.27 (m, 3 H) 3.18 (brs, 1 H) 3.11 (brs, 1 H)

29 (Z)-5-(3-chloro-2-morpholino-5- 12.76 (brs, 1 H) 7.94 (m, 394 morpholine (trifluoromethyl) benzylidene)thiazolidine-2,4-dione

2 H) 7.63 (d, 1 H) 3.71 (t, 4 H) 3.20 (brs, 4 H)

30 (Z)-5-(2-(4-tert-butylpiperazin-1-yl)- 12.80 (brs, 1 H) 8.00 (d, 449 1-tert-butylpiperazine 3-chloro-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

1 H) 7.85 (brs, 1 H) 7.65 (d, 1 H) 3.82 (m, 2 H) 3.56 (d, 2 H) 3.29 (dd, 2 H) 3.04 (m, 2 H) 1.39 (s, 9 H)

31 (Z)-5-(2-(1,4′-bipiperidin-1′-yl)-3- 9.75 (brs, 1 H) 7.94 (d, 1 474 1,4′-bipiperidine chloro-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

H) 7.81 (brs, 1 H) 7.62 (s, 1 H) 3.29 (m, 7 H) 2.96 (m, 2 H) 2.14 (d, 2 H) 1.78 (m, 7 H) 1.44 (brs, 1 H)

32 (Z)-5-(3-chloro-2-(4-methylpiperazin-1- 7.94 (brs, 1 H) 7.75 406 1-methylpiperazine yl)-5-(trifluoromethyl) benzylidene)thiazolidine- 2,4-dione hydrochloride

(d, 1 H) 7.63 (s, 1 H) 3.56-3.38 (m, 3 H) 3.31- 3.21 (m, 5 H) 2.92 (s, 3 H)

Example 33

(S,Z)-5-(3-bromo-2-(3-(dimethylamino)pyrrolidin-1-yl)benzylidene)thiazolidine-2,4-dione hydrochloride

A 100 mL round bottom flask was charged with a magnetic stir bar, (S)-3-bromo-2-(3-(dimethylamino)pyrrolidin-1-yl)benzaldehyde (Method 1) (0.370 g, 1.24 mmol), thiazolidine-2,4-dione (0.146 g, 1.24 mmol), and ethanol (4.15 ml). Piperidine (0.012 ml, 0.12 mmol) was then added and the reaction was heated to reflux for 2 h. Once the reaction was judged to be complete by LCMS, it was allowed to cool to rt and was conc. in vacuo to afford the product which was purified on 40 g of silica gel using ethyl acetate/methanol (3:1) as eluent to afford the free base which was suspended in methanol (˜5 mL) and 1N HCl in diethyl ether (2 mL) and conc. in vacuo to afford (S,Z)-5-(3-bromo-2-(3-(dimethylamino)pyrrolidin-1-yl)benzylidene)thiazolidine-2,4-dione hydrochloride (0.141 g, 26.2%). ¹H NMR (300 MHz, DMSO-D6) δ ppm 12.68 (s, 1H) 7.91 (s, 1H) 7.77 (d, 1H) 7.48 (d, 1H) 7.33 (t, 1H) 4.10-4.02 (m, 1H) 3.62 (t, 2H) 3.50-3.45 (m, 2H) 2.81 (s, 6H) 2.42-2.38 (m, 1H) 2.31-2.21 (m, 1H); m/z 398.

The following examples were prepared by the procedure of Example 33, using the appropriate starting materials. The following parent compounds obtained after chromatography (normal or reverse phase) may be converted to its corresponding hydrochloride salt as described in example 33, or similar procedure.

Ex. Compound ¹H NMR m/z SM 34 (Z)-5-(2-((3S,5R)-3,5- dimethylpiperazin-1- yl)-5- (trifluoromethyl) benzylidene) thiazolidine- 2,4-dione hydrochloride  

12.63 (s, 1 H) 8.99-8.94 (m, 1 H) 7.77 (d, 1 H) 7.72 (s, 2 H) 7.40 (d, 1 H) 3.49-3.43 (m, 2 H) 3.32 (d, 2 H) 2.92 (t, 2 H) 1.25 (d, 6 H) 387 2-((3S,5R)-3,5- dimethylpiperazin-1-yl)- 5-(trifluoromethyl) benzaldehyde Method 2  

35 (Z)-5-(2-((3S,5R)-3,5- dimethylpiperazin-1- yl)-3- methoxybenzylidene) thiazolidine-2,4- dione  

7.87 (brs, 1 H) 7.24 (t, 1 H) 7.20-7.10 (m, 1 H) 7.02 (d, 1 H) 3.81 (s, 3 H) 3.48-3.23 (m, 2 H) 3.17 (t, 2 H) 2.89 (d, 2 H) 1.30-1.09 (m, 6 H) 348 2-((3S,5R)-3,5- dimethylpiperazin-1-yl)- 3-methoxybenzaldehyde Method 3  

36 (S,Z)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-3- methoxybenzylidene) thiazolidine-2,4- dione  

7.89 (s, 1 H) 7.26 (m, 1 H) 7.10 (m, 2 H) 3.84 (s, 3 H) 3.41-3.29 (m, 7 H) 3.24-3.13 (m, 4 H) 2.18 (m, 1 H) 1.99 (m, 1 H) 348 (S)-2-(3- (dimethylamino) pyrrolidin-1-yl)-3- methoxybenzaldehyde Method 4  

37 (Z)-5-(3-chloro-2- ((3S,5R)-3,5- dimethylpiperazin-1- yl)benzylidene) thiazolidine-2,4-dione  

7.80 (s, 1 H) 7.50 (brs, 1 H) 7.38 (d, 1 H) 7.30 (brs, 1 H) 3.34-3.02 (m, 6 H) 1.26-1.09 (m, 6 H) 352 3-chloro-2-((3S,5R)-3,5- dimethylpiperazin-1- yl)benzaldehyde Method 5  

38 (S,Z)-5-(3-chloro-2- (3- (dimethylamino) pyrrolidin-1- yl)benzylidene) thiazolidine-2,4-dione  

7.73 (s, 1 H) 7.52-7.47 (m, 2 H) 7.47 (s, 1 H) 7.33-7.31 (m, 1 H) 3.58- 3.52 (m, 1 H) 3.48 (m, 1 H) 3.37-3.27 (m, 3 H) 2.56 (s, 6 H) 2.26-2.35 (m, 1 H) 2.08 (m, 1 H) 352 (S)-3-chloro-2-(3- (dimethylamino) pyrrolidin-1-yl) benzaldehyde Method 6  

39 (R,Z)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-4,5- dimethoxybenzylidene) thiazolidine-2,4- dione  

7.75 (s, 1 H) 6.98 (s, 1 H) 6.68 (s, 1 H) 3.83 (s, 3 H) 3.72 (s, 3 H) 3.24-3.06 (m, 4 H) 2.50 (m, 1 H) 2.32 (s, 6 H) 2.12 (m, 1 H) 1.84 (m, 1 H) 378 (R)-2-(3- (dimethylamino) pyrrolidin-1-yl)-4,5- dimethoxybenzaldehyde Method 7  

40 (S,Z)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-4,5- dimethoxybenzylidene) thiazolidine-2,4- dione  

7.76 (s, 1 H) 6.98 (s, 1 H) 6.68 (s, 1 H) 3.83 (s, 3 H) 3.72 (s, 3 H) 3.26-3.01 (m, 5 H) 2.32 (s, 6 H) 2.15-2.09 (m, 1 H) 1.90- 1.81 (m, 1 H) 378 (S)-2-(3- (dimethylamino) pyrrolidin-1-yl)-4,5- dimethoxybenzaldehyde Method 8  

41 (5Z)-5-{2-[4- (hydroxymethyl) piperidin-1-yl]-4,5- dimethoxybenzylidene}- 1-,3-thiazolidine- 2,4-dione  

12.44 (brs, 1 H) 7.94 (s, 1 H) 7.05-6.87 (m, 1 H) 6.80 (s, 1 H) 4.54 (t, 1 H) 3.85 (s, 3H) 3.76 (s, 3 H) 3.17 (d, 1 H) 3.03 (d, 2 H) 2.71 (t, 2 H) 1.75 (brs, 2 H) 1.56-1.26 (m, 3 H) 379 2-(4- (hydroxymethyl) piperidin-1-yl)-4,5- dimethoxybenzaldehyde Method 9  

42 (5Z)-5-[2-(4- hydroxypiperidin-1- dimethoxybenzylidene]- 1,3-thiazolidine- 2,4-dione  

12.50 (brs, 1 H) 8.02 (s, 1 H) 6.99 (s, 1 H) 6.92- 6.82 (m, 1 H) 4.79 (d, 1 H) 3.90 (s, 3 H) 3.82 (s, 3 H) 3.74-3.58 (m, 1 H) 3.13-2.96 (m, 2 H) 2.88- 2.71 (m, 2 H) 2.01- 1.81 (m, 2 H) 1.78-1.58 (m, 2 H) 365 2-(4-hydroxypiperidin-1- yl)-4,5- dimethoxybenzaldehyde Method 10  

43 (5Z)-5-{2-[3- (dimethylamino) pyrrolidin-1-yl]-5- methoxybenzylidene]- 1,3-thiazolidine-2,4- dione  

7.51 (s, 1 H) 7.03 (d, 1 H) 6.99 (d, 1 H) 6.84 (dd, 1 H) 3.72 (s, 3 H) 3.09- 3.21 (m, 1 H) 2.82-3.09 (m, 4 H) 2.23 (s, 6 H) 2.06 (d, 1 H) 1.73-1.85 (m, 1 H) 348 2-(3- (dimethylamino) pyrrolidin-1-yl)-5- methoxybenzaldehyde Method 11  

44 (5Z)-5-{2-[4- (dimethylamino) piperidin-1-yl]-5- methoxybenzylidene] 1,3-thiazolidine-2,4- dione  

7.59 (s, 1 H) 7.11-6.99 (m, 2 H) 6.85 (dd, 1 H) 3.75 (s, 3 H) 3.03 (m, 2 H) 2.64-2.54 (m, 2 H) 2.44-2.33 (m, 7 H) 1.91 (s, 2 H) 1.62 (dd, 2 H) 362 2-(4- (dimethylamino)piperidin- 1-yl)-5- methoxybenzaldehyde Method 12  

45 (5Z)-5-[2-(3- hydroxypyrrolidin-1- yl)-4,5- dimethoxybenzylidene]- 1,3-thiazolidine- 2,4-dione  

12.32 (brs, 1 H) 7.88 (s, 1 H) 6.93 (s, 1 H) 6.66 (s, 1 H) 4.34 (brs, 1 H) 3.83 (s, 3 H) 3.72 (s, 3 H) 3.55- 3.29 (m, 3 H) 3.22 (brs, 1 H) 2.90 (d, 1 H) 2.16- 1.94 (m, 1 H) 1.84 (d, 1 H) 351 2-(3-hydroxypyrrolidin- 1-yl)-4,5- dimethoxybenzaldehyde Method 13  

46 (5Z)-5-(4,5- dimethoxy-2- pyrrolidin-1- ylbenzylidene)-1,3- thiazolidine-2,4- dione  

12.21 (brs, 1 H) 7.86 (s, 1 H) 6.94 (s, 1 H) 6.65 (s, 1 H) 3.83 (s, 3 H) 3.72 (s,3 H) 3.12 (brs, 4 H) 1.90 (brs, 4 H) 335 4,5-dimethoxy-2- (pyrrolidin-1- yl)benzaldehyde Method 14  

47 (5Z)-5-{2-[[2- (dimethylamino)ethyl] (methyl)amino] benzylidene}-1,3- thiazolidine-2,4- dione  

7.62 (s, 1 H) 7.48 (d, 1 H) 7.32 (t, 1 H) 7.17 (d, 1 H) 7.10 (t, 1 H) 3.06 (t, 2 H) 2.86-2.62 (m, 5 H) 2.36 (s, 6 H) 306 2-((2- (dimethylamino)ethyl) (methyl)amino) benzaldehyde Method 15  

48 (5Z)-5-{2-[3- (dimethylamino) pyrrolidin-1- yl]benzylidene}-1,3- thiazolidine-2,4- dione  

7.52 (s, 1 H) 7.27-7.21 (m, 1 H) 7.14-7.05 (m, 1 H) 6.84 (d, 1 H) 6.77 (t, 1 H) 3.17 (s, 1 H) 3.16- 3.09 (m, 1 H) 3.06-2.94 (m, 2 H) 2.86-2.77 (m, 1 H) 2.16-2.11 (m, 6 H) 2.03-1.92 (m, 1 H) 1.66 (dd, 1 H) 318 2-(3- (dimethylamino)pyrrolidin- 1-yl)benzaldehyde Method 16  

49 (5Z)-5-{2-[4- (dimethylamino) piperidin-1- yl]benzylidene}-1,3- thiazolidine-2,4- dione  

7.64 (s, 1 H) 7.50 (d, 1 H) 7.35-7.27 (m, 1 H) 7.12 (t, 2 H) 3.22 (m, 2 H) 2.88 (m, 1 H) 2.68 (t, 2 H) 2.63 (s, 6 H) 2.04 (s, 2 H) 1.73 (td, 2 H) 332 2-(4- (dimethylamino)piperidin- 1-yl)benzaldehyde Method 17  

50 (5Z)-5-[2-(4- isopropylpiperazin-1- yl)benzylidene]-1,3- thiazolidine-2,4- dione  

7.80 (s, 1 H) 7.49-7.43 (m, 1 H) 7.43-7.39 (m, 1 H) 7.22-7.13 (m, 2 H) 2.99-2.88 (m, 5 H) 2.80 (s, 4 H) 1.13-1.04 (m, 6 H) 332 2-(4-isopropylpiperazin- 1-yl)benzaldehyde Method 18  

51 (5Z)-5-{2-[[2- (dimethylamino)ethyl] (methyl)amino]-4,5- dimethoxybenzylidene}- 1,3-thiazolidine- 2,4-dione  

7.78 (s, 1 H) 7.02 (s, 1 H) 6.85 (s, 1 H) 3.83 (s, 3 H) 3.76 (s, 3 H) 3.11-2.98 (m, 2 H) 2.76-2.65 (m, 5 H) 2.37 (s, 6 H) 364 2-((2- (dimethylamino)ethyl) (methyl)amino)-4,5- dimethoxybenzaldehyde Method 19  

52 (5Z)-5-{2-[3- (dimethylamino) pyrrolidin-1-yl]-4,5- dimethoxybenzylidone- 1,3-thiazolidine- 2,4-dione  

7.76 (s, 1 H), 6.98 (s, 1 H), 6.68 (s, 1 H), 3.83 (s, 3 H), 3.72 (s, 3 H), 3.25 (m, 1 H), 3.13 (m, 2 H), 3.06 (m, 2 H), 2.32 (s, 6 H), 2.15-2.09 (m, 1 H) 1.90-1.81 (m, 1 H) 378 2-(3- (dimethylamino) pyrrolidin-1-yl)-4,5- dimethoxybenzaldehyde Method 20  

53 (5Z)-5-{2-(4- isopropylpiperazin-1- yl)-4,5- dimethoxybenzylidene}- thiazolidine-2,4- dione  

7.86 (s, 1 H) 6.95 (s, 1 H) 6.77 (s, 1 H) 3.81 (s, 3 H) 3.76-3.67 (m, 3 H) 2.91- 2.79 (m, 5 H) 2.71 (s, 4 H) 1.08-1.00 (m, 6 H) 392 2-(4-isopropylpiperazin- 1-yl)-4,5- dimethoxybenzaldehyde Method 21  

54 (5Z)-5-{2-(4- (dimethylamino) piperidin-1-yl)-4,5- dimethoxybenzylidene}- thiazolidine-2,4- dione  

7.48 (s, 1 H) 6.82 (s, 1 H) 6.51 (s, 1 H) 3.58 (s, 3 H) 3.51 (s, 3 H) 2.87 (d, 2 H) 2.47 (m, 3 H) 2.35 (s, 6 H) 1.79 (d, 2 H) 1.49 (d, 2 H) 392 2-(4- (dimethylamino)piperidin- 1-yl)-4,5- dimethoxybenzaldehyde Method 22  

55 (Z)-tert-butyl 4-(2- ((2,4- dioxothiazolidin-5- ylidene)methyl)-4- (trifluoromethyl) phenyl)piperazine-1- carboxylate  

12.65 (s, 1 H) 7.82 (s, 1 H) 7.72 (s, 1 H) 7.53 (s, 1 H) 3.59-3.50 (m, 1 H) 3.38-3.30 (m, 4 H) 2.56 (s, 6 H) 2.31-2.28 (m, 1 H) 2.09-2.01 (m, 1 H) 458 tert-butyl 4-(2-formyl-4- (trifluoromethyl)phenyl) piperazine-1-carboxylate Method 23  

56 (Z)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-5- nitrobenzylidene) thiazolidine-2,4-dione hydrochloride  

12.64 (s, 1 H) 8.22 (d, 1 H) 8.12 (d, 1 H) 7.95 (d, 1 H) 7.04 (d, 1 H) 3.95- 3.90 (m, 1 H) 3.70-3.65 (m, 2 H) 3.56-3.51 (m, 2 H) 2.79 (s, 6 H) 2.41- 2.35 (m, 1 H) 2.34-2.22 (m, 1 H) 363 2-(3- (dimethylamino) pyrrolidin-1-yl)-5- nitrobenzaldehyde Method 24  

57 (Z)-4-(3- (dimethylamino) pyrrolidin-1-yl)-3-((2,4- dioxothiazolidin-5- ylidene)methyl) benzamide hydrochloride  

12.47 (s, 1 H) 7.92-7.84 (m, 4 H) 7.23 (s, 1 H) 7.03 (d, 1 H) 3.98-3.91 (m, 1 H) 3.53-3.49 (m, 2 H) 3.46-3.39 (m, 2 H) 2.79 (s, 6 H) 2.34-2.19 (m, 2 H) 361 4-(3- (dimethylamino) pyrrolidin-1-yl)-3- formylbenzamide Method 25  

58 (Z)-tert-butyl 4-(2- ((2,4- dioxothiazolidin-5- ylidene)methyl) phenyl)piperazine-1- carboxylate  

12.55 (s, 1 H) 7.92 (s, 1 H) 7.46-7.44 (m, 2 H) 7.21-7.18 (m, 2 H) 3.48 (brs, 4 H) 2.86 (brs, 4 H) 1.43 (s, 9 H) 389 tert-butyl 4-(2- formylphenyl)piperazine- 1-carboxylate Method 26  

59 (Z)-5-(3-methoxy-4- (2-(piperidin-1- yl)ethoxy)benzylidene) thiazolidine-2,4- dione  

7.78 (s, 1 H) 7.27 (s, 1 H) 7.21 (s, 2 H) 4.47 (t, 2 H) 3.84 (s, 3 H) 3.49 (t, 4 H) 3.04 (brs, 2H) 1.80 (d, 4 H) 1.64 (brs, 1 H) 1.43 (brs, 1 H) 363 3-methoxy-4-(2- (piperidin-1- yl)ethoxy)benzaldehyde Commercial  

60 (Z)-5-(2-(2- hydroxyethoxy) benzylidene) thiazolidine- 2,4-dione  

12.58 (brs, 1 H) 8.06 (s, 1 H) 7.57-7.32 (m, 2 H) 7.26-7.03 (m, 2 H) 4.95 (brs, 1 H) 4.13 (t, 2 H) 3.77 (brs, 2 H) 266 2-(2- hydroxyethoxy) benzaldehyde Commercial  

61 (Z)-5-(5-methoxy-2- (2-(piperidin-1- yl)ethoxy) benzylidene) thiazolidine-2,4- dione  

7.80 (s, 1 H) 7.10 (d, 1 H) 7.04-6.95 (m, 2 H) 4.22 (t, 2 H) 3.75 (s, 3 H) 3.07 (t, 2 H) 2.84 (s, 4 H) 1.62 (dq, 4 H) 1.46 (d, 2 H) 363 5-methoxy-2-(2- (piperidin-1- yl)ethoxy)benzaldehyde Method 27  

62 (Z)-5-(5-methoxy-2- (2- morpholinoethoxy) benzylidene) thiazolidine-2,4-dione  

7.99 (s, 1 H) 7.22-7.10 (m, 1 H) 7.09-6.97 (m, 1 H) 6.91 (d, 1 H) 4.17 (t, 2 H) 3.76 (s, 3 H) 3.64- 3.51 (m, 4 H) 2.75 (t, 2 H) 2.51-2.62 (m, 4 H) 365 5-methoxy-2-(2- morpholinoethoxy) benzaldehyde Method 28  

63 (Z)-5-(2-(2- (diethylamino)ethoxy)- 5-methoxybenzylidene) thiazolidine-2,4- dione  

7.77 (s, 1 H) 7.08-7.05 (m, 1 H) 7.03-6.95 (m, 2 H) 4.19 (t, 2 H) 3.75 (s, 3 H) 3.25-3.14 (m, 2 H) 2.95 (q, 4 H) 1.17-1.08 (m, 6 H) 352 2-(2- (diethylamino)ethoxy)-5 methoxybenzaldehyde Method 29  

64 (Z)-5-(2-(2- (diethylamino) ethoxy)benzylidene) thiazolidine-2,4-dione  

7.84 (s, 1 H) 7.47 (d, 1 H) 7.43-7.35 (m, 1 H) 7.14-7.04 (m, 2 H) 4.24 (t, 2 H) 3.20 (m, 2 H) 2.90 (q, 4 H) 1.13 (t, 6 H) 321 2-(2- (diethylamino)ethoxy) benzaldehyde Method 30  

65 (Z)-5-(4,5- dimethoxy-2- (pyridin-3- yl)benzylidene) thiazolidine-2,4-dione  

12.55 (brs, 1 H) 8.73- 8.61 (m, 1 H) 8.58 (brs, 1 H) 7.81 (dd, 1 H) 7.53 (dd, 1 H) 7.46 (s, 1 H) 7.13 (d, 2 H) 3.89 (d, 6 H) 343 4,5-dimethoxy-2- (pyridin-3- yl)benzaldehyde Method 31  

66 (Z)-5-(4,5- dimethoxy-2- (pyridin-4- yl)benzylidene) thiazolidine-2,4-dione  

12.58 (brs, 1 H) 8.67 (d, 2 H) 7.48 (d, 1 H) 7.41 (d, 2 H) 7.12 (d, 2 H) 3.89 (s, 6 H) 343 4,5-dimethoxy-2- (pyridin-4- yl)benzaldehyde Method 32  

67 (Z)-5-((1H-indol-3- yl)methylene) thiazolidine-2,4-dione  

7.21 (dddd, 2 H) 7.50 (d, 1 H) 7.71 (d, 1 H) 7.87 (d, 1 H) 7.99 (s, 1 H) 12.07 (brs, 2 H) 245 1H-indole-3- carbaldehyde Commercial  

68 (Z)-5-((1H-indazol-3- yl)methylene) thiazolidine-2,4-dione  

7.28 (t, 1 H) 7.52-7.39 (m, 1 H) 7.64 (d, 1 H) 8.22-8.03 (m, 2 H) 12.41 (d, 1 H) 13.96 (s, 1 H) 246 1H-indazole-3- carbaldehyde Commercial  

69 (Z)-5-((6-oxo-1,6- dihydropyridin-3- yl)methylene) thiazolidine-2,4-dione  

11.99 (brs, 1 H) 7.74 (brs, 1 H) 7.69-7.58 (m, 1 H) 7.27 (s, 1 H) 6.43 (d, 1 H) 223 6-oxo-1,6- dihydropyridine-3- carbaldehyde Commercial  

70 (Z)-5-((2-oxo-1,2- dihydropyridin-3- yl)methylene) thiazolidine-2,4-dione  

12.43 (brs, 1 H) 12.16 (brs, 1 H) 7.73 (s, 1 H) 7.69 (dd, 1 H) 7.57 (d, 1 H) 6.38 (t, 1 H) 223 2-oxo-1,2- dihydropyridine-3- carbaldehyde Commercial  

71 (Z)-5-((1H-pyrazol-4- yl)methylene) thiazolidine-2,4-dione  

13.51 (brs, 1 H) 12.37 (brs, 1 H) 8.20 (s, 1 H) 7.82 (s, 1 H) 7.74 (s, 1 H) 196 1H-pyrazole-4- carbaldehyde Commercial  

72 (Z)-5-(pyridin-4- ylmethylene) thiazolidine-2,4-dione  

12.61 (brs, 1 H) 8.71 (d, 2 H) 7.74 (s, 1 H) 7.53 (d, 2 H) 207 4- pyridinecarboxaldehyde Commercial  

73 (5Z)-5-[(1-methyl- 1H-1,2,3- benzotriazol-5- yl)methylene]-1,3- thiazolidine-2,4- dione  

12.65 (brs, 1 H) 8.28 (s, 1 H) 8.00-7.98 (m, 2 H) 7.76 (d, 1 H) 4.33 (s, 3 H) 259 1-methyl-1H- benzo[d][1,2,3]triazole- 5-carbaldehyde Commercial  

74 (5Z)-5-(3,4- dimethoxybenzylidene)- 1,3-thiazolidine- 2,4-dione  

12.51 (brs, 1 H) 7.74 (s, 1 H) 7.19-7.09 (m, 3 H) 3.82 (s, 3 H) 3.80 (s, 3 H) 266 3,4- dimethoxybenzaldehyde Commercial  

75 (5Z)-5-[(1-methyl- 1H-indol-6- yl)methylene]-1,3- thiazolidine-2,4- dione  

12.49 (brs, 1 H) 7.92 (s, 1 H) 7.68 (d, 1 H) 7.54 (s 1 H) 7.25 (d, 1 H) 6.50 (s, 1 H) 3.84 (s, 3 H) 1-methyl-1H-indole-6- carbaldehyde Commercial  

76 (5Z)-5-[(1-methyl- 1H-indol-5- yl)methylene]-1,3- thiazolidine-2,4- dione  

12.45 (brs, 1 H) 7.90 (s, 1 H) 7.84 (d, 1 H) 7.59 (d, 1 H) 7.44 (d, 1 H) 7.38 (d 1 H) 6.57 (d, 1 H) 3.82 (s, 3 H) 259 1-methyl-1H-indole-5- carbaldehyde Commercial  

77 (5Z)-5-(quinolin-6- ylmethylene)-1,3- thiazolidine-2,4- dione trifluoroacetate  

12.70 (brs, 1 H) 8.97 (d, 1 H) 8.51 (d, 1 H) 8.24 (s, 1 H) 8.12 (d, 1 H) 7.96-7.93 (m, 2 H) 7.64- 7.61 (m, 1 H) 257 quinoline-6-carbaldehyde Commercial  

78 (5Z)-5-(1H-indol-5- ylmethylene)-1,3- thiazolidine-2,4- dione  

12.43 (brs, 1 H) 11.44 (brs, 1 H) 7.85 (d, 1 H) 7.53-7.51 (m, 2 H) 7.45- 7.44 (m, 1 H) 7.33 (d, 1 H) 6.56 (s, 1 H) 245 1H-indole-5- carbaldehyde Commercial  

79 (5Z)-5-(1H-indol-6- ylmethylene)-1,3- thiazolidine-2,4- dione trifluoroacetate  

12.47 (brs, 1 H) 11.48 (brs, 1 H) 7.90 (s, 1 H) 7.69-7.66 (m, 2 H) 7.55- 7.52 (m, 1 H) 7.23 (d, 1 H) 6.51 (s, 1 H) 245 1H-indole-6- carbaldehyde Commercial  

80 (5Z)-5-(1H- pyrrolo[2,3- b]pyridin-5- ylmethylene)-1,3- thiazolidine-2,4- dione  

12.02 (brs, 1 H) 8.49 (d, 1 H) 8.15 (d, 1 H) 7.92 (s, 1 H) 7.58 (t, 1 H) 6.59 (brs, 1 H) 246 1H-pyrrolo[2,3- b]pyridine-5- carbaldehyde Commercial  

81 (5Z)-5-{2-[3- (dimethylamino) propoxy]benzylidene}- 1,3-thiazolidine-2,4- dione  

8.16 (s, 1 H) 7.76 (s, 1 H) 7.48 (d, 1 H) 7.34 (s, 1 H) 7.08 (d, 1 H) 4.11 (t, 2 H) 2.86 (1, 2 H) 2.53 (s, 6 H) 2.10-1.98 (m, 2 H) 307 2-[3- (dimethylamino) propoxy]benzaldehyde Commercial  

82 (5Z)-5-(2-morpholin- 4-ylbenzylidene)-1,3- thiazolidine-2,4- dione  

7.89 (s, 1 H) 7.46 (d, 1 H) 7.45-7.41 (m, 1 H) 7.22-7.14 (m, 2 H) 3.80- 3.69 (m, 4 H) 2.93- 2.82 (m, 4 H) 291 2-morpholin-4- ylbenzaldehyde Commercial  

83 (5Z)-5-(3-morpholin- 4-ylbenzylidene)-1,3- thiazolidine-2,4- dione trifluoroacetate  

12.58 (brs, 1 H) 7.74 (s, 1 H) 7.37 (t, 1 H) 7.12 (s, 1 H) 7.07 (dd, 1 H) 7.00 (d, 1 H) 3.79-3.69 (m, 4 H) 3.20-3.09 (m, 4 H) 290 3-morpholin-4- ylbenzaldehyde Commercial  

84A (5Z)-5-[2-(4- methylpiperazin-1- yl)benzylidene]-1,3- thiazolidine-2,4- dione  

7.78 (s, 1 H) 7.46 (d, 1 H) 7.42-7.35 (m, 1 H) 7.19-7.11 (m, 2 H) 2.94 (t, 4 H) 2.70 (brs, 4 H) 2.40 (s, 3 H) 304 2-(4-methylpiperazin-1- yl)benzaldehyde Commercial  

84B (Z)-5-(3-(3-(4- methylpiperazin-1- yl)propoxy)benzylidene) thiazolidine-2,4- dione  

11.37 (brs, 1 H) 7.54 (s, 1 H) 7.38 (t, 1 H) 7.12 (m, 2 H), 6.96 (m, 1 H) 4.58 (s, 3 H) 4.05 (t, 2 H) 3.00- 2.55 (m, 10 H) 1.93 (m, 2 H) 362 3-(3-(4-methylpiperazin- 1- yl)propoxy)benzaldehyde hydrochloride  

84C (Z)-2-(3-((2,4- dioxothiazolidin-5- ylidene)methyl) phenoxy)acetamide  

12.62 (s, 1 H) 7.73 (s, 1 H) 7.60 (s, 1 H) 7.44 (t, 1 H) 7.41 (s, 1 H) 7.19 (d, 1 H) 7.14 (m, 1 H) 7.05 (dd, 1 H) 4.48 (s, 2 H) 279 2-(3- formylphenoxy)acetamide  

84D (Z)-5-(3-(3- (piperidin-1- yl)propoxy)benzylidene) thiazolidine-2,4- dione  

7.30 (t, 1 H) 7.24 (s, 1 H) 7.08 (m, 2 H) 6.85 (dd, 1 H) 4.01 (t, 2 H) 2.45 (t, 2 H) 2.40 (m, 4 H) 1.88 (m, 2H) 1.50 (m, 4 H) 1.38 (m, 2 H) 347 3-(3-(piperidin-1- yl)propoxy)bcnzaldehyde hydrochloride  

84E (Z)-5-(3-((4- methylpiperazin-1- yl)methyl)benzylidene) thiazolidine-2,4- dione  

7.59 (s, 1 H) 7.51 (s, 1 H) 7.46 (m, 2 H) 7.34 (m, 1 H) 3.59 (s, 2 H) 2.96 (m, 4 H) 2.59 (m, 4 H) 2.58 (s, 3 H) 318 3-((4-methylpiperazin-1- yl)methyl)benzaldehyde  

84F (Z)-N-(2- (dimethylamino) ethyl)-2′-((2,4- dioxothiazolidin-5- ylidene)methyl)-N- methylbiphenyl-4- sulfonamide  

7.90 (d, 2 H) 7.69 (d, 1 H) 7.65-7.49 (m, 4 H) 7.49-7.40 (m, 1 H) 7.31 (s, 1 H) 3.24 (t, 2 H) 2.83 (t, 2 H) 2.78 (s, 3 H) 2.48 (s, 6 H) 445 N-(2- (dimethylamino)ethyl)- 2′-formyl-N- methylbiphenyl-4- sulfonamide Method 32B  

84G (S)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-5- methoxybenzylidene) thiazolidine-2,4- dione  

348 (S)-2-(3- (dimethylamino) pyrrolidin-1-yl)-5- methoxybenzaldehyde Method 104  

84H (S)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-5- (trifluoromethyl) benzylidene)thiazolidine- 2,4-dione  

12.55 (s, 1 H) 11.25 (brs, 1 H) 7.95 (s, 1 H) 7.78 (s, 1 H) 7.60 (m, 1 H), 7.15 (m, 1 H) 4.00 (m, 1 H) 3.65 (m, 2 H) 3.40 (m, 1 H) 3.32 (m, 1 H) 2.80 (s, 6 H) 2.35 (m, 1 H) 2.20 (m, 1 H) 386 (S)-2-(3- (dimethylamino) pyrrolidin-1-yl)-5- (trifluoromethyl) benzaldehyde Method 105  

84I (S)-5-(5-chloro-2-(3- (dimethylamino) pyrrolidin-1- yl)benzylidene) thiazolidine-2,4-dione  

12.55 (s, 1 H) 7.81 (s, 1H), 7.93 (m, 2H), 7.12 (m, 1H), 3.90 (m, 1H), 3.45-3.30 (m, 2H), 3.29 (m, 1H), 3.15 (m, 1H), 2.80 (s, 6H), 2.35 (m, 1H), 2.20 (m, 1H) 352 (S)-5-chloro-2-(3- (dimcthylamino) pyrrolidin-1- yl)benzaldehyde Method 106  

84J (S)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-4- methylbenzylidene) thiazolidine-2,4-dione  

332 (S)-2-(3- (dimethylamino) pyrrolidin-1-yl)-4- methylbenzaldehyde Method 107  

84K (S)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-5- fluorobenzylidene) thiazolidine-2,4-dione  

12.55 (s, 1 H) 11.40 (s, 1 H) 7.80 (s, 1 H) 7.30- 7.15 (m, 3 H) 3.92 (m, 1 H) 3.45 (m, 1 H) 3.35 (m, 1 H) 3.18 (m, 1 H) 3.15 (m, 1 H) 3.80 (d, 6 H) 2.30 (m, 1 H), 2.20 (m, 1H) 336 (S)-2-(3- (dimethylamino) pyrrolidin-1-yl)-5- fluorobenzaldehyde Method 108  

84L (S)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-5- methylbenzylidene) thiazolidine-2,4-dione  

332 (S)-2-(3- (dimethylamino) pyrrolidin-1-yl)-5- methylbenzaldehyde Method 109  

84M (S)-5-(5-bromo-2-(3- (dimethylamino) pyrrolidin-1- yl)benzylidene) thiazolidine-2,4-dione  

397 (S)-5-bromo-2-(3- (dimethylamino) pyrrolidin-1-yl) benzaldehyde Method 110  

84N (S)-5-(2-(3- (dimethylamino) pyrrolidin-1-yl)-3- fluorobenzylidene) thiazolidine-2,4-dione  

12.55 (s, 1 H) 10.34 (s, 1 H) 7.87 (d, 1H) 7.33-7.21 (m, 3 H) 3.93 (m, 1 H) 3.40 (m, 2 H) 3.25 (m, 1 H) 3.18 (m, 1 H) 2.79 (s, 6 H) 2.26 (m, 1H) 2.12 (m, 1H) 336 (S)-2-(3- (dimethylamino) pyrrolidin-1-yl)-3- fluorobenzaldehyde Method 111  

84O (S)-5-(2-chloro-6-(3- (dimethylamino) pyrrolidin-1- yl)benzylidene) thiazolidine-2,4-dione  

352 (S)-2-chloro-6-(3- (dimethylamino) pyrrolidin-1-yl) benzaldehyde Method 112  

Example 85

(S,Z)-5-(2-(3-aminopyrrolidin-1-yl)-4-chlorobenzylidene)thiazolidine-2,4-dione hydrochloride

A 100 mL round bottom flask was charged with a magnetic stir bar, (S,Z)-tert-butyl 1-(5-chloro-2-((2,4-dioxothiazolidin-5-ylidene)methyl)phenyl)pyrrolidin-3-ylcarbamate (Method 33) (1.300 g, 3.07 mmol), MeOH (10.22 ml), and a 1N sol'n of HCl in diethyl ether (2.294 ml, 46.00 mmol). The reaction mixture was allowed to stir overnight at rt before being conc. in vacuo to afford the product as its hydrochloride salt. This material was dissolved in DMSO and purified via reverse phase HPLC to afford fractions that were conc. in vacuo, suspended in methanol (˜5 mL) and 1N HCl in diethyl ether (˜2 mL). This mixture was conc. in vacuo to afford (S,Z)-5-(2-(3-aminopyrrolidin-1-yl)-4-chlorobenzylidene)thiazolidine-2,4-dione hydrochloride (0.710 g, 64.3%). ¹H NMR (300 MHz, DMSO-D6) δ ppm 12.51 (s, 1H) 8.36 (s, 2H) 7.85 (s, 1H) 7.35 (d, 1H) 6.96 (dd, 2H) 3.90-3.75 (m, 1H) 3.55-3.47 (m, 1H) 3.40 (dd, 1H) 3.31-3.21 (m, 1H) 3.18-3.10 (m, 1H) 2.29-2.18 (m, 1H) 2.10-1.96 (m, 1H); m/z 325.

The following examples were prepared by the procedure of Example 85, using the appropriate starting materials. The following parent compounds obtained after chromatography may be converted to their corresponding hydrochloride salt in a manner similar as described in example 85, or similar procedure.

Ex. Compound ¹H NMR m/z SM 86 (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-3- chlorobenzylidene) thiazolidine-2,4-dione hydrochloride  

12.69 (s, 1 H) 8.35 (brs, 2 H) 7.94 (s 1 H) 7.57 (d, 1 H) 7.41-7.35 (m, 2 H) 3.95-3.88 (m, 1 H) 3.53- 3.26 (m, 4 H) 2.37- 2.31 (m, 1 H) 2.08-2.01 (m, 1 H) 324 (S,Z)-tert-butyl 1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) pyrrolidin-3-ylcarbamate Method 34  

87 (Z)-5-(2-(piperazin-1- yl)-5- (trifluoromethyl) benzylidene) thiazolidine- 2,4-dione hydroehloride  

12.69 (s, 1 H) 9.32 (brs, 2 H) 7.79 (d, 1 H) 7.71 (d, 2 H) 7.37 (d, 1 H) 3.23 (s, 8 H) 358 (Z)-tert-butyl 4-(2-((2,4- dioxothiazolidin-5- ylidene)methyl)-4- (trifluoromethyl)phenyl) piperazine-1-carboxylate Method 35  

88 (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-5- (trifluoromethyl) benzylidene) thiazolidine- 2,4-dione hydrochloride  

12.53 (s, 1 H) 8.17 (brs, 2 H) 7.95 (s, 1 H) 7.65 (s, 1 H) 7.59 (d 1 H) 7.05 (d, 1 H) 3.90-3.81 (m, 1 H) 3.56 (q, 1 H) 3.47-3.35 (m, 2 H) 3.18 (dd, 1 H) 2.33-2.18 (m, 1 H) 2.09- 1.99 (m, 1 H) 358 (S,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4- (trifluoromethyl)phenyl) pyrrolidin-3-ylcarbamate Method 36  

89 (Z)-5-((2-(piperazin- 1-yl)pyridin-3- yl)methylene) thiazolidine-2,4-dione hydrochloride  

12.65 (s, 1 H) 8.39 (s, 1 H) 8.33 (d, 1 H) 7.83 (d, 1 H) 7.63 (s, 1 H) 7.15 (dd, 1 H) 3.41 (brs, 4 H) 3.17 (brs, 4 H) 291 (Z)-tert-butyl 4-(3-((2,4- dioxothiazolidin-5- ylidene)methyl)pyridin- 2-yl)piperazine-1- carboxylate Method 37  

90 (Z)-5-((2-(4-(3- aminopropanoyl) piperazin-1-yl)pyridin-3- yl )methylene) thiazolidine-2,4-dione trifluoroacetate  

12.60 (s, 1 H) 8.32 (d, 1 H) 7.82 (s, 1 H) 7.68 (s, 1 H) 7.63 (brs, 2 H) 7.12 (dd, 1 H) 3.57 (d, 4 H) 3.17 (d, 4 H) 3.00 (q, 2 H) 2.70 (t, 2 H) 362 (Z)-tert-butyl 3-(4-(3- ((2,4-dioxothiazolidin-5- ylidene)methyl)pyridin- 2-yl)piperazin-1-yl)-3- oxopropylcarbamate Method 38  

91 (Z)-5-(2-(4- (piperidine-4- carbonyl)piperazin-1- yl)benzylidene) thiazolidine-2,4-dione  

9.00 (brs, 1 H) 8.67 (brs, 1 H) 8.18 (s, 1 H) 7.57- 7.52 (m, 1 H) 7.30-7.16 (m, 1 H) 3.71 (s, 1 H) 3.63 (s, 1 H) 3.34 (s, 3 H) 3.31-3.22 (m, 2 H) 3.13 (s, 3 H) 3.01-2.87 (m, 5 H) 1.85-1.77 (m, 4 H) 401 (Z)-tert-butyl 4-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperazine-1- carbonyl)piperidine-1- carboxylate Method 39  

92 (Z)-5-(2-(4- (piperidine-3- carbonyl)piperazin-1- yl)-5- (trifluoromethyl) benzylidene) thiazolidine- 2,4-dione hydrochloride  

12.68 (s, 1 H) 8.87 (s, 1 H) 7.78-7.69 (m, 3 H) 7.32 (d, 1 H) 3.68-3.64 (m, 8 H) 3.38 (tt, 1 H) 3.13-2.98 (m, 4 H) 1.75- 1.56 (m, 4 H) 469 (Z)-tert-butyl 3-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4- (trifluoromethyl)phenyl) piperazine-1- carbonyl)piperidine-1- carboxylate Method 40  

93 (Z)-5-(2-(4-(3- aminopropanoyl) piperazin-1-yl)-5- (trifluoromethyl) benzylidene) thiazolidine- 2,4-dione hydrochloride  

12.67 (s 1 H) 7.93 (brs, 2 H) 7.78-7.68 (m, 3 H) 7.32 (d, 1 H) 3.69-3.59 (m, 8 H) 3.00-2.74 (m, 4 H) 429 (Z)-tert-butyl 3-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4- (trifluoromethyl)phenyl) piperazin-1-yl)-3- oxopropylcarbamate Method 41  

94 (Z)-5-(2-(4-(3- aminopropanoyl) piperazin-1-yl)-3- methoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.53 (brs, 1 H) 8.21 (s, 1 H), 7.58 (brs, 3 H), 7.24 (t, 1 H) 7.11 (d, 1 H) 7.00 (d, 1 H) 3.75 (s, 3 H) 3.35 (brs, 4 H) 3.09- 2.85 (m, 4 H) 2.65 (brs, 2 H) 391 (Z)-tert-butyl 3-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6- methoxyphenyl) piperazin-1-yl)-3- oxopropylcarbamate Method 42  

95 (Z)-5-(2-(4-(3- aminopropanoyl) piperazin-1-yl)-3- chlorobenzylidene) thiazolidine-2,4-dione hydrochloride  

12.69 (brs, 1 H) 8.12 (s, 1 H) 7.67 (brs, 2 H) 7.55 (dd, 1 H) 7.49-7.40 (m, 1 H) 7.35 (t, 1 H) 4.17 (brs, 1 H) 3.75 (brs, 1 H) 3.35 (brs, 3 H) 3.13- 2.84 (m, 5 H) 2.74 (brs, 2 H) 395 (Z)-tert-butyl 3-(4-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperazin-1-yl)-3- oxopropylcarbamate Method 43  

96 (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-3- methoxybenzylidene) thiazolidine-2.4- dione hydrochloride  

12.43 (s, 1 H) 7.94 (s, 1 H) 7.86 (brs, 1 H) 7.16 (t, 1 H) 7.05 (d, 1 H) 6.91 (d, 1 H) 3.71 (s, 3 H) 3.30 (dd, 1 H) 3.17-3.08 (m, 1 H) 3.08-2.91 (m, 2 H) 2.23-2.06 (m, 2 H), 1.80-1.75 (m, 1 H) 320 (S,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6- methoxyphenyl) pyrrolidin-3-ylcarbamate Method 44  

97 (Z)-5-(3-methoxy-2- (piperazin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.45 (brs, 1 H) 8.59 (brs, 1 H) 7.99 (s, 1 H) 7.15 (t, 1 H) 7.02 (d, 1 H) 6.89 (d, 1 H) 3.67 (s, 3 H) 3.17-2.99 (m, 8 H) 320 (Z)-tert-butyl 4-(2-((2,4- dioxothiazolidin-5- ylidene)methyl)-6- methoxyphenyl) piperazine-1-carboxylate Method 45  

98 (Z)-5-(3-chloro-2- (piperazin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.72 (brs, 1 H) 9.19 (brs, 1 H) 8.01 (s, 1 H) 7.57 (dd, 1 H) 7.50-7.40 (m, 1 H) 7.36 (t, 1 H) 3.60 (brs, 2 H), 3.51- 3.29 (m, 1 H) 3.29-3.12 (m, 2 H) 3.06 (brs, 3 H) 324 (Z)-tert-butyl 4-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperazine-1-carboxylate Method 46  

99 (Z)-5-(2-(3- (aminomethyl)pyrrolidin- 1-yl)-4,5- dimethoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.40 (s, 1 H) 9.25 (s, 1 H) 7.88 (s, 1 H) 6.97 (s, 1 H) 6.73 (s, 1 H) 3.85 (s, 3 H) 3.81 (m, 1 H) 3.74 (s, 3 H) 3.40-3.31 (m, 2 H) 3.25-3.13 (m, 2 H) 2.59 (t, 3 H) 2.31 (dd, 1 H) 2.08 (d, 1 H) 364 (Z)-tert-butyl (1-(2-((2,4- dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3- yl)methylcarbamate Method 47  

100 (R,Z)-5-(2-(3- aminopyrrolidin-1- yl)-4,5- dimethoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.39 (s, 1 H) 8.06 (s, 2 H) 7.89 (s, 1 H) 6.96 (s, 1 H) 6.65 (s, 1 H) 3.84 (s, 4 H) 3.73 (s, 3 H) 3.35 (d, 2 H) 3.20 (s, 1 H) 3.07 (d, 1 H) 2.28 (s, 1 H) 1.97 (s, 1 H) 350 (R,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3-ylcarbamate Method 48  

101 (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-4,5- dimethoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.39 (s, 1 H) 8.08 (s, 2 H) 7.89 (s, 1 H) 6.96 (s, 1 H) 6.65 (s, 1 H) 3.84 (s, 4 H) 3.73 (s, 3 H) 3.45- 3.30 (m, 2 H) 3.24-3.14 (m, 1 H) 3.12-3.00 (m, 1 H) 2.28 (s, 1 H) 1.98 (s, 1H) 350 (S,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3- ylcarbamate Method 49  

102 (Z)-5-(2-(4- (piperidine-3- carbonyl)-1,4- diazepan-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.49 (s, 1 H) 8.47 (s, 1 H) 7.82 (d, 1 H) 7.39- 7.30 (m, 2 H) 7.16 (dd, 1 H) 7.06 (td, 1 H) 3.63- 3.54 (m, 4 H) 3.29-3.06 (m, 9 H) 1.88-1.48 (m, 6H) 415 (Z)-tert-butyl 3-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl)- 1,4-diazepane-1- carbonyl)piperidine-1- carboxylate Method 50  

103 (Z)-5-(2-(4-(5- aminopentanoyl)-1,4- diazepan-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.57 (s, 1 H) 7.90 (d, 1 H) 7.76 (s, 2 H) 7.47- 7.36 (m, 2 H) 7.22 (t, 1 H) 7.12 (d, 1 H) 3.63 (d, 4 H) 3.22 (s, 1 H) 3.17 (s, 1 H) 3.05 (s, 2 H) 2.80 (s, 2 H) 2.41 (s, 1 H) 2.34 (s, 1 H) 1.91 (s, 1 H) 1.85 (s, 1 H) 1.57 (d, 4 H) 403 (Z)-tert-butyl 5-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl)- 1,4-diazepan-1-yl)-5- oxopentylcarbamate Method 51  

104 (Z)-5-(2-(4-(4- aminobutanoyl)-1,4- diazepan-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.49 (s, 1 H) 7.83 (d, 1 H) 7.65 (s, 2 H) 7.39- 7.30 (m, 2 H) 7.20-7.12 (m, 1 H) 7.09-7.01 (m, 1 H) 3.61-3.50 (m, 4 H) 3.18-3.07 (m, 2 H) 2.98 (d, 2 H) 2.77 (ddd, 2 H) 2.37 (t, 2 H) 1.85-1.66 (m, 4 H). 389 (Z)-tert-butyl 4-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl)- 1,4-diazepan-1-yl)-4- oxobutylcarbamate Method 52  

105 (Z)-5-(2-(4-(3- aminopropanoyl)-1,4- diazepan-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.50 (s, 1 H) 7.83 (s, 1 H) 7.63 (s, 2 H) 7.39- 7.31 (m, 2 H) 7.16 (dd, 1 H) 7.10-7.01 (m, 1 H) 3.64-3.52 (m, 4 H) 3.20- 3.09 (m, 2 H) 2.97 (dt, 4 H) 2.65 (dt, 2 H) 1.87- 1.81 (m, 2 H) 375 (Z)-tert-butyl 3-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl)- 1,4-diazepan-1-yl)-3- oxopropylcarbamate Method 53  

106 (Z)-5-(2-(1,4- diazepan-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.57 (s, 1 H) 9.31 (s, 1 H) 7.91 (s, 1 H) 7.46- 7.40 (m, 2 H) 7.26 (d, 1 H) 7.18-7.09 (m, 1 H) 3.36 (d, 2 H) 3.27 (d, 4 H) 3.19 (dd, 2 H) 2.10- 2.01 (m, 2 H) 304 (Z)-tert-butyl 4-(2-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl)- 1,4-diazepane-1- carboxylate Method 54  

107 (Z)-5-(2-(4-(4- aminobutanoyl) piperazin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.60 (s, 1 H) 7.95 (s, 4 H) 7.53-7.44 (m, 2 H) 7.26-7.17 (m, 2 H) 3.66- 3.57 (m, 5 H) 2.89 (m, 4 H) 2.85-2.76 (m, 3 H) 1.85-1.76 (m, 2 H) 375 (Z)-tert-butyl 4-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperazin-1-yl)-4- oxobutylcarbamate Method 55  

108 (Z)-5-(2-(4-(5- aminopentanoyl) piperazin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.66 (s, 1 H) 8.10 (s, 2 H) 7.99 (s, 1 H) 7.52 (s, 1 H) 7.51-7.47 (m, 1 H) 7.25 (t, 2 H) 3.67 (s, 4 H) 2.92-2.81 (m, 6 H) 2.44 (t, 2 H) 1.62 (s, 4 H) 389 (Z)-tert-butyl 5-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperazin-1-yl)-5- oxopentylcarbamate Method 56  

109 (Z)-5-(2-(4-(4- (piperazin-1- ylmethyl)benzoyl) piperazin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.57 (brs, 1 H) 9.70 (brs, 1 H) 7.94 (s, 1 H) 7.75 (d, 2 H) 7.52 (m, 2 H) 7.49-7.41 (m, 2 H) 7.21 (t, 2 H) 4.45 (brs, 2 H) 3.81 (brs, 2 H) 3.54- 3.35 (m, 8 H) 3.27 (brs, 2 H) 2.89 (brs, 4 H) 492 (Z)-tert-butyl 4-(4-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperazine-1- carbonyl)benzyl)piperazine- 1-carboxylate Method 57  

110 (Z)-5-(2-(4-(3- (aminomethyl) benzoyl)piperazin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.56 (s, 1 H) 8.18 (brs, 2 H) 7.95 (s, 1 H) 7.64- 7.38 (m, 6 H) 7.36-7.11 (m, 2 H) 4.19-4.02 (m, 2 H) 3.82 (brs, 2 H) 3.52 (brs, 2 H) 2.92 (brs, 4 H) 423 (Z)-tert-butyl 3-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperazine-1- carbonyl)benzylcarbamate Method 58  

111 (Z)-5-(2-(3-(2- aminoethylamino) pyrrolidin-1-yl)-4,5- dimethoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.38 (s, 1 H) 9.99 (s, 1 H) 8.44 (s, 2 H) 7.86 (s, 1 H) 6.96 (s, 1 H) 6.76 (s, 1 H) 3.93 (s, 1 H) 3.84 (s, 3 H) 3.73 (s, 3 H) 3.45- 3.33 (m, 2 H) 3.28-3.16 (m, 6 H) 2.32 (s, 1 H) 2.15 (s, 1 H) 393 (Z)-tert-butyl 2-(1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3- ylamino)ethylcarbamate Method 59  

112 (Z)-5-(2-(4-(2- aminoethyl)piperazin- 1-yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.70 (s, 1 H) 11.43 (s, 1 H) 8.38 (s, 3 H) 7.86 (s, 1 H) 7.49 (m, 2 H) 7.25 (m, 2 H) 3.67 (m, 2 H) 3.42- 3.30 (m, 8 H) 333 (Z)-tert-butyl 2-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperazin-1- yl)ethylcarbamate Method 60  

113 (Z)-5-(4,5- dimethoxy-2-(3-(2- (methylamino) ethylamino)pyrrolidin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.39 (s, 1 H) 9.93 (brs, 1 H) 9.42 (brs, 1 H) 7.87 (s, 1 H) 6.98 (s, 1 H) 6.77 (s, 1 H) 4.00-3.91 (m, 1 H) 3.85 (s, 3 H) 3.74 (s, 3 H) 3.44-3.16 (m, 8 H) 2.62 (s, 3H) 2.40-2.31 (m, 1 H) 2.20-2.14 (m, 1 H) 407 (Z)-tert-butyl 2-(1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3- ylamino)ethyl)methyl) carbamate Method 61  

114 (Z)-5-(2-(4-(2- (methylamino)ethyl) piperazin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.62 (s, 1 H) 11.28 (s, 1 H) 9.28 (s, 1 H) 7.87 (s, 1 H) 7.50 (t, 1 H) 7.26 (t, 1 H) 3.71 (m, 4 H) 3.57 (m, 2 H) 3.45 (m, 2 H) 3.26 (m, 4 H) 2.62 (s, 3 H) 347 (Z)-tert-butyl 2-(4-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperazin-1- yl)ethyl)methyl)carbamate Method 62  

115 (5Z)-5-(2-piperazin- 1-ylbenzylidene)-1,3- thiazolidine-2,4- dione hydrochloride  

12.61 (s, 1 H) 9.21 (s, 1 H) 7.86 (s, 1 H) 7.49 (t, 2 H) 7.24 (t, 2 H), 3.25 (s, 4 H) 3.12 (s, 4 H) 290 tert-butyl-4-{2-[(Z)-(2,4- dioxo-1,3-thiazolidin-5- ylidene)methyl]phenyl} piperazine-1-carboxylate Example 58  

116 5-(2-(4-(piperidine-3- carbonyl)piperazin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.60 (s, 1 H) 9.10 (s, 1 H) 8.90 (s, 1 H) 7.94 (s, 1 H) 7.51-7.43 (m, 1 H) 7.21 (t, 2 H) 5.14 (m, 4 H) 3.67 (m, 3 H) 3.17 (m, 2 H) 2.91-2.88 (m, 4 H) 1.86 (s, 1 H) 1.81-1.71 (m, 2 H) 1.57 (d, 1 H) 401 tert-butyl 3-(4-(2-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperazine-1- carbonyl)piperidine-1- carboxylate Method 63  

117 (5Z)-5-{2-[4- (azetidin-3- ylcarbonyl)piperazin- 1-yl]benzylidene}- 1,3-thiazolidine-2,4- dione hydrochloride  

12.59 (s, 1 H) 9.15 (s, 1 H) 8.86 (s, 1 H) 7.93 (s, 1 H) 7.47 (ddd, 2 H) 7.25- 7.16 (m, 1 H) 4.15-4.05 (m, 4 H) 4.01-3.91 (m, 1 H) 3.71 (m, 2 H) 3.42 (s, 2 H) 2.89 (d, 4 H) 373 tert-butyl 3-(4-(2-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperazine-1- carbonyl)azetidine-1- carboxylate Method 64  

118 (5Z)-5-[2-(3- aminopyrrolidin-1- yl)-4,5- dimethoxybenzylidene]- 1,3-thiazolidine- 2,4-dione hydrochloride  

12.38 (s, 1 H) 8.34 (s, 2 H) 7.90 (s, 1 H) 6.96 (s, 1 H) 6.68 (s, 1 H) 3.89- 3.80 (m, 4 H) 3.73 (s, 3 H) 3.46-3.34 (m, 2 H) 3.23-3.13 (m, 1 H) 3.10 (dd, 1 H) 2.28 (dd, 1 H) 2.01 (d, 1 H) 351 tert-butyl 1-(2-((2,4- dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3-ylcarbamate Method 65  

119 (R,Z)-5-amino-N-(1- (2-((2,4- dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3- yl)pentanamide hydrochloride  

12.24 (s, 1 H) 8.07 (d, 1 H) 7.79 (s, 1 H) 7.60 (brs, 2 H) 6.88 (s, 1 H) 6.55 (s, 1 H) 4.22 (d, 1 H) 3.76 (s, 3 H) 3.65 (s, 3 H) 3.30-3.15 (m, 2 H) 3.15-2.98 (m, 1 H) 2.86 (dd, 1 H) 2.70 (d, 2 H) 2.17-1.92 (m, 3 H) 1.76 (brs, 1 H) 1.52-1.25 (m, 4 H) 449 (R,Z)-tert-butyl 5-(1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3-ylamino)-5- oxopentylcarbamate Method 66  

120 (S,Z)-5-amino-N-(1- (2-((2,4- dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3- yl)pentanamide hydrochloride  

12.24 (s, 1 H) 8.07 (d, 1 H) 7.79 (s, 1 H) 7.60 (brs, 2 H) 6.88 (s, 1 H) 6.55 (s, 1 H) 4.22 (d, 1 H) 3.76 (s, 3 H) 3.65 (s, 3 H) 3.30-3.15 (m, 2 H) 3.15-2.98 (m, 1 H) 2.86 (dd, 1 H) 2.70 (d, 2 H) 2.17-1.92 (m, 3 H) 1.76 (brs, 1 H) 1.52-1.25 (m, 4 H) 449 (S,Z)-tert-butyl 5-(1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3-ylamino)-5- oxopentylcarbamate Method 67  

121 (S,Z)-5-(2-(3-(3- aminopropylamino) pyrrolidin-1-yl)-4,5- dimethoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.50 (s, 1 H) 9.78 (s, 1 H) 7.92 (s, 1 H) 7.05 (s, 1 H) 6.85 (s, 1 H) 4.21- 4.11 (m, 1 H) 3.93 (s, 3 H) 3.83 (s, 3 H) 3.42- 3.25 (m, 8 H) 2.45 (m, 1 H) 2.20 (m, 1 H) 1.32 (m, 2 H) 407 (S,Z)-tert-butyl 3-(1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3- ylamino)propylcarbamate Method 68  

122A (R,Z)-5-(2-(3-(3- aminopropylamino) pyrrolidin-1-yl)-4,5- dimethoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.44 (s, 1 H) 9.78 (s, 1 H) 7.92 (s, 1 H) 7.05 (s, 1 H) 6.85 (s, 1 H) 4.21- 4.11 (m, 1 H) 3.93 (s, 3 H) 3.83 (s, 3 H) 3.42- 3.25 (m, 8 H) 2.45 (m, 1 H) 2.20 (m, 1 H) 1.32 (m, 2 H) 407 (R,Z)-tert-butyl 3-(1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-4,5- dimethoxyphenyl) pyrrolidin-3- ylamino)propylcarbamate Method 69  

122B (R,Z)-5-(2-(3- (aminomethyl) pyrrolidin-1-yl)-3- chlorobenzylidene) thiazolidine-2,4-dione  

(400 MHz, MeOD) ppm 8.08 (s, 1 H) 7.49 (dd, 2 H) 7.31 (t, 1 H) 3.56 (t, 1 H) 3.42-3.51 (m, 1 H) 3.38 (td, 1 H) 3.09-3.15 (m, 2 H) 2.71-2.78 (m, 1 H) 2.26-2.36 (m, 1 H) 1.86-1.96 (m, 1 H) 1.31 (dd, 1 H) 338 (R,Z)-tert-butyl (1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) pyrrolidin-3- yl)methylcarbamate Method 132  

122C (S,Z)-5-(2-(3- (aminomethyl) pyrrolidin-1-yl)-3- chlorobenzylidene) thiazolidine-2,4-dione  

(400 MHz, MeOD) ppm 8.07 (s, 1 H) 7.49 (dd, 2 H) 7.31 (t, 1 H) 3.56 (t, 1 H) 3.42-3.51 (m, 2 H) 3.38 (td, 1 H) 3.07-3.16 (m, 2 H) 2.67-2.79 (m, 1 H) 2.24-2.36 (m, 1 H) 1.90 (dd, 1 H) 338 (S,Z)-tert-butyl (1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)mcthyl)phenyl) pyrrolidin-3- yl)methylcarbamate Method 133  

122D (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-chlorobenzylidene) thiazolidine-2,4-dione hydrochloride  

12.70 (s, 1 H) 8.17 (brs, 3 H) 8.01 (s, 1 H) 7.52 (d, 1 H) 7.41-7.31 (m, 2 H) 3.40-3.10 (m, 4 H) 2.84- 2.81 (m, 1 H) 2.12-2.10 (m, 1 H) 1.80-1.58 (m, 3 H) 338 (R,Z)-tert-butyl 1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 134  

122E (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-methoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.60 (s, 1 H) 8.12 (brs, 4 H) 7.28-7.02 (m, 3 H) 3.82 (s, 3 H) 3.18-2.87 (m, 5 H) 2.10-2.07 (m, 1 H) 1.75-1.38 (m, 3 H) 334 (R,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6- methoxyphenyl)piperidin- 3-ylcarbamate Method 135  

122F (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-bromobenzylidene) thiazolidine-2,4-dione hydrochloride  

12.70 (s, 1 H) 8.15 (brs, 3 H) 8.01 (s, 1 H) 7.74- 7.69 (m, 1 H) 7.46 (d, 1 H) 7.26 (t, 1 H) 3.27-2.99 (m, 4 H) 2.82-2.80 (m, 1 H) 2.12-2.10 (m, 1 H) 1.76-1.40 (m, 3 H) 383 (R,Z)-tert-butyl 1-(2- bromo-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 136  

122G (R,Z)-5-(2-(3- aminopyrrolidin-1- yl)-3- chlorobrnzylidene) thiazolidine-2,4-dione hydrochloride  

12.67 (brs, 1 H) 8.30 (brs, 3 H) 7.93 (s, 1 H) 7.55 (d, 1 H) 7.41-7.31 (m, 2 H) 3.87 (brs, 1 H) 3.50-3.27 (m, 4 H) 2.40- 2.28 (m, 1 H) 2.08-1.95 (m, 1 H) 324 (R,Z)-tert-butyl 1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) pyrrolidin-3-ylcarbamate Method 137  

122H (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-3- bromobenzylidene) thiazolidine-2,4-dione hydrochloride  

12.68 (brs, 1 H) 8.31 (brs, 3 H) 7.90 (s, 1 H) 7.75 (d, 1 H) 7.46 (d, 1 H) 7.29 (t, 1 H) 3.75 (brs, 1 H) 3.55 (t, 1 H) 3.38- 3.25 (m, 3 H) 2.40-2.32 (m, 1 H) 2.12-2.02 (m, 1 H) 370 (S,Z)-tert-butyl 1-(2- bromo-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) pyrrolidin-3-ylcarbamate Method 138  

122I (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-ethoxybenzylidene) thiazolidine-2,4-dione hydrochloride  

12.58 (s, 1 H) 8.13-8.09 (m, 4 H) 7.26-7.24 (m, 1 H) 7.16-7.14 (m, 1 H) 7.03-7.01 (m, 1 H) 4.07 (q, 2 H) 3.78-3.67 (m, 2 H) 3.22-3.07 (m, 3 H) 2.10-2.07 (m, 1 H) 1.76- 1.55 (m, 3 H) 1.38 (t, 3 H) 348 (R,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6- ethoxyphenyl)piperidin- 3-ylcarbamate Method 139  

122J (R,Z)-tert-butyl 1-(2- ((2,4- dioxothiazolidin-5- ylidene)methyl)-6- isobutoxyphenyl) piperidin-3-ylcarbamate hydrochloride  

12.60 (s, 1 H) 8.18 (brs, 3 H) 8.15 (s, 1 H) 7.26 (t, 1 H) 7.15 (d, 1 H) 7.02 (d, 1 H) 3.84-3.74 (m, 2 H) 3.20-3.02 (m, 4 H) 2.74 (d, 1 H) 2.14-2.07 (m, 2 H) 1.73-1.21 (m, 3 H) 1.03 (d, 6 H) 376 (R,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6- isobutoxyphenyl) piperidin-3-ylcarbamate Method 140  

122K (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-(cyclohexylmethoxy) benzylidene) thiazolidine-2,4-dione hydrochloride  

12.60 (s, 1 H) 8.14 (brs, 4 H) 7.25 (t, 1 H) 7.14 (d, 1 H) 7.00 (d, 1 H) 3.82 (brs, 2 H) 3.29-3.00 (m, 4 H) 2.75-2.72 (m, 1 H) 2.12-2.10 (m, 1 H) 1.86- 1.50 (m, 8 H) 1.35-1.03 (m, 6 H) 416 (R,Z)-tert-butyl 1-(2- (cyclohexylmethoxy)-6- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 141  

122L (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-(cyclohexyloxy) benzylidene)thiazolidine- 2,4-dione hydrochloride  

402 (R,Z)-tert-butyl 1-(2- (cyclohexyloxy)-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 142  

122M (±)-(Z)-5-(2-3-amino- 4-hydroxypiperidin- 1-yl)-3- chlorobenzylidene) thiazolidine-2,4-dione hydrochloride  

12.68 (s, 1 H) 8.01 (brs, 4 H) 7.53 (d, 1 H) 7.44- 7.42 (m, 1 H) 7.35 (t, 1 H) 5.72-5.70 (m, 1 H) 3.32-3.22 (m, 2 H) 3.20- 3.11 (m, 3 H) 1.96-1.94 (m, 1 H) 1.40-1.37 (m, 1 H) 354 (±)-tert-butyl-1-(2- chloro-6-((Z)-(2,4- dioxothiazolidin-5- ylidene)methyl)phenyl)- 4-hydroxypiperidin-3- ylcarbamate Method 143  

122N (Z)-5-(3-chloro-2- (1,4-diazepan-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.73 (brs, 1 H), 9.18 (brs, 2 H), 8.04 (s, 1 H), 7.62 (d, 1 H), 7.41 (dt, 2 H), 3.65 (brs, 1 H), 3.34- 3.30 (m, 5 H), 3.04 (brs, 2 H), 2.14 (brs, 2 H) 338 (Z)-tert-butyl 4-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl)- 1,4-diazepane-1- carboxylate Method 144  

122O (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-isopropoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.61 (brs, 1 H) 8.33 (brs, 3 H) 8.15 (brs, 1 H) 7.34-7.09 (m, 2 H) 6.99 (d, 1 H) 4.67 (ddd 1 H) 3.28 (brs, 1 H) 3.07 (brs, 3 H) 2.70 (brs, 1 H) 2.12 (brs, 1 H) 1.73 (brs, 1 H) 1.57 (brs, 1 H) 1.51- 1.21 (m, 7 H) 362 (R,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6- isopropoxyphenyl) piperidin-3-ylcarbamate Method 145  

122P (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-3- isopropoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.58 (brs, 1 H) 8.52 (brs, 3 H) 8.08 (s, 1 H) 7.39-7.10 (m, 2 H) 6.99 (d, 1 H) 4.70 (d, 1 H) 3.78 (brs, 1 H) 3.52-3.33 (m, 1 H) 3.33-3.03 (m, 3 H) 2.28 (brs, 1 H) 2.00 (d, 1 H) 1.32 (d, 6 H) 348 (S,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6- isopropoxyphenyl) pyrrolidin-3-ylcarbamate Method 146  

122Q (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-3- ethoxybenzylidene) thiazolidine-2,4-dione hydrochloride  

12.58 (brs, 1 H) 8.48 (brs, 3 H) 8.09 (s, 1 H) 7.26 (t, 1 H) 7.16 (d, 1 H) 7.02 (d, 1 H) 4.09 (q, 2 H) 3.80 (d, 1 H) 3.47 (dd, 1 H) 3.28 (m, 1 H) 3.21 (dd, 1 H) 2.29 (d, 1 H) 2.01 (dd, 1 H) 1.40 (t, 3 H) 334 (S,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6- ethoxyphenyl)pyrrolidin- 3-ylcarbamate Method 147  

122R (R,Z)-5-(2-(3-(4- (aminomethyl) benzylamino)piperidin-1- yl)-3- chlorobenzylidene) thiazolidine-2,4-dione hydrochloride  

9.62 (brs, 1 H) 8.46 (brs, 3 H) 7.66-7.49 (m, 5 H) 7.47-7.34 (m, 2 H) 4.26- 4.12 (m, 2 H) 4.09-3.94 (m, 2 H) 3.42-3.25 (m, 4 H) 2.83 (brs, 1 H) 2.36 (s, 1 H) 1.85 (m, 1 H) 1.62 (d, 2 H) 457 (R,Z)-tert-butyl 4-((1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3- ylamino)methyl) benzylcarbamate Example 167  

122S (R,Z)-5-(3-chloro-2- (3-(2- (methylamino) ethylamino)piperidin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.68 (brs, 1 H) 9.68 (brs, 2H) 9.21 (brs, 2 H) 8.05 (s, 1 H) 7.56 (d, 1 H) 7.50-7.42 (m, 1 H) 7.37 (t, 1 H) 3.75-3.65 (m, 5 H) 3.63-3.57 (m, 2 H) 3.16 (d, 1 H) 2.82 (brs, 1 H) 2.60 (brs, 3 H) 2.37-2.28 (m, 1 H) 1.80 (d, 1 H) 1.65 (m, 1H) 1.54 (brs, 1 H) 395 (R,Z)-tert-butyl 2-(1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3- ylamino)ethyl)methyl) carbamate Example 168  

122T (Z)-5-(2-((3S,4S)-3- amino-4- hydroxypyrrolidin-1- yl)-3- chlorobenzylidene) thiazolidine-2,4-dione hydrochloride  

12.68 (brs, 1 H) 8.50 (brs, 3 H) 8.10-7.81 (m, 1 H) 7.58 (dd, 1 H) 7.51- 7.27 (m, 2 H) 4.56-4.28 (m, 1 H) 3.84 (brs, 1 H) 3.66-3.40 (m, 4 H) 3.39- 3.23 (m, 1 H) 339 tert-butyl (3S,4S)-1-(2- chloro-6-((Z)-(2,4- dioxothiazolidin-5- ylidene)methyl)phenyl)- 4-hydroxypyrrolidin-3- ylcarbamate Method 151  

122U (Z)-5-(3-chloro-2-(4- methyl-3- (methylamino) piperidin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.69 (brs, 1 H) 8.63 (brs, 2 H) 8.02 (s, 1 H) 7.57 (d, 1 H) 7.47-7.31 (m, 2 H) 3.58 (m, 1 H) 3.07 (m, 1 H) 2.67 (brs, 1 H) 2.58 (brs, 3 H) 2.40 (m, 1 H) 1.87 (m, 1 H) 1.68 (m, 1 H) 1.29 (m, 1 H) 1.07 (d, 3 H) 0.87 (d, 1 H) 365 (Z)-tert-butyl 1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl)- 4-methylpiperidin-3- yl)methyl)carbamate Method 152  

122V (Z)-5-(2-(3-amino-4- methylpiperidin-1- yl)-3- chlorobenzylidene) thiazolidine-2,4-dione hydrochloride  

351 (Z)-tert-butyl 1-(2- chloro-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl)- 4-methylpiperidin-3- ylcarbamate Method 153  

122W (R,Z)-5-(2-(3- aminopiperidin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.58 (s, 1 H), 8.44 (brs, 3 H) 7.89 (s, 1 H) 7.54- 7.34 (m, 2 H) 7.27-7.05 (m, 2 H) 3.26 (d, 2 H) 2.94 (d, 1 H), 2.87-2.61 (m, 2 H) 2.05 (brs, 1 H) 1.85 (brs, 1 H) 1.75-1.47 (m, 2 H) 304 (R,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 154   122X (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)benzylidene) thiazolidine-2,4-dione hydrochloride  

12.49 (s, 1 H) 8.50 (brs, 3 H) 7.91 (s, 1 H) 7.44- 7.22 (m, 2 H) 7.07-6.79 (m, 2 H) 3.83 (d, 1 H) 3.51-3.29 (m, 2 H) 3.28- 3.17 (m, 2 H) 2.27 (dd, 1 H), 2.11-1.94 (m, 1 H) 290 (S,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)phenyl) pyrrolidin-3-ylcarbamate Method 155  

122Y (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-(2,2,2- trifluoroethoxy) benzylidene) thiazolidine- 2,4-dione hydrochloride  

12.57 (brs, 1 H) 8.03 (brs, 1 H) 7.90 (brs, 3 H) 7.36-7.13 (m, 2 H) 7.07 (d, 1 H) 4.85-4.55 (m, 2 H) 2.98 (brs, 4 H) 2.74 (brs, 1 H) 2.02 (d, 1 H) 1.69 (d, 1 H) 1.54 (d, 1 H) 1.30 (brs, 1 H) 402 (R,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6-(2,2,2- trifluoroethoxy)phenyl) piperidin-3-ylcarbamate Method 156  

122Z (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-3-(2,2,2- trifluoroethoxy) benzylidene)thiazolidine- 2,4-dione hydrochloride  

12.52 (brs, 1 H) 8.21- 7.91 (m, 4 H) 7.34-7.14 (m, 2 H) 7.07 (d, 1 H) 4.88-4.72 (m, 2 H) 3.74 (d, 1 H) 3.40 (dd, 1 H) 3.22 (td, 1 H) 3.18-3.10 (m, 1 H) 3.06 (dd, 1 H) 2.30-2.10 (m, 1 H) 1.90 (dd, 1 H) 388 (S,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6-(2,2,2- trifluoroethoxy)phenyl) pyrrolidin-3-ylcarbamate Method 157  

122AA (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-(2- methoxyethoxy) benzylidene)thiazolidine- 2,4-dione hydrochloride  

12.67 (brs, 1 H) 8.30 (brs, 3 H) 8.20 (brs, 1 H) 7.33 (brs, 1 H) 7.25 (brs, 1 H) 7.10 (d, 1 H) 4.68 (brs, 3 H) 4.22 (t, 2 H) 3.91-3.65 (m, 2 H) 3.42 (s, 3 H) 3.29 (brs, 1 H) 2.80 (brs, 1 H) 2.18 (brs, 1 H) 1.81 (brs, 1 H) 1.68 (brs, 1 H) 1.48 (brs, 1 H) 378 (R,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6-(2- methoxyethoxy)phenyl) piperidin-3-ylcarbamate Method 158  

122AB (S,Z)-5-(2-(3- aminopyrrolidin-1- yl)-3-(2- methoxyethoxy) benzylidene)thiazolidine- 2,4-dione hydrochloride  

12.58 (brs, 1 H) 8.42 (brs, 3 H) 8.10 (s, 1 H) 7.28 (t, 1 H) 7.23-7.11 (m, 1 H) 7.04 (d, 1 H) 4.27-3.97 (m, 2 H) 3.85 (d, 1 H) 3.78-3.63 (m, 2 H) 3.49 (dd, 1 H) 3.34 (s, 3 H) 3.27 (td, 1 H) 3.22- 3.06 (m, 2 H) 2.30 (dd, 1 H) 2.02 (dd, 1 H) 364 (S,Z)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ylidene)methyl)-6-(2- methoxyethoxy)phenyl) pyrrolidin-3-ylcarbamate Method 159  

122AC (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-(cyclopentyloxy) benzylidene)thiazolidine- 2,4-dione hydrochloride  

12.37 (brs, 1 H) 8.00 (brs, 3H) 7.93 (s, 1 H) 7.02 (d, 1 H) 6.91 (d, 1 H) 6.77 (d, 1 H) 3.02- 2.91 (m, 1 H) 2.82 (d, 3 H) 2.48 (brs, 1 H) 1.89- 1.40 (m, 12 H) 1.16 (brs, 1 H) 388 (R,Z)-ten-butyl 1-(2- (cyclopentyloxy)-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 160  

122AD (R,Z)-5-(2-(3- aminopiperidin-1-yl)- 3-cyclobutoxybenzylidene) thiazolidine-2,4- dione hydrochloride  

12.60 (brs, 1 H) 8.14 (brs, 4 H) 7.23 (d, 1 H) 7.00 (dd, 2 H), 4.86-4.67 (m, 1 H) 3.25 (m, 1 H) 3.05 (m, 2 H) 2.74 (brs, 1 H) 2.49-2.35 (m, 2 H) 2.21-1.95 (m, 3 H) 1.93- 1.76 (m, 2 H) 1.67 (td, 3 H) 1.42 (brs, 1 H) 374 (R,Z)-tert-butyl 1-(2- cyclobutoxy-6-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 161  

122AE (R,Z)-4-(3- aminopipcridin-1-yl)- 3-((2,4- dioxothiazolidin-5- ylidene)methyl) benzamide hydrochloride  

347 (R,Z)-tert-butyl 1-(4- carbamoyl-2-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 162  

122AF (S,Z)-4-(3- aminopiperidin-1-yl)- 3-((2,4- dioxothiazolidin-5- ylidene)methyl) benzamide hydrochloride  

347 (S,Z)-tert-butyl 1-(4- carbamoyl-2-((2,4- dioxothiazolidin-5- ylidene)methyl)phenyl) piperidin-3-ylcarbamate Method 163  

122AG (R,Z)-4-(3- aminopiperidin-1-yl)- 3-((2,4- dioxothiazolidin-5- ylidene)methyl) benzoic acid hydrochloride  

348 (R,Z)-4-(3-(tert- butoxycarbonylamino) piperidin-1-yl)-3-((2,4- dioxothiazolidin-5- ylidene)methyl)benzoic acid Method 164  

122AH (S,Z)-4-(3- aminopyrrolidin-1- yl)-3-((2,4- dioxothiazolidin-5- ylidene)methyl) benzoic acid  

333 (S,Z)-4-(3-(tert- butoxycarbonylamino) pyrrolidin-1-yl)-3-((2,4- dioxothiazolidin-5- ylidene)methyl) benzoic acid Method 165  

122AI (R,Z)-5-((2-(3- aminopiperidin-1- yl)biphenyl-3- yl)methylene) thiazolidine-2,4- dione  

12.64 (brs, 1 H) 8.09 (brs, 3 H) 7.92 (s, 1 H) 7.56-7.36 (m, 4 H) 7.36- 7.16 (m, 4 H) 3.06 (brs, 1 H) 3.00 (brs, 1 H) 2.72 (d, 1 H) 2.35 (brs, 2 H) 2.05 (brs, 1 H) 1.58 (brs, 2 H) 1.32-1.15 (m, 1 H) 380 (R,Z)-tert-butyl 1-(3- ((2,4-dioxothiazolidin-5- ylidene)methyl)biphenyl- 2-yl)piperidin-3- ylcarbamate Method 166  

Example 123

(S,Z)-5-(2-(3-(3-aminopropylamino)pyrrolidin-1-yl)-5-(trifluoromethyl)benzylidene)thiazolidine-2,4-dione hydrochloride

A 25 mL round bottom flask was charged with a magnetic stir bar, (S,Z)-5-(2-(3-(3-(1,3-dioxoisoindolin-2-yl)propylamino)pyrrolidin-1-yl)-5-(trifluoromethyl)benzylidene)thiazolidine-2,4-dione (Method 70) (0.271 g, 0.50 mmol), EtOH (2.488 ml), and hydrazine (0.023 ml, 0.75 mmol). The reaction was stirred at rt for 30 min and then filtered through a bed of Celite. The filtrate was conc. in vacuo and purified via reverse phase HPLC (MeCN/water) to afford fractions that were conc. in vacuo, suspended in methanol (˜5 mL) and 1N HCl in diethyl ether (˜2 mL) and conc. in vacuo to afford (S,Z)-5-(2-(3-(3-aminopropylamino)pyrrolidin-1-yl)-5-(trifluoromethyl)benzylidene)thiazolidine-2,4-dione hydrochlride (0.071 g, 29.3%). ¹H NMR (300 MHz, DMSO-D6) δ ppm 12.52 (s, 1H) 9.49 (s, 1H) 8.08 (brs, 2H) 7.93 (s, 1H) 7.67 (s, 1H) 7.61 (d, 1H) 7.08 (d, 1H) 3.88-3.81 (m, 1H) 3.53-3.33 (m, 4H) 3.08-3.05 (m, 2H) 2.92-2.89 (m, 2H) 2.33-2.19 (m, 2H) 2.04-1.96 (m, 2H); m/z 415.

The following examples were prepared by the procedure of Example 123, using the appropriate starting materials. The following parent compounds obtained after chromatography may be converted to their corresponding hydrochloride salt in a manner similar as described in example 123.

Ex. Compound ¹H NMR m/z SM 124

12.59 (brs, 1 H) 9.30 (brs, 1 H) 8.09 (s, 1 H) 8.07 (brs, 1 H) 7.32 (t, 1 H) 7.21 (d, 1 H) 7.08 (d, 1 H) 3.87 (s, 3 H) 3.46-3.44 (m, 1 H) 3.39-3.25 (m, 2 H) 3.17-2.99 (m, 4 H) 2.96-2.91 (m 2 H) 2.19-2.10 (m, 2 H) 2.08-1.99 (m, 2 H) 377

125

12.72 (brs, 1 H) 9.50 (brs, 1 H) 8.10 (brs, 2 H) 7.96 (s, 1 H) 7.60 (d, 1 H) 7.46-7.39 (m, 2 H) 3.95-3.91 (m, 1 H) 3.51-3.20 (m, 4 H) 2.99- 2.92 (m, 2 H) 2.44-2.38 (m, 2 H) 2.21-2.11 (m, 2 H) 2.08-1.99 (m, 2 H) 382

126

12.40 (s, 1 H) 9.78 (s, 1 H) 7.92 (s, 1 H) 7.05 (s, 1 H) 6.85 (s, 1 H) 4.21-4.11 (m, 1 H) 3.93 (s, 3 H) 3.83 (s, 3 H) 3.42-3.25 (m, 8 H) 2.45 (m, 1 H) 2.20 (m, 1 H) 1.32 (m, 2 H) 407

127

12.64 (s, 1 H) 11.50 (s, 1 H) 8.27 (s, 1 H) 7.85 (s, 1 H) 7.49 (t, 2 H) 7.24 (t, 2 H) 3.55 (d, 2 H) 3.32- 3.16 (m, 8 H) 2.93 (d, 2 H) 2.18-2.07 (m, 2 H) 347

128

12.34 (s, 1 H) 8.46 (d, 1 H) 7.86 (s, 3 H) 6.95 (s, 1 H) 6.63 (s, 1 H) 4.36-4.26 (m, 1 H) 3.84 (s, 3 H) 3.72 (s, 3 H), 3.32-3.21 (m, 3 H) 3.02-2.93 (m, 3 H) 2.18 (dd, 2 H) 1.86 (dd, 1 H) 421

129A

12.59 (s, 1 H) 7.94 (s, 1 H) 7.82 (s, 2 H) 7.51- 7.44 (m, 2 H) 7.26-7.17 (m, 2 H) 3.66 (m, 2 H) 3.59 (m, 2 H) 3.08-2.97 (m, 2 H) 2.96-2.86 (m, 4 H) 2.74 (m, 2 H) 361

129B

12.69 (brs, 1 H) 9.31 (brs, 1 H) 8.05 (s, 3 H) 7.56 (d, 1 H) 7.49-7.41 (m, 1 H) 7.37 (t, 1 H) 3.76-3.65 (m, 3 H) 3.16 (brs, 1 H) 3.04 (brs, 2 H) 2.94-2.75 (m, 3 H) 2.33 (brs, 1 H) 2.04-1.92 (m, 2 H) 1.78 (d, 1 H) 1.65 (m, 1 H), 1.50 (m, 1 H) 395

129C

12.64 (brs, 1 H) 7.94 (brs, 4 H) 7.11 (m, 2 H) 6.93 (s, 1 H) 4.13 (m, 2 H) 3.77 (s, 3 H) 2.97 (m, 2 H) 2.06 (m, 2 H) 309

Example 130

(5Z)-5-[2-(4-acetylpiperazin-1-yl)benzylidene]-1,3-thiazolidine-2,4-dione: To a mixture of (57)-5-(2-piperazin-1-ylbenzylidene)-1,3-thiazolidine-2,4-dione (Example 115) and acetyl chloride (36.1 mg, 0.46 mmol) was added triethylamine (93 mg, 0.92 mmol) at room temperature. The reaction mixture was stirred at room temperature overnight and was then treated with sat'd aqueous NaHCO₃ (˜25 mL). This mixture was allowed to stir at room temperature for 10 min, and was then extracted with DCM (3×25 mL). The combined organic extract was dried over anhydrous Na₂SO₄, filtered through a bed of Celite, and the filtrate was conc. in vacuo to afford the product which was purified via reverse phase HPLC (acetonitrile:water: 0.1% TFA=5% to 70%) to afford the title compound as a pale yellow solid (40.0 mg, 35.4%). ¹H NMR (300 MHz, DMSO-D6) δ ppm 12.58 (brs, 1H) 7.94 (s, 1H) 7.47 (d, 2H) 7.30-7.07 (m, 2H) 3.60 (brs, 4H) 2.90 (brs, 2H) 2.85 (brs, 2H) 2.04 (s, 3H); m/z 331.

The following examples were prepared by the procedure of Example 130, using the appropriate starting materials.

Ex. Compound ¹H NMR m/z SM 131A

12.29 (brs, 1 H) 8.13 (d, 1 H) 7.85 (s, 1 H) 6.94 (s, 1 H) 6.61 (s, 1 H) 4.27 (d, 1 H) 3.83 (s, 3 H) 3.72 (s, 3 H) 3.43-3.14 (m, 4 H) 2.94 (dd, 1 H) 2.30-2.05 (m, 1 H) 1.82 (s, 3 H) 392

131B

9.86 (s, 1 H) 7.68 (s, 1 H) 7.56 (s, 1 H) 7.49-7.47 (d, 1 H) 6.96-6.94 (d, 1 H) 3.23 (m, 1 H) 3.00 (m, 4 H) 2.51 (s, 6 H) 2.15 (m, 1 H) 2.01 (s, 3 H) 1.55 (m, 1 H) 375

Example 132

(Z)-5-(2-(3-(2-hydroxyethylamino)pyrrolidin-1-yl)-4,5-dimethoxybenzylidene)thiazolidine-2,4-dione

A mixture of (Z)-5-(2-(3-(2-(tert-butyldimethylsilyloxy)ethylamino)pyrrolidin-1-yl)-4,5-dimethoxybenzylidene)thiazolidine-2,4-dione (Method 77) (120 mg, 0.24 mmol) in 5 mL of 1.25 M HCl in methanol was stirred at room temperature for 30 min. The mixture was then conc. in vacuo to afford the product which was purified via reverse phase HPLC (acetonitrile:water: 0.1% NH₄OAc=5% to 55%) to yield the title compound as a yellow solid (45.0 mg, 48.4%). ¹H NMR (300 MHz, DMSO-D6) δ ppm 7.40 (s, 1H) 7.07 (s, 1H) 6.57 (s, 1H) 3.78 (s, 3H) 3.70 (s, 3H) 3.45 (m, 2H) 3.27-3.15 (m, 4H) 2.92 (m, 1H) 2.59 (m, 2H) 2.08 (m, 1H) 1.66 (m, 1H); m/z 394.

The following examples were prepared by the procedure of Example 132 using the appropriate starting materials.

133

8.81 (brs, 2 H) 7.96 (s, 1 H) 7.48 (d, 1 H) 7.40-7.32 (m, 1 H) 7.31-7.25 (m, 1 H) 3.63-3.55 (m, 2 H) 3.46-3.38 (m, 2 H) 3.08 (brs, 2 H) 2.96 (d, 2 H) 2.75 (brs, 1 H) 2.20 (m, 1H) 1.72 (brs, 1 H) 1.56 (brs, 1 H) 1.44 (brs, 1 H) 382

134

12.69 (brs, 1 H) 8.84 (brs, 1 H) 8.04 (s, 1 H) 7.56 (d, 1 H) 7.49-7.39 (m, 1 H) 7.36 (t, 1 H) 3.69 (dd, 2 H) 3.17 (brs, 2 H) 3.00 (brs, 2 H) 2.83 (brs, 1 H) 2.26 (brs, 1 H) 1.93-1.72 (m, 4 H) 1.66 (brs, 1 H) 1.50 (brs, 1 H)

Example 135

(S,Z)—N-(1-(2-chloro-6-((2,4-dioxothiazolidin-5-ylidene)methyl)phenyl)pyrrolidin-3-yl)-1-methyl-1H-imidazole-2-carboxamide

To a 50 mL vial charged with a magnetic stir bar was added (S,Z)-5-(2-(3-aminopyrrolidin-1-yl)-3-chlorobenzylidene)thiazolidine-2,4-dione (75 mg, 0.23 mmol) (Example 86), 1-methyl-1H-imidazole-2-carboxylic acid (87 mg, 0.69 mmol), HATU (220 mg, 0.58 mmol) and dichloromethane (5 mL). Hunig's base (0.202 mL, 1.16 mmol) was then added and the mixture was stirred at rt for 4 h. The reaction was then diluted with dichloromethane and washed with water. The mixture was separated with a phase separator tube and the organic phase was evaporated to dryness. The residue was purified by reverse phase chromatography to afford the title compound as a yellow solid (21 mg, 21%). ¹H NMR (400 MHz, DMSO-d₆) δ ppm 7.89 (s, 1H) 7.51 (dd, 1H) 7.41 (dd, 1H) 7.18-7.27 (m, 2H) 7.01 (s, 1H) 4.67-4.77 (m, 1H) 4.00 (s, 3H) 3.63-3.75 (m, 2H) 3.47-3.54 (m, 1H) 3.36-3.47 (m, 1H) 2.39-2.51 (m, 1H) 2.14 (dd, 1H); m/z 432.

The following examples were prepared by the procedure of 135 using the appropriate starting materials.

136

7.89 (s, 1 H) 7.52 (dd, 1 H) 7.41 (dd, 1 H) 7.25 (t, 1 H) 4.58-4.67 (m, 1 H) 3.93 (s, 2 H) 3.65 (dd, 1 H) 3.36- 3.46 (m, 5 H) 3.13 (dd, 1 H) 2.38 (dd, 1 H) 2.01-2.13 (m, 1 H) 396

137

7.93 (s, 1 H) 7.61 (d, 1 H) 7.52 (dd, 1 H) 7.42 (dd, 1 H) 7.25 (t, 1 H) 6.73 (d, 1 H) 4.73-4.83 (m, 1 H) 3.92-3.99 (m, 3 H) 3.71 (dd, 1 H) 3.38-3.50 (m, 2 H) 3.23 (dd, 1 H) 2.38-2.50 (m, 1 H) 2.09-2.21 (m, 1 H) 432

138

7.93 (s, 1 H) 7.52 (dd, 1 H) 7.41 (dd, 1 H) 7.25 (t, 1 H) 4.53 (ddd, 1 H) 3.83-3.92 (m, 2 H) 3.65-3.70 (m, 1 H) 3.42-3.49 (m, 1 H) 3.35-3.42 (m, 1 H) 3.07 (dd, 1 H) 2.37 (dt, 1 H) 1.98- 2.10 (m, 1 H) 424

139

8.44 (br. S., 2 H) 8.02 (s, 1 H) 7.83 (d, 1 H) 7.50 (dd, 1 H) 7.34-7.46 (m, 3 H) 7.25 (t, 1 H) 4.51 (t, 1 H) 3.60-3.69 (m, 3 H) 3.37-3.48 (m, 2 H) 3.08 (dd, 1 H) 2.31-2.43 (m, 1 H) 2.03 (dd, 1 H) 443

140

8.47 (br. S., 1 H) 7.98 (s, 1 H) 7.53 (dd, 1 H) 7.41 (dd, 3 H) 7.25 (t, 1 H) 4.53 (t, 1 H) 3.64-3.71 (m, 3 H) 3.39-3.48 (m, 2 H) 3.07 (dd, 1 H) 2.32-2.44 (m, 1 H) 2.03 (dd, 1 H) 443

141

8.17 (s, 1 H) 8.07 (s, 1 H) 7.94 (s, 1 H) 7.47 (ddd, 2 H) 7.27 (t, 1 H) 4.67-4.78 (m, 1 H) 3.89-3.97 (m, 3 H) 3.73 (dd, 1 H) 3.44 (t, 2 H) 3.19 (dd, 1 H) 2.42 (dd, 1 H) 2.14 (dd, 1 H) 432

142

7.91 (s, 1 H) 7.56 (dd, 1 H) 7.43 (dd, 1 H) 7.27 (t, 1 H) 4.62 (t, 1 H) 3.67- 3.78 (m, 2 H) 3.41-3.48 (m, 2 H) 3.19-3.22 (m, 2 H) 3.10-3.17 (m, 5 H) 2.92-2.98 (m, 2 H) 2.81-2.86 (m, 1 H) 2.37-2.48 (m, 1 H) 2.02-2.13 (m, 1 H) 483

143

7.18 (s, 1 H) 6.70 (dd, 1 H) 6.62 (dd, 1 H) 6.45 (t, 1 H) 3.71 (s, 1 H) 2.84 (dd, 1 H) 2.56- 2.66 (m, 2 H) 2.29-2.36 (m, 2 H) 2.26 (dd, 1 H) 1.64 (t, 2 H) 1.57 (d, 1 H) 1.25-1.37 (m, 2 H) 1.20 (d, 1 H) 473

144

8.01 (s, 1 H) 7.50 (d, 1 H) 7.44 (d, 1 H) 7.26 (t, 1 H) 4.47-4.58 (m, 1 H) 3.62 (t, 1 H) 3.42 (t, 2 H) 3.02-3.15 (m, 4 H) 2.92 (s, 3 H) 2.67 (d, 2 H) 2.56 (t, 2 H) 2.36 (dd, 1 H) 2.02 (dd, 1 H) 451

145

7.07 (s, 1 H) 6.69-6.74 (m, 1 H) 6.59 (dd, 1 H) 6.43 (t, 1 H) 3.76 (br. S., 1 H) 2.85-2.94 (m, 2 H) 2.84 (d, 1 H) 2.36 (br. S., 1 H) 2.29 (dd, 2 H) 1.52-1.64 (m, 7 H) 1.18-1.28 (m, 1 H) 409

146

7.95 (s, 1 H) 7.52 (dd, 1 H) 7.42 (dd, 1 H) 7.26 (t, 1 H) 4.50 (ddd, 1 H) 3.68-3.78 (m, 1 H) 3.64 (dd, 1 H) 3.44- 3.50 (m, 1 H) 3.40 (t, 1 H) 3.22 (q, 1 H) 3.09 (dd, 1 H) 2.39 (dt, 1 H) 2.03 (d, 1 H) 391

147

7.89 (s, 1 H) 7.54 (dd, 1 H) 7.39 (dd, 1 H) 7.24 (t, 1 H) 4.49- 4.60 (m, 1 H) 3.86- 3.98 (m, 1 H) 3.65 (dd, 1 H) 3.36-3.48 (m, 2 H) 3.17- 3.25 (m, 1 H) 3.07-3.16 (m, 1 H) 2.31-2.43 (m, 1 H) 1.96-2.09 (m, 4 H) 423

Example 148

(R,Z)-5-(3-chloro-2-(3-(dipropylamino)piperidin-1-yl)benzylidene)thiazolidine-2,4-dione

A mixture of (R,Z)-5-(2-(3-aminopiperidin-1-yl)-3-chlorobenzylidene)thiazolidine-2,4-dione (122D) (100 mg, 0.27 mmol) and propionaldehyde (20.17 mg, 0.35 mmol) in CH₂Cl₂ (15 mL) was stirred at 50° C. for 20 min. before sodium triacetoxyborohydride (170 mg, 0.80 mmol) was added. The mixture was then stirred at 50° C. for 4 h. before sat'd aqueous K₂CO₃ (˜50 mL) was added to the mixture. This solution was poured into a separatory funnel and extracted with CHCl₃/isopropanol (5/1) (2×50 mL). The combined organic extract was dried over anhydrous Na₂SO₄, filtered, and conc. in vacuo affording the product. It was purified with Gilson (0.1% TFA in water:0.1% TFA in CAN=30% to 80%; UV absorption at 322) to yield the title compound as a yellow solid (94 mg, 77%). ¹H NMR (400 MHz, DMSO-d₆) δ ppm 7.96 (s, 1H) 7.48 (d, 1H) 7.41-7.34 (m, 1H) 7.29 (t, 1H) 3.67-3.58 (m, 2H) 3.44-3.37 (m, 2H) 3.03-2.78 (m, 5H) 2.21 (brs, 1H), 1.79 (d, 1H) 1.61 (d, 6H) 0.84-0.79 (m, 6H). m/z 422.

He following examples were prepared by the procedure of 148, using the appropriate starting materials.

149

12.65 (brs, 1 H) 9.35 (brs, 1H) 8.05 (s, 1 H) 7.56 (d, 1 H) 7.44 (brs, 1 H) 7.37 (t, 1 H) 3.75-3.63 (m, 2 H) 3.53-3.45 (m, 2 H) 3.23 (m, 1 H) 2.85- 2.65 (m, 4 H) 2.30 (brs, 1 H) 1.85-1.76 (m, 1 H) 1.67 (brs, 1 H) 1.49 (brs, 1 H) 433

150

12.65 (s, 1H), 8.91 (s, br, 2H), 7.91 (s, 1H), 7.10 (m, 2H), 7.85 (s, 1H), 4.17 (m, 2H), 3.65 (m, 4H), 3.05 (m, 2H), 2.14 (m, 2H), 1.22 (m, 6H) 351

Example 151

(Z)-5-(5-amino-2-(3-(dimethylamino)pyrrolidin-1-yl)benzylidene)thiazolidine-2,4-dione

To a mixture of (Z)-5-(2-(3-(dimethylamino)pyrrolidin-1-yl)-5-nitrobenzylidene)thiazolidine-2,4-dione (100 mg, 0.28 mmol) (Method 149) and iron (154 mg, 2.76 mmol) chip in MeOH (10 mL) was added 5 drops of conc. HCl and 5 drops of water. The mixture was stirred at 80° C. for 1 hr. The mixture was loaded into silica gel, purified with ISCO (100% ethyl acetate to methanol/ethyl acetate=50%) to yield a red solid which was repurified with Gilson (acetonitrile:water:0.1% TFA=0% to 50% fro 7 min) to yield a yellow solid as (Z)-5-(5-amino-2-(3-(dimethylamino)pyrrolidin-1-yl)benzylidene)thiazolidine-2,4-dione (51.0 mg, 38.6%). ¹H NMR (400 MHz, DMSO-d6) d ppm 12.55 (brs, 1H), 11.32 (brs, 1H), 10.30 (brs, 2H), 7.80 (s, 1H), 7.42 (m, 2H), 7.19 (m, 1H), 3.95 (m, 1H), 3.39-3.27 (m, 4H), 2.78 (s, 6H), 2.30-2.10 (m, 2H); m/z 333.

The following intermediates were prepared by the procedure of Example 151 using the appropriate starting materials.

Ex Compound ¹H NMR m/z SM 152

12.45 (brs, 1 H) 7.87 (s, 1 H) 6.80 (s, 1H) 6.79 (s, 1 H) 3.78 (s, 3 H) 3.73 (s, 3 H) 280

Methods Section

Method 1 (S)-3-bromo-2-(3-(dimethylamino)pyrrolidin-1-yl)benzaldehyde

A 50 mL round bottom flask was charged with a magnetic stir bar, 3-bromo-2-fluorobenzaldehyde (0.555 g, 2.73 mmol), (S)—N,N-dimethylpyrrolidin-3-amine (0.312 g, 2.73 mmol), DMSO (5.47 ml), and potassium carbonate (0.378 g, 2.73 mmol). The mixture was heated to 85° C. overnight with stirring. The reaction was allowed to cool to ambient temperature, was diluted with water, and extracted into methylene chloride. The combined organic extract was dried with MgSO₄, filtered, and conc. in vacuo to provide the product which was purified via silica gel chromatography (40 g) using ethyl acetate/MeOH (10:1) as eluent to afford (S)-3-bromo-2-(3-(dimethylamino)pyrrolidin-1-yl)benzaldehyde (0.369 g, 45.4%).

The following intermediates were prepared by the procedure of Method 1, using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 2

287

3

249

4

249

5

254

6

253

7

279

8

279

9

280

10

266

11

249

12

263

13

253

14

236

15

207

16

219

17

233

18

233

19

267

20

279

21

293

22

293

23

359

24

264

25

262

26

291

79

326

80

359

81

359

82

292

83

322

84

321

85

326

86

365

87

351

88

351

89

305

90

351

91

339

92

339

93

339

94

335

95

385

96

325

97

371

98

349

99

377

100

417

101

403

102

354

103

10.49 (s, 1 H), 7.75 (d, 1 H), 7.63 (d, 1 H), 7.25 (t, 1 H), 3.37 (brs, 4 H), 1.88 (brs, 2 H), 1.53 (s, 9 H) 339

104

249

105

287

106

253

107

233

108

237

109

233

110

298

111

237

112

253

113

363

114

349

115

334

116

263

117

10.30 (s, 1 H) 7.76 (dd, 1 H) 7.65 (dd, 1 H) 7.29-7.24 (m, 1 H) 5.05 (brs, 1 H) 4.41 (brs, 1 H) 4.20 (brs, 1 H) 3.87 (dd, 1 H) 3.78 (dd, 1 H) 3.64-3.47 (m, 1 H) 3.36 (brs, 1 H) 3.21 (dd, 1 H) 1.50 (s, 9 H) 341

118

367

119

310

120

305

121

291

122

403

123

389

124

379

125

365

126

389

127

375

128

348

129

348

130

348

131

334

Method 27 5-methoxy-2-(2-(piperidin-1-yl)ethoxy)benzaldehyde

A mixture of 2-hydroxy-5-methoxybenzaldehyde (0.761 g, 5 mmol), 1-(2-chloroethyl)piperidine hydrochloride (0.921 g, 5.00 mmol), K₂CO₃ (2.07 g, 14.99 mmol), and sodium iodide (0.075 g, 0.50 mmol) in acetonitrile (40 mL) was stirred at 100° C. overnight. The reaction was allowed to cool to ambient temperature and sat'd aqueous K₂CO₃ was added to the reaction mixture. The mixture was poured into a reparatory funnel and extracted with EtOAc. The organic phase was dried over anhydrous MgSO₄, filtered through a bed of Celite, and conc. in vacuo to yield the product which was purified via silica gel chromatography (80 g) using EtOAc/hexanes (4:1) as eluent to yield the title compound as a brown oil (0.551 g, 42%); m/z 264.

The following intermediates were prepared by the procedure of Method 27, using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 28 5-methoxy-2-(2- 266 2-hydroxy-5- morpholinoethoxy) methoxy- benzaldehyde benzaldehyde

and 4-(2- chloroethyl)morpholine hydrochloride

29 2-(2- 252 2-hydroxy-5- (diethylamino) methoxy- ethoxy)-5- benzaldehyde methoxybenzaldehyde

and 2-chloro-N,N- diethylethanamine

30A 2-(2- 222 Salicylaldehyde (diethylamino)ethoxy) benzaldehyde

and 2-chloro-N,N- diethylethanamine

30B 2-(3-(1,3- 10.18 (s, 1 H) 7.69 (m, 4 340 2-hydroxy-5- dioxoisoindolin-2- H) 7.11 (m, 2 H) 6.97 (d, methoxybenzaldehyde yl)propoxy)-5- 1 H) 4.05 (m, 2 H) 3.83 methoxybenzaldehyde (m, 2 H) 3.67 (s, 3 H) 2.12 (m, 2 H)

and 2-(3- bromopropyl) isoindoline-1,3-dione

Method 31 4,5-dimethoxy-2-(pyridin-3-yl)benzaldehyde

A mixture of 2-bromo-4,5-dimethoxybenzaldehyde (0.368 g, 1.5 mmol), pyridin-3-ylboronic acid (0.246 g, 2.0 mmol), Pd(PPh₃)₄ (0.173 g, 0.150 mmol), and cesium carbonate (0.977 g, 3 mmol) were suspended in dioxane (4 mL) and water (1 mL). The mixture was heated to 140° C. in a microwave for 1 h. The reaction vessel was allowed to cool to ambient temperature, diluted with ethyl acetate (˜25 mL), filtered through a bed of Celite, and conc. in vacuo to afford the aldehyde which was purified via SiO₂ chromatography (40 g) using ethyl acetate/hexanes (5:1) as eluent to afford the title compound as a white solid (0.340 g, 93%); m/z 244.

The following intermediates were prepared by the procedure of Method 31, using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 32A 4,5-dimethoxy-2- 244 2-bromo-4,5- (pyridin-4- dimethoxy- yl)benzaldehyde benzaldehyde

and pyridin-4-ylboronic acid

32B N-(2- 9.95 (s, 1 H), 8.05 (d, 1 346 N-(2- (dimethylamino)ethyl)- H), 7.96 (d, 2 H), 7.72- (dimethylamino)ethyl)- 2′-formyl-N- 7.83 (m, 1 H), 7.57- N-methyl-4-(4,4,5,5- methylbiphenyl-4- 7.72 (m, 3 H), 7.53 (d, 1 tetramethyl-1,3,2- sulfonamide H), 3.24 (t, 2 H), 2.79- dioxaborolan-2- 2.91 (m, 3 H), 2.65 (t, 2 yl)benzenesulfonamide H), 2.37 (s, 6 H)

And 2-bromobenzaldehyde

Method 33 (S,Z)-tert-butyl 1-(5-chloro-2-((2,4-dioxothiazolidin-5-ylidene)methyl)phenyl)pyrrolidin-3-ylcarbamate

A 100 mL round bottom flask was charged with a magnetic stir bar, (S)-tert-butyl 1-(5-chloro-2-formylphenyl)pyrrolidin-3-ylcarbamate (Method 78) (1.100 g, 3.39 mmol), thiazolidine-2,4-dione (0.397 g, 3.39 mmol), and ethanol (11.29 ml). Piperidine (0.034 mL, 0.34 mmol) was added and the reaction was heated to reflux for 2 h. Once the reaction was judged to be complete by LCMS, it was allowed to cool to ambient temperature and conc. in vacuo to afford the title compound (1.310 g, 91%) which was used in the next step without further purification.; m/z 425.

The following intermediates were prepared by the procedure of Method 33, using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 34 (S,Z)-tert-butyl 1-(2- 425 tent-butyl [(3S)-1-(2- chloro-6-((2,4- chloro-6- dioxothiazolidin-5- formylphenyl)pyrrolidin- ylidene)methyl)phenyl) 3-yl]carbamate pyrrolidin-3-ylcarbamate Method 79

35 (Z)-tert-butyl 4-(2-((2,4- 459 tert-butyl4-[2-formyl-4- dioxothiazolidin-5- (trifluoromethyl)phenyl] ylidene)methyl)-4- piperazine-1-carboxylate (trifluoromethyl)phenyl) Method 80 piperazine-1-carboxylate

36 (S,Z)-tert-butyl 1-(2- 459 tert-butyl {(3S)-1-[2- ((2,4-dioxothiazolidin-5- formyl-4- ylidene)methyl)-4- (trifluoromethyl)phenyl] (trifluoromethyl)phenyl) pyrrolidin-3-yl} carbamate pyrrolidin-3-ylcarbamate Method 81

37 (Z)-tert-butyl 4-(3-((2,4- 391 tert-butyl 4-(3- dioxothiazolidin-5- formylpyridin-2- ylidene)methyl)pyridin- yl)piperazine-1- 2-yl)piperazine-1- carboxylate carboxylate Method 82

44 (S,Z)-tert-butyl 1-(2- 421 tert-butyl [(3S)-1-(2- ((2,4-dioxothiazolidin-5- formyl-6- ylidene)methyl)-6- methoxyphenyl) methoxyphenyl) pyrrolidin-3-yl]carbamate pyrrolidin-3-ylcarbamate Method 83

45 (Z)-tert-butyl 4-(2-((2,4- 421 tert-butyl 4-(2-formyl-6- dioxothiazolidin-5- methoxyphenyl)piperazine- ylidene)methyl)-6- 1-caboxylate methoxyphenyl)piperazine- Method 84 1-carboxylate

46 (Z)-tert-butyl 4-(2- 425 tert-butyl 4-(2-chloro-6- chloro-6-((2,4- formylphenyl)piperazine- dioxothiazolidin-5- 1-carboxylate ylidene)methyl)phenyl) Method 85 piperazine-1-carboxylate

47 (Z)-tert-butyl (1-(2-((2,4- 465 tert-butyl (1-(2-formyl- dioxothiazolidin-5- 4,5- ylidene)methyl)-4,5- dimethoxyphenyl) dimethoxyphenyl) pyrrolidin-3- pyrrolidin-3- yl)methylcarbamate yl)methylcarbamate Method 86

48 (R,Z)-tert-butyl 1-(2- 451 (R)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-4,5- ylidene)methyl)-4,5- dimethoxyphenyl) dimethoxyphenyl) pyrrolidin-3-ylcarbamate pyrrolidin-3-ylcarbamate Method 87

49 (S,Z)-tert-butyl 1-(2- 451 (S)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-4,5- ylidene)methyl)-4,5- dimethoxyphenyl) dimethoxyphenyl) pyrrolidin-3-ylcarbamate pyrrolidin-3-ylcarbamate Method 88

54 (Z)-tert-butyl 4-(2-((2,4- 404 tert-butyl 4-(2- dioxothiazolidin-5- formylphenyl)-1,4- ylidene)methyl)phenyl)- diazepane-1-carboxylate 1,4-diazepane-1- Method 89 carboxylate

65 tert-butyl 1-(2-((2,4- 451 tert-butyl 1-(2-formyl- dioxothiazolidin-5- 4,5- ylidene)methyl)-4,5- dimethoxyphenyl) dimethoxyphenyl) pyrrolidin-3-carbamate pyrrolidin-3-ylcarbamate Method 90

132 (R,Z)-tert-butyl (1-(2- 439 (R)-tert-butyl (1-(2- chloro-6-((2,4- chloro-6- dioxothiazolidin-5- formylphenyl)pyrrolidin- ylidene)methyl)phenyl) 3-yl)methylcarbamate pyrrolidin-3-yl) Method 91 methylcarbamate

133 (S,Z)-tert-butyl (1-(2- 439 (S)-tert-butyl (1-(2- chloro-6-((2,4- chloro-6- dioxothiazolidin-5- formylphenyl)pyrrolidin- ylidene)methyl)phenyl) 3-yl)methylcarbamate pyrrolidin-3-yl)methylcarbamate Method 92

134 (R,Z)-tert-butyl 1-(2- 438 (R)-tert-butyl 1-(2- chloro-6-((2,4- chloro-6- dioxothiazolidin-5- formylphenyl)piperidin- ylidene)methyl)phenyl) 3-ylcarbamate piperidin-3-ylcarbamate Method 93

135 (R,Z)-tert-butyl 1-(2- 434 (R)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-6- ylidene)methyl)-6- methoxyphenyl)piperidin- methoxyphenyl) 3-ylcarbamate pyrrolidin-3-ylcarbamate Method 94

136 (R,Z)-tert-butyl 1-(2- 484 (R)-tert-butyl bromo-6-((2,4- bromo-6- dioxothiazolidin-5- formylphenyl)piperidin- ylidene)methyl)phenyl) 3-ylcarbamate piperidin-3-ylcarbamate Method 95

137 (R,Z)-tert-butyl 1-(2- 424 (R)-tert-butyl 1-(2- chloro-6-((2,4- chloro-6- dioxothiazolidin-5- formylphenyl)pyrrolidin- ylidene)methyl)phenyl) 3-ylcarbamate pyrrolidin-3-ylcarbamate Method 96

138 (S,Z)-tert-butyl 1-(2- 470 (S)-tert-butyl 1-(2- bromo-6-((2,4- bromo-6- dioxothiazolidin-5- formylphenyl)pyrrolidin- ylidene)methyl)phenyl) 3-ylcarbamate pyrrolidin-3-ylcarbamate Method 97

139 (R,Z)-tert-butyl 1-(2- 448 (R)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ethoxy-6- ylidene)methyl)-6- formylphenyl)piperidin- ethoxyphenyl) 3-ylcarbamate piperidin-3-ylcarbamate Method 98

140 (R,Z)-tert-butyl 1-(2- 476 (R)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-6- ylidene)methyl)-6- isobutoxyphenyl)piperidin- isobutoxyphenyl) 3-ylcarbamate piperidin-3-ylcarbamate Method 99

141 (R,Z)-tert-butyl 1-(2- 516 (R)-tert-butyl 1-(2- (cyclohexylmethoxy)-6- (cyclohexylmethoxy)-6- ((2,4-dioxothiazolidin-5- formylphenyl)piperidin- ylidene)methyl)phenyl) 3-ylcarbamate piperidin-3-ylcarbamate Method 100

142 (R,Z)-tert-butyl 1-(2- 501 (R)-tert-butyl 1-(2- (cyclohexyloxy)-6-((2,4- (cyclohexyloxy)-6- dioxothiazolidin-5- formylphenyl)piperidin- ylidene)methyl)phenyl) 3-ylcarbamate piperidin-3-ylcarbamate Method 101

143 (±)-tert-butyl 1-(2- 454 (±)-tert-butyl-1-(2- chloro-6-((Z)-((2,4- chloro-6-formylphenyl)- dioxothiazolidin-5- 4-hydroxypiperidin-3- ylidene)methyl)phenyl)- ylcarbamate 4-hydroxypiperidin-3- Method 102 ylcarbamate

144 (Z)-tert-butyl 4-(2- 8.66 (brs, 438 tert-butyl 4-(2-chloro-6- chloro-6-((2,4- 1 H), 8.36 formylphenyl)-1,4- dioxothiazolidin-5- (brs, 1 H), diazepane-1-carboxylate ylidene)methyl)phenyl)- 7.47 (d, 1 Method 103 1,4-diazepane-1- H), 7.39 carboxylate (d, 1 H), 7.22 (t, 1 H), 3.61 (brs, 3 H), 3.52 (brs, 2 H), 3.27 (brs, 3 H), 1.91 (brs, 2 H), 1.52 (s, 9 H)

145 (R,Z)-tert-butyl 1-(2- 462 (R)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-6- ylidene)methyl)-6- isopropoxyphenyl) isopropoxyphenyl) piperidin-3-ylcarbamate piperidin-3-ylcarbamate Method 113

146 (S,Z)-tert-butyl 1-(2- 448 (S)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-6- ylidene)methyl)-6- isopropoxyphenyl) isopropoxyphenyl) pyrrolidin-3-ylcarbamate pyrrolidin-3-ylcarbamate Method 114

147 (S,Z)-tert-butyl 1-(2- 334 (S)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- ethoxy-6- ylidene)methyl)-6- formylphenyl)pyrrolidin- ethoxyphenyl) 3-tlcarbamate pyrrolidin-3-ylcarbamate Method 115

148 5-(2-(3-(1,3- 12.54 (s, 438 2-(3-(1,3- dioxoisoindolin-2- 1 H) 7.83- dioxoisoindolin-2- yl)propoxy)-5- 7.79 (m, 5 yl)propoxy)-5- methoxybenzylidene) H) 7.04 (s, methoxybenzaldehyde thiazolidine-2,4-dione 2 H) 6.86 Method 30B (s, 1 H) 4.08 (m, 2 H) 3.80- 3.75 (m, 5 H) 2.11 (m, 2 H)

149 5-(2-(3- 8.20 (s, 1 363 2-(3- (dimethylamino)pyrrolidin- H) 8.07 (d, (dimethylamino)pyrrolidin- 1-yl)-5- 1 H) 7.82 1-yl)-5- nitrobenzylidene) (s, 1 H) nitrobenzaldehyde thiazolidine-2,4-dione 6.96 (d, Method 116 1H) 3.58- 3.51 (m, 3H) 3.36 (m, 1 H) 3.04 (m, 1 H) 2.51 (s, 6 H), 2.40 (m, 1 H), 1.86 (m, 1 H)

150 (Z)-5-(4,5-dimethoxy-2- 8.32 (s, 1 309 4,5-dimethoxy-2- nitrobenzylidene) H) 8.25 nitrobenzaldehyde thiazolidene-2,4-dione (brs, 1 H) 7.79 (s, 1 H) 7.02 (s, 1 H) 4.03 (s, 6 H)

151 tert-butyl (3S,4S)-1-(2- 8.30 (s, 1 H) 439 tert-butyl (3S,4S)-1-(2- chloro-6-((Z)-(2,4- 7.49 (d, chloro-6-formylphenyl)- dioxothiazolidin-5- 1 H) 7.42 4-hydroxypyrrolidin-3- ylidene)methyl)phenyl)- (d, 1 H) ylcarbamate 4-hydroxypyrrolidin-3- 7.28-7.20 Method 117 ylcarbamate (m, 1 H) 5.01 (d, 1 H) 4.39 (brs, 1 H) 4.27 (brs, 1 H) 4.05 (s, 1 H) 3.83 (dd, 1 H) 3.69 (dd, 1 H) 3.27 (brs, 1 H) 3.19 (dd, 1 H), 1.50 (s, 9 H)

152 (Z)-tert-butyl 1-(2- 465 tert-butyl 1-(2-chloro-6- chloro-6-(2,4- formylphenyl)-4- dioxothiazolidin-5- methylpiperidin-3- ylidene)methyl)phenyl)- yl(methyl)carbamate 4-methylpiperidin-3- Method 118 yl(methyl)carbamate

153 (Z)-tert-butyl 1-(2- 451 tert-butyl 1-(2-chloro-6- chloro-6-((2,4- formylphenyl)-4- dioxothiazolidin-5- methylpiperidin-3- ylidene)methyl)phenyl)- ylcarbamate 4-methylpiperidin-3- Method 119 ylcarbamate

154 (R,Z)-tert-butyl 1-(2- 404 (R)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formylphenyl)piperidin- ylidene)methyl)phenyl)- 3-ylcarbamate piperidin-3-ylcarbamate Method 120

155 (S,Z)-tert-butyl 1-(2- 390 (S)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formylphenyl)pyrrolidin- ylidene)methyl)phenyl)- 3-ylcarbamate pyrrolidin-3-ylcarbamate Method 121

156 (R,Z)-tert-butyl 1-(2- 502 (R)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-6-(2,2,2- ylidene)methyl)-6-(2,2,2- trifluoroethoxy)phenyl) trifluoroethoxy)phenyl) piperidin-3-ylcarbamate piperidin-3-ylcarbamate Method 122

157 (S,Z)-tert-butyl 1-(2- 488 (S)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-6-(2,2,2- ylidene)methyl)-6-(2,2,2- trifluoroethoxy)phenyl) trifluoroethoxy)phenyl) pyrrolidin-3-ylcarbamate pyrrolidin-3-ylcarbamate Method 123

158 (R,Z)-tert-butyl 1-(2- 478 (R)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-6-(2- ylidene)methyl)-6-(2- methoxyethoxy)phenyl) methoxyethoxy)phenyl) piperidin-3-ylcarbamate piperidin-3-ylcarbamate Method 124

159 (S,Z)-tert-butyl 1-(2- 464 (S)-tert-butyl 1-(2- ((2,4-dioxothiazolidin-5- formyl-6-(2- ylidene)methyl)-6-(2- methoxyethoxy)phenyl) methoxyethoxy)phenyl) pyrrolidin-3-ylcarbamate pyrrolidin-3-ylcarbamate Method 125

160 (R,Z)-tert-butyl 1-(2- 488 (R)-tert-butyl 1-(2- (cyclopentyloxy)-6-((2,4- (cyclopentyloxy)-6- dioxothiazolidin-5- formylphenyl)piperidin- ylidene)methyl)phenyl) 3-ylcarbamate piperidin-3-ylcarbamate Method 126

161 (R,Z)-tert-butyl 1-(2- 474 (R)-tert-butyl 1-(2- cyclobutoxy)-6-((2,4- cyclobutoxy-6- dioxothiazolidin-5- formylphenyl) ylidene)methyl)phenyl) piperidin-3-ylcarbamate piperidin-3-ylcarbamate Method 127

162 (R,Z)-tert-butyl 1-(2- 447 (R)-tert-butyl 1-(4- carbamoyl-2-((2,4- carbamoyl-2- dioxothiazolidin-5- formylphenyl) ylidene)methyl)phenyl) piperidin-3-ylcarbamate piperidin-3-ylcarbamate Method 128

163 (S,Z)-tert-butyl 1-(4- 447 (S)-tert-butyl 1-(4- carbamoyl-2-((2,4- carbamoyl-2- dioxothiazolidin-5- formylphenyl) ylidene)methyl)phenyl) piperidin-3-ylcarbamate piperidin-3-ylcarbamate Method 129

164 (R,Z)-4-(3-(tert- 447 (R)-4-(3-(tert- butoxycarbonylamino) butoxycarbonylamino) piperidin-1-yl)-3-((2,4- piperidin-1-yl)-3- dioxothiazolidin-5- formylbenzoic acid ylidene)methyl)benzoic Method 130 acid

165 (S,Z)-4-(3-(tert- 433 (S)-4-(3-(tert- butoxycarbonylamino) butoxycarbonylamino) pyrrolidin-1-yl)-3-((2,4- pyrrolidin-1-yl)-3- dioxothiazolidin-5- formylbenzoic acid ylidene)methyl)benzoic Method 131 acid

166 (R,Z)-tert-butyl 1-(3- 480 (R)-tert-butyl 1-(3- ((2,4-dioxothiazolidin-5- formylbiphenyl-2- ylidene)methyl)biphenyl- yl)piperidin-3- 2-yl)piperidin-3- ylcarbamate ylcarbamate Method 201

Method 38 (Z)-tert-butyl 3-(4-(3-((2,4-dioxothiazolidin-5-ylidene)methyl)pyridin-2-yl)piperazin-1-yl)-3-oxopropylcarbamate

A 50 mL vial was charged with a magnetic spin bar, (Z)-5-((2-(piperazin-1-yl)pyridin-3-yl)methylene)thiazolidine-2,4-dione hydrochloride (Example 89) (0.125 g, 0.38 mmol), 3-(tert-butoxycarbonylamino)propanoic acid (0.109 g, 0.57 mmol), DMF (1.912 ml), and diisopropylethylamine (0.334 ml, 1.91 mmol). With stirring, HATU (0.291 g, 0.76 mmol) was added and the reaction was warmed to 50° C. for 3 h. The reaction was then diluted with water and extracted with ethyl acetate (3×50 mL). The combined organic extract was dried with MgSO₄, filtered through a bed of Celite, and conc. in vacuo to yield the product which was purified via silica gel chromatography (80 g) using ethyl acetate/hexanes (1:1) as eluent to provide the title compound as an off white solid. (0.080 g, 45.3%); m/z 462.

The following intermediates were prepared by the procedure of Method 38, using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 39 (Z)-tert-butyl 4-(4-(2- 502 (5Z)-5-(2-piperazin-1- ((2,4-dioxothiazolidin-5- ylbenzylidene)-1,3- ylidene)methyl)phenyl) thiazolidine-2,4-dione piperazine-1- carbonyl)piperidine-1- carboxylate

Example 115 and 1-(tert- butoxycarbonyl)piperidine- 4-carboxylic acid

40 (Z)-tert-butyl 3-(4-(2- 560 (Z)-5-(2-(piperazin-1-yl)- ((2,4-dioxothiazolidin-5- 5-(trifluoromethyl) ylidene)methyl)-4- benzylidene) (trifluoromethyl)phenyl) thiazolidine-2,4- piperazine-1- dione carbonyl)piperidine-1- carboxylate

Example 87 and 1-(tert- butoxycarbonyl)piperidine- 3-carboxylic acid

41 (Z)-tert-butyl 3-(4-(2- 530 (Z)-5-(2-(piperazin-1-yl)- ((2,4-dioxothiazolidin-5- 5-(trifluoromethyl) ylidene)methyl)-4- benzylidene) (trifluoromethyl)phenyl) thiazolidine-2,4- piperazin-1-yl)-3- dione oxopropylcarbamate

Example 87 and 3-[(tert- butoxycarbonyl)amino] propanoic acid

42 (Z)-tert-butyl 3-(4-(2- 492 (Z)-5-(3-methoxy-2- ((2,4-dioxothiazolidin-5- (piperazin-1-yl)- ylidene)methyl)-6- benzylidene) methoxyphenyl)piperazin- thiazolidine-2,4- 1-yl)-3- dione oxopropylcarbamate

Example 97 and 3-[(tert- butoxycarbonyl)amino] propanoic acid

43 (Z)-tert-butyl 3-(4-(2- 496 (Z)-5-(3-chloro-2- chloro-6-((2,4- (piperazin-1-yl)- dioxothiazolidin-5- benzylidene) ylidene)methyl)phenyl) thiazolidine-2,4- piperazin-1-yl)-3- dione oxopropylcarbamate

Example 98 and 3-[(tert- butoxycarbonyl)amino] propanoic acid

50 (Z)-tert-butyl 3-(4-(2- 516 (Z)-5-(2-(1,4-diazepan-1- ((2,4-dioxothiazolidin-5- yl)benzylidene) ylidene)methyl)phenyl)- thiazolidine-2,4-dione 1,4-diazepane-1- carbonyl)piperidine-1- carboxylate

Example 106 and 1-(tert- butoxycarbonyl)piperidine- 3-carboxylic acid

51 (Z)-tert-butyl 5-(4-(2- 504 (Z)-5-(2-(1,4-diazepan-1- ((2,4-dioxothiazolidin-5- yl)benzylidene) ylidene)methyl)phenyl)- thiazolidine-2,4-dione 1,4-diazepan-1-yl)-5- oxopentylcarbamate

Example 106 and 5-[(tert- butoxycarbonyl)amino] pentanoic acid

52 (Z)-tert-butyl 4-(4-(2- 490 (Z)-5-(2-(1,4-diazepan-1- ((2,4-dioxothiazolidin-5- yl)benzylidene) ylidene)methyl)phenyl)- thiazolidine-2,4-dione 1,4-diazepan-1-yl)-4- oxobutylcarbamate

Example 106 and 4-[(tert- butoxycarbonyl)amino] butanoic acid

53 (Z)-tert-butyl 3-(4-(2- 475 (Z)-5-(2-(1,4-diazepan-1- ((2,4-dioxothiazolidin-5- yl)benzylidene) ylidene)methyl)phenyl)- thiazolidine-2,4-dione 1,4-diazepan-1-yl)-3- oxopropylcarbamate

Example 106 and 3-[(tert- butoxycarbonyl)amino] propanoic acid

55 (Z)-tert-butyl 4-(4-(2- 476 (5Z)-5-(2-piperazin-1- ((2,4-dioxothiazolidin-5- yl)benzylidene)-1,3- ylidene)methyl)phenyl) thiazolidine-2,4-dione piperazin-1-yl)-4- oxobutylcarbamate

Example 115 and 4-[(tert- butoxycarbonyl)amino] butanoic acid

56 (Z)-tert-butyl 5-(4-(2- 490 (5Z)-5-(2-piperazin-1- ((2,4-dioxothiazolidin-5- ylbenzylidene)-1,3- ylidene)methyl)phenyl) thiazolidine-2,4-dione piperazin-1-yl)-5- oxopentylcarbamate

Example 115 and nd 5-[(tert- butoxycarbonyl)amino] pentanoic acid

57 (Z)-tert-butyl 4-(4-(4-(2- 593 (5Z)-5-(2-piperazin-1- ((2,4-dioxothiazolidin-5- ylbenzylidene)-1,3- ylidene)methyl)phenyl) thiazolidine-2,4-dione piperazine-1- carbonyl)benzyl) piperazine-1-carboxylate

Example 115 and 4-{[4-(tert- butoxycarbonyl)piperazin- 1-yl]methyl}benzoic acid

58 (Z)-tert-butyl 3-(4-(2- 524 (5Z)-5-(2-piperazin-1- ((2,4-dioxothiazolidin-5- yl)benzylidene)-1,3- ylidene)methyl)phenyl) thiazolidine-2,4-dione piperazine-1- carbonyl)benzylcarbamate

Example 115 and 3-{[(tert- butoxycarbonyl)amino) methyl}benzoic acid

63 tert-butyl 3-(4-(2- 501 (5Z)-5-(2-piperazin-1- ((2,4-dioxothiazolidin-5- ylbenzylidene)-1,3- ylidene)methyl)phenyl) thiazolidine-2,4-dione piperazine-1- carbonyl)piperidine-1- carboxylate

Example 115 And 1-(tert- butoxycarbonyl)piperidine- 3-carboxylic acid

64 tert-butyl 3-(4-(2- 473 (5Z)-5-(2-piperazin-1- ((2,4-dioxothiazolidin-5- ylbenzylidene)-1,3- ylidene)methyl)phenyl) thiazolidine-2,4-dione piperazine-1- carbonyl)azetidine-1- carboxylate

Example 115 And 1-(tert- butoxycarbonyl)azetidine- 3-carboxylic acid

66 (R,Z)-tert-butyl 5-(1-(2- 550 (R,Z)-5-(2-(3- ((2,4-dioxothiazolidin-5- aminopyrrolidin-1-yl)- ylidene)methyl)-4,5- 4,5- dimethoxyphenyl) dimethoxybenzylidene) pyrrolidin-3-ylamino)-5- thiazolidine-2,4-dione oxopentylcarbamate

Example 100 And 5-[(tert- butoxycarbonyl)amino] pentanoic acid

67 (S,Z)-tert-butyl 5-(1-(2- 550 (S,Z)-5-(2-(3- ((2,4-dioxothiazolidin-5- aminopyrrolidin-1-yl)- ylidene)methyl)-4,5- 4,5- dimethoxyphenyl) dimethoxybenzylidene) pyrrolidin-3-ylamino)-5- thiazolidine-2,4-dione oxopentylcarbamate

Example 101 And 5-[(tert- butoxycarbonyl)amino] pentanoic acid

75 (Z)-3-(1,3- 552 (5Z)-5-[2-(3- dioxoisoindolin-2-yl)-N- aminopyrrolidin-1-yl)- (1-(2-((2,4- 4,5- dioxothiazolidin-5- dimethoxybenzylidene]- ylidene)methyl)-4,5- 1,3-thiazolidine-2,4-dione dimethoxyphenyl) pyrrolidin-3-yl) propanamide

Example 118 and 3-(1,3-dioxo-1,3- dihydro-2H-isoindol-2- yl)propanoic acid

76 5Z)-(2-(4-(3-(1,3- (5Z)-5-(2-piperazin-1- dioxoisoindolin-2- ylbenzylidene)-1,3- yl)propanoyl)piperazin-1- thiazolidine-2,4-dione yl)benzylidene) thiazolidine-2,4-dione

Example 115 and 3-(1,3-dioxo-1,3- dihydro-2H-isoindol-2- yl)propanoic acid

Method 59 (Z)-tert-butyl 2-(1-(2-((2,4-dioxothiazolidin-5-ylidene)methyl)-4,5-dimethoxyphenyl)pyrrolidin-3-ylamino)ethylcarbamate

A mixture of (5Z)-5-{2-[3-(dimethylamino)pyrrolidin-1-yl]-4,5-dimethoxybenzylidene}-1,3-thiazolidine-2,4-dione (Example 52) (120 mg, 0.31 mmol) and tert-butyl 2-oxoethylcarbamate (198 mg, 1.24 mmol) in CH₂Cl₂ (20 mL) were heated to reflux for 15 min followed by the addition of sodium triacetoxyhydroborate (65.9 mg, 0.31 mmol). The reaction mixture was refluxed overnight before being allowed to cool to ambient temperature. Water (˜0.5 mL) was added and the mixture was allowed to stir for 15 min before being loaded onto a silica gel column which was eluted with ethyl acetate/hexane (10:1) to yield the title compound as an orange solid as (60.0 mg, 39.2%); m/z 493.

The following intermediates were prepared by the procedure of Method 59, using the appropriate starting materials.

Method Compound ¹H NMR m/z SM  60

433

 61

507

 62

448

 68

507

 69

507

 70

545

 71

508

 72

512

 73

507

 74

477

 77

509

167

557

168

496

169

496

170

510

171

525

Method 172 3-ethoxy-2-fluorobenzaldehyde

A 200 mL round bottom flask was charged with a magnetic stir bar, 3-ethoxy-2-fluorobenzonitrile (1.000 g, 6.05 mmol), and anhydrous toluene (12.92 ml). The sol'n was placed under argon and cooled to 0° C. with an ice bath. DIBAL-H (7.27 ml, 7.27 mmol) (1M in PhMe) was then added drop wise via syringe and the reaction was allowed to stir to rt overnight. To this mixture was added 10% HCl until the sol'n reached a pH of ˜2. The resulting mixture was then left to stir for 0.5 h. and was then poured into a separatory funnel and extracted with ethyl acetate (2×200 mL). The combined organic extract was dried with MgSO₄, filtered, and conc. in vacuo to yield the crude product which was purified via silica gel chromatography (80 g) using ethyl acetate/hexanes (1:4) as eluent to provide pure 3-ethoxy-2-fluorobenzaldehyde (0.810 g, 80%). m/z 196.

The following intermediates were prepared by the procedure of Method 172, using the appropriate starting materials.

173

197

174

237

175

223

176

183

177

223

178

10.32 (s, 1 H) 7.56- 7.35 (m, 2 H) 7.13- 6.96 (m, 1 H) 4.33- 4.22 (m, 2 H) 3.84- 3.77 (m, 2 H) 3.42 (s, 3 H) 199

179

195

180

10.31 (s, 1 H) 7.41- 7.36 (m, 2 H) 7.05- 7.03 (m, 1 H) 4.94- 4.85 (m, 1 H) 2.01- 1.83 (m, 8 H) 209

Method 181

A 100 mL round bottom flask was charged with a magnetic stir bar, 2-fluoro-3-hydroxybenzonitrile (0.500 g, 3.65 mmol), MeCN (13.89 ml), 1-bromo-2-methylpropane (0.699 ml, 7.29 mmol), and K₂CO₃ (1.008 g, 7.29 mmol). The mixture was then placed in an oil bath and heated to 60° C. with stirring overnight. This mixture was then cooled to rt, filtered through a bed of Celite, and conc. In vacuo. The crude material was purified via silica gel chromatography (40 g) using ethyl acetate/hexanes (1:4) as eluent to afford pure 2-fluoro-3-isobutoxybenzonitrile (0.610 g, 87%) as a colorless oil. M/z 194.

The following intermediates were prepared by the procedure of Method 181, using the appropriate starting materials.

182

234

183

220

184

179

185

196

186

192

187

Method 188

(±)-tert-butyl-4-hydroxypyrrolidin-3-ylcarbamate

A racemic mixture of trans-benzyl 3-(tert-butoxycarbonylamino)-4-hydroxypyrrolidine-1-carboxylate (3.36 g, 9.99 mmol) (Method 190) in MeOH (40 mL) was degassed, then 10 wt % Pd/C (3.19 g, 3.00 mmol) was added. The mixture was degassed, charged with H₂, and stirred overnight. The mixture was filtered through a bed of Celite, washed with methanol, the filtrate was dried over anhydrous Na₂SO₄, filtered and conc. in vacuo to yield a white solid as racemic tert-butyl-4-hydroxypyrrolidin-3-ylcarbamate (2.020 g, 100%). ¹H NMR (400 MHz, MeOD) δ □ppm 4.13 (dt, 1H), 3.89-3.75 (m, 1H), 3.35 (brs, 1H), 3.14 (dd, 1H), 2.93-2.73 (m, 2H), 1.46 (s, 10H); m/z 203.

The following intermediates were prepared by the procedure of Method 188, using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 189

216

Method 190

(±)-benzyl 3-(tert-butoxycarbonylamino)-4-hydroxypyrrolidine-1-carboxylate

To a mixture of racemic trans-benzyl 3-amino-4-hydroxypyrrolidine-1-carboxylate (2.67 g, 11.30 mmol) (Method 193) and di-tert-butyl dicarbonate (3.70 g, 16.95 mmol) in CH₂Cl₂ (50 mL) was added Et₃N (4.73 mL, 33.90 mmol) at 0° C. The mixture was then stirred at rt for 48 h. The reaction mixture was then conc. in vacuo giving a residue which was purified via silica gel chromatography (40% to 90% ethyl acetate/hexane) to yield a white solid as (±)-benzyl 3-(tert-butoxycarbonylamino)-4-hydroxypyrrolidine-1-carboxylate (3.36 g, 88%). ¹H NMR (400 MHz, Dichloromethane-d2) δ □ppm 7.48-7.32 (m, 5H), 5.21-5.00 (m, 2H), 4.78 (brs, 1H), 4.29-4.18 (m, 1H), 3.84 (dd, 1H), 3.74 (d, 1H), 3.41-3.12 (m, 2H), 1.49-1.33 (m, 9H); m/z 337

The following starting materials were prepared by the procedure of Method 190 using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 191

350

192

352

Method 193

(±)-benzyl 3-amino-4-hydroxypyrrolidine-1-carboxylate

A mixture of racemic trans-benzyl 3-azido-4-hydroxypyrrolidine-1-carboxylate (2.96 g, 11.29 mmol) (Method 195) and triphenylphosphine (3.11 g, 11.85 mmol) in THF (25 mL) was stirred at rt for 7 h. Water (2 mL, 111 mmol) was then added and the mixture then stirred at 50° C. overnight. The mixture was then concentrated under reduced pressure, and the residue was purified via silica gel chromatography (100% DCM to 37:3:60/methanol:Et₃N:DCM) to yield the title compound (2.96 g, 11.29 mmol) as an oil. ¹H NMR (400 MHz, Dichloromethane-d2) δ ppm 7.38-7.19 (m, 5H), 5.02 (s, 2H), 3.89 (brs, 1H), 3.62 (dd, 2H), 3.29-3.15 (m, 2H), 3.12-3.01 (m, 1H); m/z 236.

The following starting materials were prepared by the procedure of Method 193 using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 194

250

Method 195

(±)-benzyl 3-azido-4-hydroxypyrrolidine-1-carboxylate

To a stirred mixture of benzyl 6-oxa-3-azabicyclo[3.1.0]hexane-3-carboxylate (5.2 g, 23.72 mmol) (Method 197) in DMF (30 mL) was added sodium azide (2.313 g, 35.58 mmol) in a mixture of acetone (20.00 mL) and water (10.00 mL). The resulting mixture was stirred at 80° C. for 24 h. The mixture was then diluted with water and ether, separated, and the organic layer was washed with brine, dried and conc. invacuo. The resulting material was purified via silica gel chromatography (eluted with 15% to 35% ethyl acetate/hexane), yielding the title compound as a yellow solid (4.10 g, 65.9%). ¹H NMR (400 MHz, dichloromethane-d2) δ □ppm 7.50-7.25 (m, 5H), 5.43-5.25 (m, 2H), 4.31 (brs, 1H), 4.00 (brs, 1H), 3.77 (dd, 1H), 3.69 (dd, 1H), 3.53 (d, 1H), 3.44 (dd, 1H), 2.17 (brs, 1H); m/z 263.

The following starting materials were prepared by the procedure of Method 195 using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 196

276

Method 197

benzyl 6-oxa-3-azabicyclo[3.1.0]hexane-3-carboxylate

To a stirred mixture of benzyl 2,5-dihydro-1H-pyrrole-1-carboxylate (5 g, 24.60 mmol) in DCM (80 mL) was added 3-chlorobenzoperoxoic acid (6.89 g, 30.75 mmol) at 0° C. The mixture was then stirred at rt overnight. The reaction mixture was then filtered through a bed of Celite and the filtrate was washed with sat'd aqueous Na₂CO₃ and brine. The organic was then separated and dried over anhydrous Na₂SO₄, filtered, and conc. in vacuo to yield the title compound that was used for next step without further purification.

The following starting materials were prepared by the procedure of Method 197 using the appropriate starting materials.

Method Compound ¹H NMR m/z SM 198

217

Method 199

2-(3-amino-4-methylpiperidin-1-yl)-3-chlorobenzaldehyde

To a stirred solution of methyl 1-(2-chloro-6-formylphenyl)-4-methylpiperidin-3-ylcarbamate (220 mg, 0.71 mmol) (Method 119) in MeOH (3 ml) was added 40% aqueous solution of KOH (10 ml) drop wise, and the resulting mixture was heated at reflux for 16 h. The mixture was allowed to cool to rt, diluted with water, and extracted with DCM. The combined organic extract was dried over anhydrous Na₂SO₄, filtered, and conc. in vacuo. The resulting residue was purified via silica gel chromatography (100% ethyl acetate to 10% methanol in ethyl acetate) to yield the title compound as a light yellow gum (0.057 g, 31.9%). ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 8.44 (s, 1H), 7.40 (t, 2H), 7.07 (t, 1H), 4.29 (d, 1H), 3.58 (ddd, 1H), 3.38 (dd, 1H), 3.11 (td, 1H), 2.81 (d, 1H), 2.20-1.91 (m, 1H), 1.31-1.14 (m, 1H), 1.08 (d, 3H), 0.63-0.37 (m, 1H).

Method 200

2-fluoro-3-(2,2,2-trifluoroethoxy)benzonitrile

A stirred solution of 2-fluoro-3-hydroxybenzonitrile (800 mg, 5.83 mmol) in DMF (10 mL) at 0° C. was treated portionwise with NaH (280 mg, 7.00 mmol) (60% oil dispersion). The reaction mixture was then stirred for 0.5 h and 2,2,2-trifluoroethyl trifluoromethanesulfonate (1490 mg, 6.42 mmol) was added. The reaction was allowed to warm to rt overnight with stirring. The reaction mixture was then treated with water (40 mL) and brine (5 mL) and extracted with ethyl acetate (˜50 mL). The organic extract was washed with water, dried over anhydrous Na₂SO₄, filtered and conc. in vacuo to afford a residue which was purified via silica gel chromatography (10% to 30% ethyl acetate/hexanes) to yield the title compound as a white solid (1040 mg, 81%). ¹H NMR (400 MHz, MeOD) δ ppm 7.56 (td, 1H), 7.47-7.38 (m, 1H), 7.38-7.24 (m, 1H), 4.73 (q, 2H); m/z 220.

Method 201 (R)-tert-butyl 1-(3-formylbiphenyl-2-yl)piperidin-3-ylcarbamate

A mixture of (R)-tert-butyl 1-(2-bromo-6-formylphenyl)piperidin-3-yl carbamate (Method 95) (300 mg, 0.78 mmol), phenylboronic acid (143 mg, 1.17 mmol), 1,1′-bis(di-tert-butyllphosphino)ferrocenedichloro palladium (II) complex (40.8 mg, 0.06 mmol), and Na₂CO₃ (124 mg, 1.17 mmol) was suspended in dioxane (5 mL) and water (1.50 mL) and stirred at 110° C. for overnight under an atmosphere of nitrogen. The reaction was cooled to rt and water (˜50 mL) and ethyl acetate (˜50 mL) was added to the mixture. The organic phase was separated, dried over anhydrous MgSO₄, filtered, and conc. in vacuo affording a residue purified with via silica gel chromatography (40 g) using a gradient of 5% to 30% ethyl acetate/hexanes to yield the title compound as a low melting solid (168 mg, 56.4%); m/z 381.

One may purify examples provided above by reverse-phase HPLC in a solvent contain varying concentrations of trifluoroacetic acid or hydrochloric acid. Thus the examples above may be isolated as, the freebase, hydrochloride salt, or trifluoroacetate salt.

PIM 1 and 2 enzyme assay descriptions:

PIM1 In-Vitro Mobility Shift Assay

One may determine the activity of purified human His-PIM1 [2-313] enzyme in-vitro using a mobility shift assay on a Caliper LC3000 reader (Caliper, Mass.), which measures fluorescence of a phosphorylated and unphosphorylated FL-Ahx-Bad (FITC-(AHX)RSRHSSYPAGT-COOH, Primm 200606-00289, Primm Biotech, MA) and calculates a ratiometric value to determine percent turnover. One may express PIM1 (University of Dundee, Scotland) in baculovirus system with a typical yield of >85% purity.

One determines phosphorylation of the FL-Ahx-Bad in the presence and absence of the compound of interest. One preincubates 5 ul of Enzyme/Substrate/adenosine triphosphate (ATP) mix consisting of 2.4 nM PIM1, 3.6 uM FL-Ahx-Bad, and 240 uM ATP in 1.2× buffer with 2 ul of compound for 20 minutes at 25° C. One initiates reactions with 5 ul of Metal mix consisting of 24 mM MgCl₂ in 1.2× buffer and incubated at 25° C. for 90 minutes and stops the reactions by addition of 5 ul of Stop mix consisting of 100 mM HEPES, 121 mM ethylenediamine tetraacetic acid, 0.8% Coatin Reagent 3 (Caliper, Mass.), and 0.01% Tween. One detects phosphorylated and unphosphorylated substrate by a Caliper LC3000 reader (Caliper, Mass.) in the presence of separation buffer consisting of 100 mM HEPES, 16 mM ethylenediamine tetraacetic acid, 0.1% Coatin Reagent 3 (Caliper, Mass.), 0.015% Brij-35, 5% DMSO, and 5.6 mM MgCl₂. One may use the following separation conditions for a Caliper LC3000: −1.0 PSI, −2000 V upstream voltage, −400 V downstream voltage, 0.2 second sample sip, 45 second post sip, 10% laser strength.

PIM2 In-Vitro Mobility Shift Assay

One may determine the activity of purified human His-PIM2 [23-299] enzyme in-vitro using a mobility shift assay on a Caliper LC3000 reader (Caliper, Mass.), which measures fluorescence of a phosphorylated and unphosphorylated FL-Ahx-Bad (FITC-(AHX)RSRHSSYPAGT-COOH, Primm 200606-00289, Primm Biotech, MA) and calculates a ratiometric value to determine percent turnover. One may express PIM2 (produced at AstraZeneca, R&D Boston) in E. coli cells with a typical yield of >90% purity.

One determines phosphorylation of the FL-Ahx-Bad in the presence and absence of the compound of interest. One preincubates 5 uL of Enzyme/Substrate/adenosine triphosphate (ATP) mix consisting of 2.4 nM PIM1, 3.6 uM FL-Ahx-Bad, and 12 uM ATP in 1.2× buffer with 2 ul of compound for 20 minutes at 25° C. One initiates reactions with 5 uL of Metal mix consisting of 24 mM MgCl₂ in 1.2× buffer and incubates at 25° C. for 90 minutes. One stops reactions by addition of 5 uL of Stop mix consisting of 100 mM HEPES, 121 mM ethylenediamine tetraacetic acid, 0.8% Coatin Reagent 3 (Caliper, Mass.), and 0.01% Tween. One detects phosphorylated and unphosphorylated substrate by a Caliper LC3000 reader (Caliper, Mass.) in the presence of separation buffer consisting of 100 mM HEPES, 16 mM ethylenediamine tetraacetic acid, 0.1% Coatin Reagent 3 (Caliper, Mass.), 0.015% Brij-35, 5% DMSO, and 5.6 mM MgCl₂. One may use the following separation conditions for a Caliper LC3000: −1.0 PSI, −2000 V upstream voltage, −400 V downstream voltage, 0.2 second sample sip, 45 second post sip, 10% laser strength.

Using the above described assays, or appropriate modifications thereof, preferred compounds disclosed herein generally have an IC₅₀ for PIM1 of less that 5 micromolar (uM), and even more preferred of less than 1 micromolar. The table 1 below provides the percent inhibition of PIM1 at 0.3 micromolar for Examples provided herein. Several examples were tested more than once. Variations in the experimental outcomes, negative values, or values over 100% inhibition are presumably due to experimental error inherent in the assay.

TABLE 1 Example number PIM1 % I at 0.3 uM PIM2 % I at 0.3 uM  1 >95 77.7  2 >95 >95  3 >95 58.0  4 >95 79.6  5A >95 79.9  5B >95 >95  6 79.4 9.5  7 5.4 <5  8 16.3 <5  9 6.9 <5  10 >95 50.1  11 89.4 17.9  12 80.2 <5  13 <5 <5  14 >95 69.9  15 12.9 <5  16 13.9 <5  17 29.8 <5  18 <5 <5  19 >95 38.3  20 86.5 19.1  21 82.9 15.8  22 81.9 8.5  23 87.6 19.7  24 >95 44.3  25 >95 68.4  26 >95 49.6  27 90.8 25.1  28 90.0 27.4  29 92.8  30 90.8 15.6  31 92.8 23.3  32 94.0 31.9  33 >95 >95  34 >95 39.3  35 <5 <5  36 >95 >95  37 89.7 69.4  38 >95 92.9  39 89.4 57.3  40 >95 89.8  41 74.0 25.1  42 58.8 23.3  43 55.4 14.7  44 83.8 46.7  45 40.1 14.3  46 13.4 15.7  47 50.0 26.3  48 84.4 41.3  49 >95 86.3  50 93.7 45.4  51 85.5 80.6  52 >95 93.5  53 92.3 82.1  54 88.1 73.1  55 11.0 <5  56 90.7 33.5  57 53.3 42.6  58 16.0 7.1  59 <5 6.6  60 35.4 11.3  61 81.8 28.1  62 62.1 17.5  63 79.2 21.5  64 86.5 25.7  65 45.3 18.9  66 50.3 20.9  67 <5  68 41.9 9.0  69 <5 16.3  70 <5 <5  71 <5 <5  72 <5 <5  73 <5 <5  74  75 >95 69.5  76 59.6 27.6  77 51.2 22.7  78 71.4 30.0  79 90.0 60.7  80 <5  81 <5  82 87.4 58.2  83 34.0  84A 92.9 54.3  84B  84C 47.8 29.6  84D 27.8 <5  84E 11.7 6.1  84F 87.4 52.4  84G 92.0 63.0  84H >95 67.0  84I >95 82.7  84J 93.3 40.4  84K >95 90.8  84L >95 55.2  84M >95 80.9  84N 92.8 81.4  84O <5 <5  85 >95 82.4  86 >95 >95  87 >95 84.2  88 >95 88.4  89 48.4 21.5  90 78.2 86.3  91 6.6 16.4  92 >95 81.5  93 >95 66.3  94 >95 >95  95 >95 >95  96 >95 >95  97 >95 86.4  98 >95 81.4  99 >95 >95 100 93.0 93.9 101 >95 >95 102 93.3 91.9 103 >95 88.7 104 >95 92.4 105 >95 92.4 106 >95 92.8 107 >95 >95 108 >95 >95 109 64.2 65.8 110 90.9 90.7 111 >95 >95 112 >95 >95 113 >95 >95 114 >95 >95 115 93.3 76.1 116 >95 >95 117 >95 >95 118 >95 >95 119 56.2 20.1 120 58.9 22.2 121 >95 87.4 122A 88.2 55.0 122B >95 92.3 122C >95 >95 122D >95 >95 122E >95 >95 122F >95 >95 122G >95 92.1 122H >95 >95 122I >95 >95 122J >95 >95 122K >95 >95 122L >95 >95 122M >95 >95 122N >95 >95 1220 >95 >95 122P >95 >95 122Q >95 >95 122R >95 89.6 122S >95 >95 122T >95 >95 122U >95 >95 122V >95 >95 122W >95 >95 122X >95 67.1 122Y >95 >95 122Z >95 >95 122AA >95 >95 122AB >95 >95 122AC >95 >95 122AD >95 >95 122AE >95 >95 122AF 83.9 87.9 122AG 68.9 86.7 122AH 19.0 14.0 122AI >95 >95 123 >95 >95 124 >95 >95 125 >95 >95 126 >95 >95 127 >95 >95 128 81.2 49.0 129A >95 >95 129B >95 >95 129C 85.0 52.1 130 66.2 45.5 131A 27.1 <5 131B <5 <5 132 93.0 88.1 133 >95 93.4 134 >95 85.2 135 42.5 10.8 136 82.8 44.6 137 55.1 18.6 138 87.9 63.2 139 58.1 15.1 140 73.3 52.0 141 34.9 <5 142 86.1 75.2 143 85.9 49.8 144 63.2 21.2 145 >95 80.1 146 88.9 48.5 147 74.3 36.4 148 91.5 60.8 149 >95 75.4 150 79.8 31.7 151 31.5 13.5 152 10.8 16.7 

1. A method of treating or preventing cancer comprising, a) providing a pharmaceutical composition comprising a compound of formula I,

or salts thereof, functioning to inhibit a PIM kinase, wherein R¹ is selected from a carbocyclyl, aryl, and heterocyclyl, wherein R¹ is optionally substituted with one or more, the same or different, R²; R² is selected from halogen, C₁₋₆alkyl, halogenated C₁₋₆alkyl, amino, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, and heterocyclyl, wherein R² is optionally substituted with one or more, the same or different, R³; R³ is selected from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, carbocyclyl, aryl, and heterocyclyl, wherein R³ is optionally substituted with one or more, the same or different, R⁴; R⁴ is selected from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, carbocyclyl, aryl, and heterocyclyl, wherein R⁴ is optionally substituted with one or more, the same or different, R⁵; and R⁵ is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl; and b) administering said pharmaceutical composition to a subject diagnosed with, exhibiting symptoms of, or at risk for cancer.
 2. A compound of formula IA,

or salts thereof, wherein, --- is individually at each occurrence selected from a single and double bond; n is selected from 0, 1, or 2; m is selected from 0, 1, or 2; A is selected from N and CR⁷; X is selected from O, S, CHR¹⁰ and NR¹¹; Y is selected from N, CH, and C; R⁵, R⁶, and R⁷ are each individually and independently from hydrogen, C₁₋₆alkyl, halogen, cyano, nitro, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, carbocyclyl, aryl, and heterocyclyl, wherein R⁵, R⁶, and R⁷ are each optionally substituted with one or more, the same or different, R¹²; R⁸ and R⁹ are each individually and independently selected from hydrogen, amino, hydroxyl, mercapto, C₁₋₆alkyl, C₁₋₆alkylamino, carbocyclyl, aryl, and heterocyclyl wherein R⁸ and R⁹ are each optionally substituted with one or more, the same or different, R¹⁵; R¹⁰ is selected from hydrogen, C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfamoyl, arylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹⁰ is optionally substituted with one or more, the same or different, R¹²; R¹¹ is selected from hydrogen, formyl, C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, C₁₋₆alkoxycarbonyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹¹ is optionally substituted with one or more, the same or different, R¹²; R¹² is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹² is optionally substituted with one or more, the same or different, R¹⁶; R¹⁵ is selected from C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, and C₁₋₆alkoxycarbonyl wherein R¹⁵ is optionally substituted with one or more, the same or different, R¹²; R¹⁶ is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, aminomethyl, aminoethyl, aminopropyl, aminobutyl, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, and N-methyl-N-ethylsulfamoyl; and provided that R⁵, R⁶, and R⁷ are not all hydrogen and provided the compound is not 5-((5-nitro-2-(1-piperidinyl)phenyl)methylene)-2,4-thiazolidinedione or 5-((2-(4-morpholinyl)-5-nitrophenyl)methylene)-2,4-thiazolidinedione.
 3. A compound of formula IB,

or salts thereof, wherein, n is selected from 0, 1, or 2; X is selected from O, CHR¹⁰ and NR¹¹; R⁵, R⁶, and R⁷ are each individually and independently from hydrogen, halogen, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, and N-methyl-N-ethylsulfamoyl; R⁸ and R⁹ are each individually and independently selected from hydrogen, amino, C₁₋₆alkyl, C₁₋₆alkylamino, wherein R⁸ and R⁹ are each optionally substituted with one or more, the same or different, R¹⁵; R¹⁰ is selected from hydrogen, C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfamoyl, arylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹⁰ is optionally substituted with one or more, the same or different, R¹²; R¹¹ is selected from hydrogen, C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, C₁₋₆alkoxycarbonyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹¹ is optionally substituted with one or more, the same or different, R¹²; R¹² is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹² is optionally substituted with one or more, the same or different, R¹⁶; R¹⁵ is selected from C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, and C₁₋₆alkoxycarbonyl wherein R¹⁵ is optionally substituted with one or more, the same or different, R¹²; R¹⁶ is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, and N-methyl-N-ethylsulfamoyl.; and provided that said compound is not, 5-[[2-(4-methyl-1-piperazinyl)phenyl]methylene]-2,4-thiazolidinedione or 5-[[2-[(2,6-dichloro-3-methylphenyl)amino]phenyl]methylene]-2,4-thiazolidinedione.
 4. A compound of formula IC,

or salts thereof, wherein, n is selected from 0, 1, 2, 3, 4, 5, 6, 7 or 8; X is selected from O, S, CHR¹⁰ and NR¹¹; Y is selected from O, S, and NR¹³; R⁵, R⁶, and R⁷ are each individually and independently from C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, C₁₋₆dialkylamino, carbocyclyl, aryl, and heterocyclyl, wherein R⁵, R⁶, and R⁷ are each optionally substituted with one or more, the same or different, R¹²; R¹⁰ is selected from hydrogen, C₁₋₆alkyl, halogen, nitro, cyano, hydroxy, amino, mercapto, formyl, carboxy, carbamoyl, C₁₋₆alkoxy, C₁₋₆alkylthio, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, C₁₋₆alkylsulfamoyl, arylsulfamoyl, carbocyclyl, aryl, and heterocyclyl, wherein R¹⁰ is optionally substituted with one or more, the same or different, R¹²; R¹¹, R¹³, and R¹⁴ are each individually and independently selected from hydrogen, C₁₋₆alkyl, C₁₋₆alkanoyl, C₁₋₆alkylsulfinyl, C₁₋₆alkylsulfonyl, arylsulfonyl, and C₁₋₆alkoxycarbonyl wherein R¹¹ is optionally substituted with one or more, the same or different, R¹²; or R¹¹ and R¹⁴, taken together with the atoms to which they are attached form a five, six, or seven membered heterocyclic ring optionally substituted with one or more, the same or different, R¹²; R¹² is selected from halogen, nitro, cyano, hydroxy, trifluoromethoxy, trifluoromethyl, amino, formyl, carboxy, carbamoyl, mercapto, sulfamoyl, methyl, ethyl, methoxy, ethoxy, acetyl, acetoxy, methylamino, ethylamino, dimethylamino, diethylamino, N-methyl-N-ethylamino, acetylamino, N-methylcarbamoyl, N-ethylcarbamoyl, N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N-methyl-N-ethylcarbamoyl, methylthio, ethylthio, methylsulfinyl, ethylsulfinyl, mesyl, ethylsulfonyl, methoxycarbonyl, ethoxycarbonyl, N-methylsulfamoyl, N-ethylsulfamoyl, N,N-dimethylsulfamoyl, N,N-diethylsulfamoyl, N-methyl-N-ethylsulfamoyl, carbocyclyl, aryl, and heterocyclyl.
 5. A compound of formula IC as defined in claim 4, or salts thereof, wherein Y is NR¹³.
 6. A compound of formula IA, IB, or IC, as defined in claims 2-4, or salts thereof, wherein R⁵ is a halogenated C₁₋₆alkyl.
 7. A compound of formula IA, IB, or IC, as defined in claims 2-4, or salts thereof, wherein R⁷ is a halogen.
 8. A compound of formula IX,

or salts thereof, wherein R⁵ is selected from hydrogen, C₁₋₆alkoxy, carbamoyl, and halogenated C₁₋₆alkyl; R⁶ is selected from hydrogen, halogen, C₁₋₆alkoxy, and 2-(1-piperidyl)ethoxy; R⁷ is selected from hydrogen, halogen, and C₁₋₆alkoxy; R¹⁷ is a heterocarbocylcyl, wherein R¹⁷ is optionally substituted with one or more, the same or different, R¹⁸; R¹⁸ is selected from halogen, formyl, amino, C₁₋₆alkyl, C₁₋₆alkylamino, (C₁₋₆alkyl)₂amino, carbocyclyl, aryl, heterocyclyl, wherein R¹⁸ is optionally substituted with one or more, the same or different, R¹⁹; R¹⁹ is selected from amino, C₁₋₆alkyl, hydroxy, carbocyclyl, and heterocyclyl wherein R¹⁹ is optionally substituted with one or more, the same or different, R²⁰; and R²⁰ is selected from amino, C₁₋₆alkyl, and halogen.
 9. The compound of claim 8, or salts thereof, wherein, R¹⁷ is selected from (3R)-3-aminopyrrolidin-1-yl, (3R)-3-dimethylaminopyrrolidin-1-yl, (3S)-3-(3-aminopropylamino)pyrrolidin-1-yl, (3S)-3-(5-aminopentanoylamino)pyrrolidin-1-yl, (3S)-3-amino-1-piperidyl, (3S)-3-aminopyrrolidin-1-yl, (3S)-3-dimethylaminopyrrolidin-1-yl, (3S,5R)-3,5-dimethylpiperazin-1-yl, 1,4-diazepan-1-yl, 2-(1-piperidyl)ethoxy, 2-diethylaminoethoxy, 2-dimethylaminoethyl-methyl-amino, 2-hydroxyethoxy, 2-morpholinoethoxy, 3-(2-aminoethylamino)pyrrolidin-1-yl, 3-(2-hydroxyethylamino)pyrrolidin-1-yl, 3-(2-methylaminoethylamino)pyrrolidin-1-yl, 3-(3-aminopropanoylamino)pyrrolidin-1-yl, 3-(3-aminopropylamino)pyrrolidin-1-yl, 3-(3-methyl-1,2,4-oxadiazol-5-yl)-1-piperidyl, 3-(aminomethyl)-1-piperidyl, 3-(aminomethyl)pyrrolidin-1-yl, 3-acetamidopyrrolidin-1-yl, 3-aminopyrrolidin-1-yl, 3-dimethylaminopropoxy, 3-dimethylaminopropyl-methyl-amino, 3-dimethylaminopyrrolidin-1-yl, 3-hydroxypyrrolidin-1-yl, 3-pyridyl, 4-(1-methyl-4-piperidyl)piperazin-1-yl, 4-(1-piperidyl)-1-piperidyl, 4-(2-aminoethyl)piperazin-1-yl, 4-(2-hydroxyethyl)-1,4-diazepan-1-yl, 4-(2-hydroxyethyl)-1-piperidyl, 4-(2-hydroxyethyl)piperazin-1-yl, 4-(2-methylaminoethyl)piperazin-1-yl, 4-(2-morpholinoethyl)piperazin-1-yl, 4-(3-aminopropanoyl)-1,4-diazepan-1-yl, 4-(3-aminopropanoyl)piperazin-1-yl, 4-(3-aminopropyl)piperazin-1-yl, 4-(3-hydroxypropyl)piperazin-1-yl, 4-(3-methyl-1,2,4-oxadiazol-5-yl)-1-piperidyl, 4-(4-aminobutanoyl)-1,4-diazepan-1-yl, 4-(4-aminobutanoyl)piperazin-1-yl, 4-(4-chloro-2-fluoro-phenyl)piperazin-1-yl, 4-(4-fluorophenyl)piperazin-1-yl, 4-(4-pyridylmethyl)piperazin-1-yl, 4-(5-aminopentanoyl)-1,4-diazepan-1-yl, 4-(5-aminopentanoyl)piperazin-1-yl, 4-(azetidine-3-carbonyl)piperazin-1-yl, 4-(benzo[1,3]dioxol-5-ylmethyl)piperazin-1-yl, 4-(cyclopropylmethyl)piperazin-1-yl, 4-(hydroxymethyl)-1-piperidyl, 4-(piperidine-3-carbonyl)-1,4-diazepan-1-yl, 4-(piperidine-3-carbonyl)piperazin-1-yl, 4-(piperidine-4-carbonyl)piperazin-1-yl, 4-[(2-chlorophenyl)methyl]piperazin-1-yl, 4-[3-(aminomethyl)benzoyl]piperazin-1-yl, 4-[4-(piperazin-1-ylmethyl)benzoyl]piperazin-1-yl, 4-acetylpiperazin-1-yl, 4-amino-1-piperidyl, 4-butyl-1,4-diazepan-1-yl, 4-cyclopentylpiperazin-1-yl, 4-dimethylamino-1-piperidyl, 4-hydroxy-1-piperidyl, 4-isobutylpiperazin-1-yl, 4-isopropylpiperazin-1-yl, 4-methylpiperazin-1-yl, 4-morpholino-1-piperidyl, 4-pyridyl, 4-pyrrolidin-1-yl-1-piperidyl, 4-tert-butoxycarbonylpiperazin-1-yl, 4-tert-butylpiperazin-1-yl, morpholino, piperazin-1-yl, and pyrrolidin-1-yl.
 10. A compound selected from: 5-({2-[(3S)-3-aminopiperidin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-[(3R)-3-aminopiperidin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-{[2-(4-aminopiperidin-1-yl)-3-chloro-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-({2-[3-(aminomethyl)piperidin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-{[3-chloro-2-(1,4-diazepan-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-{[3-chloro-2-(4-cyclopentylpiperazin-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(4-fluorophenyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(3-methyl-1,2,4-oxadiazol-5-yl)piperidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-{[3-chloro-2-(4-pyrrolidin-1-ylpiperidin-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(1-methylethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(2-methylpropyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(2-hydroxyethyl)piperidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(4-chloro-2-fluorophenyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[3-(3-methyl-1,2,4-oxadiazol-5-yl)piperidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(pyridin-4-ylmethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(2-chlorobenzyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(1-methylpiperidin-4-yl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(2-morpholin-4-ylethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(cyclopropylmethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-{[3-chloro-2-(4-morpholin-4-ylpiperidin-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(3-hydroxypropyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(dimethylamino)piperidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-{[3-chloro-2-{[3-(dimethylamino)propyl] (methyl)amino}-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(2-hydroxyethyl)-1,4-diazepan-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-{[2-(4-butyl-1,4-diazepan-1-yl)-3-chloro-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-({3-chloro-2-[4-(2-hydroxyethyl)piperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-{[3-chloro-2-morpholin-4-yl-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-{[2-(4-tert-butylpiperazin-1-yl)-3-chloro-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-{[2-(1,4′-bipiperidin-1′-yl)-3-chloro-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-{[3-chloro-2-(4-methylpiperazin-1-yl)-5-(trifluoromethyl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; 5-({3-bromo-2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-[(3R,5S)-3,5-dimethylpiperazin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-chloro-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({3-[3-(4-methylpiperazin-1-yl)propoxy]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 2-{3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenoxy}acetamide; (5Z)-5-{[3-(3-piperidin-1-ylpropoxy)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; (5Z)-5-({3-[(4-methylpiperazin-1-yl)methyl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; N-[2-(dimethylamino)ethyl]-2′-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]-N-methylbiphenyl-4-sulfonamide; 5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-methoxyphenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-(trifluoromethyl)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({5-chloro-2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-4-methylphenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-fluorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-5-methylphenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({5-bromo-2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]-3-fluorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; 5-({2-chloro-6-[(3S)-3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-(aminomethyl)pyrrolidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-methoxyphenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-bromophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopyrrolidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-bromophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-ethoxyphenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(2-methylpropoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(cyclohexylmethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(cyclohexyloxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R,4R)-3-amino-4-hydroxypiperidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-{[3-chloro-2-(1,4-diazepan-1-yl)phenyl]methylidene}-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(1-methylethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-(1-methylethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-ethoxyphenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-{[4-(aminomethyl)benzyl]amino}piperidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({3-chloro-2-[(3R)-3-{[2-(methylamino)ethyl]amino}piperidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S,4S)-3-amino-4-hydroxypyrrolidin-1-yl]-3-chlorophenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({3-chloro-2-[4-methyl-3-(methylamino)piperidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-{[2-(3-amino-4-methylpiperidin-1-yl)-3-chlorophenyl]methylidene}-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(2,2,2-trifluoroethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-(2,2,2-trifluoroethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(2-methoxyethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3S)-3-aminopyrrolidin-1-yl]-3-(2-methoxyethoxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(cyclopentyloxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]-3-(cyclobutyloxy)phenyl}methylidene)-1,3-thiazolidine-2,4-dione; 4-[(3R)-3-aminopiperidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]benzamide; 4-[(3S)-3-aminopiperidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]benzamide; 4-[(3R)-3-aminopiperidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]benzoic acid; 4-[(3S)-3-aminopyrrolidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]benzoic acid; (5Z)-5-({2-[(3R)-3-aminopiperidin-1-yl]biphenyl-3-yl}methylidene)-1,3-thiazolidine-2,4-dione; 5-{[2-(3-aminopropoxy)-5-methoxyphenyl]methylidene}-1,3-thiazolidine-2,4-dione; N-{4-[3-(dimethylamino)pyrrolidin-1-yl]-3-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}acetamide; (5Z)-5-[(3-chloro-2-{(3R)-3-[(2-hydroxyethyl)amino]piperidin-1-yl}phenyl)methylidene]-1,3-thiazolidine-2,4-dione; (5Z)-5-[(3-chloro-2-{(3R)-3-[(3-hydroxypropyl)amino]piperidin-1-yl}phenyl)methylidene]-1,3-thiazolidine-2,4-dione; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-1-methyl-1H-imidazole-2-carboxamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-methoxyacetamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-1-methyl-1H-pyrazole-3-carboxamide; N²-carbamoyl-N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]glycinamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-pyridin-3-ylacetamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-pyridin-4-ylacetamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-1-methyl-1H-pyrazole-4-carboxamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-(1-oxidothiomorpholin-4-yl)acetamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-4-sulfamoylbutanamide; N′-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-N,N-dimethylbutanediamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-N˜2˜,N˜2˜-dimethylglycinamide; N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]-2-cyanoacetamide; N²-acetyl-N-[(3S)-1-{2-chloro-6-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl}pyrrolidin-3-yl]glycinamide; (5Z)-5-({3-chloro-2-[(3R)-3-(dipropylamino)piperidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; (5Z)-5-[(3-chloro-2-{(3R)-3-[(3,3,3-trifluoropropyl)amino]piperidin-1-yl}phenyl)methylidene]-1,3-thiazolidine-2,4-dione; 5-[(5-methoxy-2-{3-[(1-methylethyl)amino]propoxy}phenyl)methylidene]-1,3-thiazolidine-2,4-dione; (5Z)-5-({5-amino-2-[3-(dimethylamino)pyrrolidin-1-yl]phenyl}methylidene)-1,3-thiazolidine-2,4-dione; and 5-[(2-amino-4,5-dimethoxyphenyl)methylidene]-1,3-thiazolidine-2,4-dione, or salts thereof.
 11. A pharmaceutical composition comprising a compound of formula I, IA, IB, IC, or IX, as defined in claims 1-10, or a pharmaceutically acceptable salt thereof.
 12. A method of inhibiting a PIM kinase comprising, providing a compound of formula I, IA, IB, IC, or IX, as defined in claims 1-10 comprising mixing a PIM kinase and said compound under conditions such that PIM kinase phosphorylation is inhibited.
 13. The use of a compound of the formula I, IA, IB, IC, or IX, or a pharmaceutically acceptable salt thereof, as claimed in any one of claims 1-10, for the manufacture of a medicament for the production of an anti-cancer effect in a subject.
 14. A method of making a compound of formula IA as defined in claim 2,

or salt thereof, comprising a) mixing a compound of formula XI,

or salt thereof, wherein E is a halogen, and R⁵, R⁶, and A are defined in claim 2, with a compound of formula XII,

or salt thereof, wherein ---, R⁸, R⁹, n, m, Y and X are defined in claim 2, if Y is N, then R²¹ is hydrogen, if Y is C, then R²¹ is selected from boronic acid and a boronic ester, and if Y is CH, then R²¹ is selected from a metal halide, under conditions such that composition comprising a compound of formula XIII,

or salt thereof, is formed; and b) mixing the compound of formula XIII and thiazolidine-2,4-dione under conditions such that a compound of formula IA is formed.
 15. A method of making a compound of formula IC, or salt thereof comprising a) mixing a compound of formula V,

or salt thereof, wherein R⁵, R⁶, and R⁷ are defined in claim 4, with a compound of formula VI,

or salt thereof, wherein R¹⁴, n, X and Y are defined in claim 4, under conditions such that a composition comprising a compound of formula VIII,

or salt thereof, is formed; and b) mixing the compound of formula VIII and thiazolidine-2,4-dione under conditions such that a compound of formula IC is formed. 